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    LABELED NUCLEOTIDE ANALOGS, REACTION MIXTURES, AND METHODS AND SYSTEMS FOR SEQUENCING
    2.
    发明申请
    LABELED NUCLEOTIDE ANALOGS, REACTION MIXTURES, AND METHODS AND SYSTEMS FOR SEQUENCING 审中-公开
    标记的核苷酸类似物，反应混合物以及用于测序的方法和系统

    公开(公告)号：WO2017087975A1

    公开(公告)日：2017-05-26

    申请号：PCT/US2016/063178

    申请日：2016-11-21

    Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.

    Inventor： SEBO, Lubomir ,  SHEN, Gene ,  YUE, Stephen ,  OSUNA, Honey ,  LAPIN, Yuri ,  BROGLEY, Louis ,  FEDOROV, Andrei

    IPC: A61F2/06 ,  A61F2/82 ,  A61L31/08 ,  C08L67/02 ,  C08L67/04 ,  C08L67/06

    CPC classification number: C12Q1/6869 ,  C07H19/10 ,  C07K19/00 ,  C12Q2563/107

    Abstract: Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with high sensitivity at desirable wavelengths. They contain structural components that modulate the interactions of the analogs with DNA polymerase, thus decreasing photodamage and improving the kinetic and other properties of the analogs in sequencing reactions. Also provided are nucleotide and dye-labeled compounds of the subject analogs, as well as intermediates useful in the preparation of the compounds and analogs. Compositions comprising the compounds, methods of synthesis of the intermediates, compounds, and analogs, and mutant DNA polymerases are also provided.

    Abstract translation: 提供了包含至少一种抗生物素蛋白，至少一种染料标记的化合物和至少一种核苷酸化合物的标记的核苷酸类似物。 这些类似物可用于各种基于荧光的分析方法，包括高密度分析大量高度多重光学反应，如单分子实时核酸测序反应。 类似物在理想的波长处以高灵敏度可检测。 它们含有调节类似物与DNA聚合酶相互作用的结构成分，从而减少光损伤并改善测序反应中类似物的动力学和其他性质。 还提供了主题类似物的核苷酸和染料标记的化合物，以及可用于制备化合物和类似物的中间体。 还提供了包含所述化合物的组合物，所述中间体，化合物和类似物的合成方法以及突变型DNA聚合酶。

    MULTIMERIC PROTECTED FLUORESCENT REAGENTS
    3.
    发明申请
    MULTIMERIC PROTECTED FLUORESCENT REAGENTS 审中-公开
    多元保护荧光试剂

    公开(公告)号：WO2016126941A1

    公开(公告)日：2016-08-11

    申请号：PCT/US2016/016564

    申请日：2016-02-04

    Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.

    Inventor： ZHENG, Frank ,  HANES, Jeremiah ,  SHEN, Gene ,  BROGLEY, Louis ,  YUE, Stephen ,  LAPIN, Yuri ,  LYLE, John ,  OSUNA, Honey ,  FEDOROV, Andrei ,  SEBO, Lubomir

    IPC: A61K38/00 ,  A61K38/02 ,  C07K14/435 ,  C12Q1/68 ,  G01N33/58

    CPC classification number: C09B23/105 ,  C09B23/06 ,  C09B69/00 ,  C09B69/105 ,  G01N33/533

    Abstract: Multimeric protected fluorescent reagents and their methods of synthesis are provided. The reagents are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The reagents contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements. The reagents also contain coupling elements connect monomeric compounds into multimeric forms, thereby increasing brightness.

    Abstract translation: 提供了多聚体保护的荧光试剂及其合成方法。 这些试剂可用于各种基于荧光的分析方法，包括以高密度大量多重光学反应的分析，如单分子实时核酸测序反应。 试剂含有荧光染料元素，其允许以期望波长的高灵敏度检测化合物，允许化合物被目标生物分子特异性识别的结合元件以及减少荧光染料元件之间不希望的接触的保护屏蔽元件 和结合的靶生物分子，因此由荧光染料元素降低结合的靶生物分子的光损伤。 试剂还含有偶联元件将单体化合物连接成多聚体形式，从而增加亮度。

    MODIFIED BASE DETECTION WITH NANOPORE SEQUENCING
    5.
    发明申请
    MODIFIED BASE DETECTION WITH NANOPORE SEQUENCING 审中-公开
    用纳米级序列改性基底检测

    公开(公告)号：WO2013185137A1

    公开(公告)日：2013-12-12

    申请号：PCT/US2013/045009

    申请日：2013-06-10

    Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.

    Inventor： TURNER, Stephen ,  KORLACH, Jonas

    IPC: C12Q1/68 ,  C12N15/09

    CPC classification number: C12Q1/6869 ,  C12Q1/68 ,  C12Q1/6874 ,  G01N27/447 ,  G01N33/48721 ,  C12Q1/6827 ,  C12Q2521/513 ,  C12Q2521/543 ,  C12Q2565/631

    Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. Nanopore sequencing is performed using a processive enzyme to control the rate of translation of a single strand of a nucleic acid through a nanopore. The presence and identity of a modified base can be determined by monitoring the kinetics of translation through the pore even though the events that lead to the kinetic changes are separated in time and space from the translation of the modified base or it's compliment through the nanopore. The invention also comprises the use of hemi-genomic DNA in nanopore sequencing.

    Abstract translation: 提供了方法，组合物和系统用于表征修饰的核酸。 使用过程酶进行纳米孔测序以控制核酸的单链通过纳米孔的翻译速率。 即使导致动力学变化的事件在时间和空间上与经修饰的碱基的翻译或通过纳米孔的补充分离，也可以通过监测通过孔的翻译的动力学来确定改性碱基的存在和特性。 本发明还包括在半纳米孔测序中使用半基因组DNA。

    MICROMIRROR ARRAYS HAVING SELF ALIGNED FEATURES
    7.
    发明申请
    MICROMIRROR ARRAYS HAVING SELF ALIGNED FEATURES 审中-公开
    具有自对准特征的MICROMIRROR ARRAYS

    公开(公告)号：WO2011112260A2

    公开(公告)日：2011-09-15

    申请号：PCT/US2011/000452

    申请日：2011-03-10

    Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. ,  MONADGEMI, Pezhman

    Inventor： MONADGEMI, Pezhman

    IPC: G02B26/00 ,  G01N21/00

    CPC classification number: B29D11/00596 ,  B82Y20/00 ,  G02B1/118 ,  G02B5/09 ,  G02B5/26 ,  G02B6/3512 ,  G02B6/3556 ,  G02B6/3584 ,  G02B2207/101

    Abstract: Methods, arrays, and systems for the optical analysis of multiple chemical, biological, or biochemical reactions are provided. The invention includes methods for producing arrays of micromirrors on transparent substrates, each micromirror comprising a nanostructure or optical confinement on its top. The arrays are produced by a process in which lateral dimensions of both the nanostructures and micromirrors are defined in a single step, allowing for control of the relative placement of the features on the substrate, minimizing the process-related defects, allowing for improved optical performance and consistency. In some aspects, the invention provides methods of selectively etching large features on a substrate while not concurrently etching small features. In some aspects, the invention provides methods of etching large features on a substrate using hard mask materials.

    Abstract translation: 提供了用于多种化学，生物或生化反应的光学分析的方法，阵列和系统。 本发明包括在透明衬底上产生微镜阵列的方法，每个微反射镜在其顶部包括纳米结构或光学限制。 阵列是通过一个方法制造的，其中纳米结构和微反射镜的横向尺寸在单个步骤中被限定，允许控制特征在基底上的相对放置，最小化与工艺相关的缺陷，允许改进的光学性能 和一致性。 在一些方面，本发明提供了选择性地蚀刻衬底上的大特征而不同时蚀刻小特征的方法。 在一些方面，本发明提供了使用硬掩模材料在基底上蚀刻大特征的方法。

    SINGLE-MOLECULE REAL-TIME ANALYSIS OF PROTEIN SYNTHESIS
    9.
    发明申请
    SINGLE-MOLECULE REAL-TIME ANALYSIS OF PROTEIN SYNTHESIS 审中-公开
    单分子实时分析蛋白质合成

    公开(公告)号：WO2010144151A2

    公开(公告)日：2010-12-16

    申请号：PCT/US2010/001692

    申请日：2010-06-11

    Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. ,  FLUSBERG, Benjamin ,  TURNER, Stephen ,  KORLACH, Jonas ,  SCHADT, Eric

    Inventor： FLUSBERG, Benjamin ,  TURNER, Stephen ,  KORLACH, Jonas ,  SCHADT, Eric

    IPC: C12Q1/68

    CPC classification number: G01N33/542 ,  G01N33/6818 ,  Y10T436/13 ,  Y10T436/143333

    Abstract: The present invention is generally directed to compositions, methods, and systems for performing single-molecule, real-time analysis of analytical reactions in which protein synthesis is occurring. The ability to analyze such reactions provides an opportunity to study those reactions as well as to potentially identify factors and/or approaches for impacting such reactions, e.g., to either enhance, inhibit, or otherwise affect such reactions including, but not limited to, affecting the reaction rate, processivity, fidelity, duration, and the like.

    Abstract translation: 本发明一般涉及用于进行蛋白质合成发生的分析反应的单分子实时分析的组合物，方法和系统。 分析这些反应的能力提供了研究这些反应的机会，并且潜在地鉴定影响这些反应的因素和/或方法，例如增强，抑制或以其他方式影响这些反应，包括但不限于影响 反应速度，持续性，持续时间等。

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