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公开(公告)号：WO2021146443A1
公开(公告)日：2021-07-22
申请号：PCT/US2021/013465
申请日：2021-01-14
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.
Inventor： BERMAN, Russell , BENITEZ-MARZAN, Jaime Juan , POPOVICH, Natasha , RULISON, Aaron , SAXENA, Ravi
IPC: G01N21/03 , G01N21/64 , G01N21/77 , G01N21/6428 , G01N21/6452 , G01N2201/0873
Abstract: Provided herein are cartridges, packaged devices, and systems for improved nucleic acid sequencing. The cartridges, devices, and systems include a highly multiplexed optical chip comprising a plurality of nanoscale reaction regions that is configured to perform and report nucleic acid sequencing reactions. The chips are, in some embodiments, packaged for use in analytical nucleic acid sequencing reactions. The chips may be attached to a printed circuit board, may be surrounded by a protective enclosure, may include a flow cell, and may include optical features to minimize or block photobleaching of the sequencing reagents and background fluorescent signals. Also provided are analytical systems for nucleic acid sequencing that comprise the disclosed cartridges and packaged devices. The systems comprise an analytical instrument with electronic, optical, mechanical, fluidic, and/or thermal connectors designed to interact with the corresponding connectors on an associated cartridge or packaged device in a highly precise but reversible manner.
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Patent Agency Ranking
公开(公告)号：WO2014194028A1
公开(公告)日：2014-12-04
申请号：PCT/US2014/039917
申请日：2014-05-29
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC
Inventor： RULISON, Aaron , MCDONALD, Mark , LUNDQUIST, Paul
CPC classification number: G01N21/6452 , C12Q1/6874 , G01J3/2803 , G01J3/36 , G01N21/6428 , G01N21/75 , G01N2021/6471 , G01N2021/6478 , G01N2201/0633 , G02B3/0056 , G02B3/0062 , G02B5/045 , G02B13/006 , G02B13/0065 , G02B13/0085 , G02B27/1013
Abstract: Apparatus, systems and methods for use in analyzing discrete reactions are provided. The analytical devices of the invention use an array of nanoscale regions (a chip) that has discrete patches of nanoscale regions. The chip mates with a collection device comprising an array of compact lens trains (CLTs) where each of the CLTs corresponds to a single patch of nanoscale regions. Each CLT collects the emitted light from a patch on the chip, collimates the light, performs color separation on the collimated emitted light, and focuses the separated light onto a portion of pixels on the detector below the CLT. Such systems are useful for monitoring many analytical reactions at one time including single molecule sequencing reactions.
Abstract translation: 提供了用于分析离散反应的装置，系统和方法。 本发明的分析装置使用具有分散的纳米尺度区域的纳米尺度区域（芯片）阵列。 该芯片与包括紧凑型透镜列阵列（CLT）的收集装置配合，其中每个CLT对应于单个纳米尺度区域。 每个CLT收集来自芯片上的贴片的发射光，准直光，在准直发射的光上进行颜色分离，并将分离的光聚焦到CLT下方的检测器上的像素部分上。 这样的系统可用于一次监测许多分析反应，包括单分子测序反应。
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公开(公告)号：WO2016201387A1
公开(公告)日：2016-12-15
申请号：PCT/US2016/037106
申请日：2016-06-11
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.
Inventor： WANG, Shang , FOQUET, Mathieu , LUNDQUIST, Paul , RULISON, Aaron , MCDONALD, Mark , HERRMANN, Ariel
CPC classification number: G02B6/124 , G01N21/03 , G01N21/6452 , G01N21/648 , G01N21/6486 , G01N21/774 , G01N2021/0346 , G02B6/34 , G02B6/4221 , G02B2006/12135
Abstract: Integrated target waveguide devices and optical analytical systems comprising such devices are provided. The target devices include an optical coupler that is optically coupled to an integrated waveguide and that is configured to receive optical input from an optical source through free space, particularly through a low numerical aperture interface. The devices and systems are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices provide for the efficient and reliable coupling of optical excitation energy from an optical source to the optical reactions. Optical signals emitted from the reactions can thus be measured with high sensitivity and discrimination. The devices and systems are well suited for miniaturization and high throughput.
Abstract translation: 提供了包括这种装置的集成目标波导装置和光学分析系统。 目标器件包括光学耦合到集成波导的光耦合器，其被配置为从光源通过自由空间，特别是通过低数值孔径接口接收光输入。 这些装置和系统可用于高密度高分复用的光学反应，包括生化反应，如核酸测序反应。 这些器件提供了从光源到光学反应的光学激发能量的有效和可靠的耦合。 因此，可以以高灵敏度和辨别度测量从反应发射的光信号。 这些设备和系统非常适合小型化和高吞吐量。
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公开(公告)号：WO2016149397A1
公开(公告)日：2016-09-22
申请号：PCT/US2016/022684
申请日：2016-03-16
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.
Inventor： LUNDQUIST, Paul , MCDONALD, Mark , RULISON, Aaron
CPC classification number: C12Q1/6874 , G01N21/6452 , G01N21/648 , G01N21/7703 , G01N2201/06113 , G01N2201/08 , G02B6/00 , G02B6/122 , G02B6/34 , G02B2006/12147
Abstract: Optical delivery devices and integrated analytical systems comprising the optical delivery devices are provided. The optical delivery devices include optical inputs, optical outputs, and integrated optical waveguides that are configured for coupling of optical energy to a target waveguide device through free space. The integrated analytical systems include the optical delivery devices in combination with the target waveguide device. The devices and systems are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices provide for the efficient coupling of optical excitation energy from an optical source to the optical reactions. Optical signals emitted from the reactions can thus be measured with high sensitivity and discrimination. The devices and systems are well suited for miniaturization and high throughput.
Abstract translation: 提供了包括光学传送装置的光学传送装置和综合分析系统。 光学传送装置包括被配置为通过自由空间将光能耦合到目标波导装置的光输入，光输出和集成光波导。 集成分析系统包括与目标波导装置组合的光学传送装置。 这些装置和系统可用于高密度高分复用的光学反应，包括生化反应，如核酸测序反应。 这些器件提供了从光源到光学反应的光学激发能量的有效耦合。 因此，可以以高灵敏度和辨别度测量从反应发射的光信号。 这些设备和系统非常适合小型化和高吞吐量。
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公开(公告)号：WO2021168155A1
公开(公告)日：2021-08-26
申请号：PCT/US2021/018631
申请日：2021-02-18
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.
Inventor： KURITSYN, Alexey , MCDONALD, Mark , RULISON, Aaron , GROT, Annette
Abstract: Provided herein are highly multiplexed optical analytical systems for improved nucleic acid sequencing. The systems include a plurality of highly multiplexed optical chips, at least one optical source, and a plurality of optical delivery devices for illuminating an array of nanoscale rection regions on each of the optical chips. In use, the reaction regions contain fluorescent nucleic acid sequencing reagents and are arranged to report nucleic acid sequence information to optical detectors associated with the multiplexed optical chips in real time. The systems enable a massive increase in the scale of nucleic acid sequencing reactions capable of being performed within a single instrument without a corresponding increase in size, complexity, or cost of the instrument.
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6.发明申请
公开(公告)号：WO2019090192A1
公开(公告)日：2019-05-09
申请号：PCT/US2018/059112
申请日：2018-11-03
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.
Inventor： KURITSYN, Alexey , MCDONALD, Mark , RULISON, Aaron , BERMAN, Russell
CPC classification number: G02B6/12016 , G01N21/17 , G01N21/63 , G01N21/64 , G01N21/648 , G01N21/77 , G01N21/7703 , G02B6/122 , G02B6/124 , G02B6/34 , G02B6/422 , G02B6/4224 , G02B27/0955 , G02B27/0972 , G02B27/0977 , G02B2006/12147 , G02B2006/1215
Abstract: Provided herein are systems, devices, and methods for improved optical waveguide transmission and alignment in an analytical system. Waveguides in optical analytical systems can exhibit variable and increasing back reflection of single-wavelength illumination over time, thus limiting their effectiveness and reliability. The systems are also subject to optical interference under conditions that have been used to overcome the back reflection. Novel systems and approaches using broadband illumination light with multiple longitudinal modes have been developed to improve optical transmission and analysis in these systems. Novel systems and approaches for the alignment of a target waveguide device and an optical source are also disclosed.
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