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    SYSTEM FOR FERMENTATION OF BIOMASS FOR THE PRODUCTION OF ETHANOL
    2.
    发明申请
    SYSTEM FOR FERMENTATION OF BIOMASS FOR THE PRODUCTION OF ETHANOL 审中-公开
    用于生产乙醇的生物质发酵系统

    公开(公告)号：WO2010102063A2

    公开(公告)日：2010-09-10

    申请号：PCT/US2010/026125

    申请日：2010-03-03

    Applicant: POET RESEARCH, INC. ,  NARENDRANATH, Neelakantam V. ,  CARLSON, David Charles

    Inventor： NARENDRANATH, Neelakantam V. ,  CARLSON, David Charles

    IPC: C12P17/10

    CPC classification number: C12P7/10 ,  C12M21/12 ,  C12M23/58 ,  C12M43/02 ,  C12M45/02 ,  C12M45/04 ,  Y02E50/16 ,  Y02E50/17

    Abstract: A system for fermentation of biomass is disclosed. The system comprises a method for producing a fermentation product in a fermentation system from biomass that has been pre-treated and separated into a first component and a second component. The method comprises the steps of supplying the first component to the fermentation system; providing an ethanologen to the fermentation system; maintaining the first component and ethanologen in the fermentation system; and recovering the fermentation product from the fermentation system. A fermentation system configured to produce a fermentation product from biomass that has been pre-treated and separated into a first component and a second component is disclosed. The system comprises a first vessel configured to receive the first component and an ethanologen and a second vessel configured to propagate the ethanologen for supply to the first vessel. A biorefinery for producing a fermentation product from biomass is also disclosed. The biorefinery comprises a preparation system to prepare the biomass into prepared biomass; a pre-treatment system to pre-treat the prepared biomass with a dilute acid for separation into a first component from which pentose can accessed for fermentation and a second component from which hexose can be made available for fermentation; a first treatment system to treat the first component into a treated first component by removing removed components from the first component; a first fermentation system to produce a first fermentation product from the pentose; a distillation system to recover ethanol from the first fermentation product; and a treatment system to process removed components. The biomass comprises lignocellulosic material; the lignocellulosic material comprises at least one of corn cobs, corn plant husks, corn plant leaves and corn plant stalks. The first component comprises pentose; the pentose comprises xylose. The ethanologen is capable of fermenting xylose into ethanol.

    Abstract translation: 公开了一种用于发酵生物质的系统。 该系统包括在已经经过预处理并分离成第一组分和第二组分的生物质的发酵系统中产生发酵产物的方法。 该方法包括将第一组分供应至发酵系统的步骤; 为发酵系统提供一个种子学家; 保持发酵系统中的第一成分和乙素; 并从发酵系统中回收发酵产物。 公开了一种发酵系统，其被配置为从已经预处理并分离成第一组分和第二组分的生物质产生发酵产物。 该系统包括构造成接收第一部件的第一容器和配置成传播乙醇分离器以供给第一容器的第二容器和第二容器。 还公开了用于从生物质产生发酵产物的生物精炼厂。 生物精炼包括将生物质制备成制备的生物质的制备系统; 预处理系统，用稀酸预处理所制备的生物质，以分离成可以从其中进入发酵的第一组分，以及可以从其中使己糖可用于发酵的第二组分; 第一处理系统，通过从第一组分去除去除的组分来将第一组分处理成经处理的第一组分; 从戊糖生产第一发酵产物的第一发酵系统; 从第一发酵产物回收乙醇的蒸馏系统; 以及处理去除的部件的处理系统。 生物质包括木质纤维素材料; 木质纤维素材料包括玉米芯，玉米植物壳，玉米植物叶和玉米植物茎中的至少一种。 第一组分包括戊糖; 戊糖包括木糖。 乙醇酵母能够将木糖发酵成乙醇。

    SYSTEM FOR MANAGEMENT OF YEAST TO FACILITATE THE PRODUCTION OF ETHANOL
    3.
    发明申请
    SYSTEM FOR MANAGEMENT OF YEAST TO FACILITATE THE PRODUCTION OF ETHANOL 审中-公开
    YEAST管理系统，以促进乙醇的生产

    公开(公告)号：WO2014193344A1

    公开(公告)日：2014-12-04

    申请号：PCT/US2013/042927

    申请日：2013-05-28

    Applicant: POET RESEARCH, INC. ,  NARENDRANATH, Neelakantam V. ,  CARLSON, David Charles

    Inventor： NARENDRANATH, Neelakantam V. ,  CARLSON, David Charles

    IPC: C12N1/18 ,  C12N1/16 ,  C12M1/00

    CPC classification number: C12N1/18 ,  C12M21/12 ,  C12M43/02 ,  C12P7/10 ,  Y02E50/16

    Abstract: A system and method for managing an ethanologen for use in biorefinery is disclosed. The method for propagating ethanologen for use in the production of a fermentation product from biomass comprises the steps of providing a medium for propagation of ethanologen; supplying a first cell mass of ethanologen to the medium; supplying xylose to the medium as a carbon source for the ethanologen; and maintaining the medium comprising the first cell mass of ethanologen at a pH of between about 5.0 and 6.0 and at a temperature of between about 26 and About 37 degrees Celsius so that the first cell mass of ethanologen is propagated into a second cell mass of ethanologen. The second cell mass of ethanologen is larger than the first cell mass of ethanologen. The system for propagating ethanologen for use in the production of a fermentation product from biomass comprises a first stage comprising a first vessel configured to maintain a medium comprising ethanologen; a second stage comprising a second vessel configured to maintain a medium supplied from the first stage; a source of xylose to be provided to the medium as a carbon source for ethanologen in the first stage; and a source of xylose to be provided to the medium as a carbon source for the ethanologen in the second stage. The ethanologen has a first cell mass when supplied to the first stage and the ethanologen has a second cell mass when supplied from the first stage to the second stage and the ethanologen has a third cell mass when supplied from the second stage. The second cell mass is at least 200 times larger than the first cell mass The third cell mass is at least 20 times larger than the second cell mass.

    Abstract translation: 公开了一种用于生物炼制中用于管理种子学的系统和方法。 用于从生物质生产发酵产物中用于繁殖的酿酒酵母的方法包括以下步骤：提供用于繁殖的培养基; 向培养基中提供第一个细胞质量的木质素; 向培养基提供木糖作为乙醇的碳源; 并且将包含乙醇提取物的第一细胞团的培养基保持在约5.0至6.0之间的温度和约26至约37摄氏度之间的温度下，使得第一细胞团的乙醇培养基传播到第二细胞团的乙醇培养基 。 乙醇生物的第二个细胞团大于第一个细胞团的乙型动物。 用于从生物质生产发酵产物的用于繁殖的酿酒酵母的系统包括第一阶段，其包括构造成维持包含酿酒酵母的培养基的第一容器; 第二级，包括构造成维持从第一级供应的介质的第二容器; 在第一阶段提供作为乙醇生物碳源的培养基中的木糖来源; 以及作为第二阶段的乙醇生物的碳源提供给培养基的木糖来源。 当从第一阶段供应时，乙醇提取物具有第一细胞团，并且当从第一阶段供应时，乙醇生物具有第二细胞团，并且当从第二阶段供应时，所述乙醇培养物具有第三细胞团。 第二细胞质量比第一细胞质量大至少200倍。第三细胞质量比第二细胞团质量至少大20倍。

    SYSTEMS AND METHODS FOR IMPROVING ETHANOL YIELD
    4.
    发明申请
    SYSTEMS AND METHODS FOR IMPROVING ETHANOL YIELD 审中-公开
    改善乙醇含量的系统和方法

    公开(公告)号：WO2012125739A1

    公开(公告)日：2012-09-20

    申请号：PCT/US2012/029093

    申请日：2012-03-14

    Applicant: POET RESEARCH, INC. ,  LEWIS, Stephen M. ,  NARENDRANATH, Neelakantam V.

    Inventor： LEWIS, Stephen M. ,  NARENDRANATH, Neelakantam V.

    IPC: C12P7/06

    CPC classification number: C12P7/08 ,  C12P7/06 ,  Y02E50/17

    Abstract: Systems and methods for improving ethanol yield are provided. A feedstock is ground suitably fine for use in fermentation. The feedstock may include corn or any other suitable material. In some cases, the feedstock undergoes a fractionation prior to grinding. The ground feedstock may be slurried with water and enzymes to facilitate conversion of the starch in the feedstock to sugars. The slurry may be about 35% solids. After being slurried, an ethanologen may be added. Additionally, the pH of the slurry may be adjusted to between 4.2 and 5.2 to facilitate the priming. A primer is added to the slurry. The primer may include any weak acid, and in some embodiments includes acetic acid. Acetic acid, when used as a primer, may be added at a concentration of between 1200 and 3600 parts per million. The slurry is fermented to produce improved yields of ethanol.

    Abstract translation: 提供了提高乙醇产量的系统和方法。 将原料粉碎成适用于发酵的原料。 原料可以包括玉米或任何其它合适的材料。 在一些情况下，原料在研磨之前进行分馏。 地面原料可以用水和酶进行浆化，以促进原料中的淀粉转化为糖。 浆料可以是约35％的固体。 在酝酿之后，可能会添加一个种族主义者。 此外，浆料的pH可以调节到4.2和5.2之间以便于起动。 向浆料中加入底漆。 引物可以包括任何弱酸，并且在一些实施方案中包括乙酸。 当用作底漆时，乙酸可以以1200至3600ppm的浓度加入。 将浆料发酵以产生改善的乙醇产率。

    SYSTEMS AND METHODS FOR YEAST PROPAGATION
    8.
    发明申请
    SYSTEMS AND METHODS FOR YEAST PROPAGATION 审中-公开
    用于YEAST传播的系统和方法

    公开(公告)号：WO2014160184A1

    公开(公告)日：2014-10-02

    申请号：PCT/US2014/025990

    申请日：2014-03-13

    Applicant: POET RESEARCH, INC.

    Inventor： NARENDRANATH, Neelakantam V. ,  LEWIS, Stephen M.

    IPC: C12N1/16

    CPC classification number: C12M45/09 ,  C12M21/18 ,  C12N1/16 ,  C12N1/18

    Abstract: The present invention relates to systems and methods for propagating yeast using a feedstock material such as starch and one or more enzymes (e.g., an enzyme cocktail) to break down the feedstock material into one or more monosaccharides at a sufficient rate so that the yeast can use the monosaccharides as a carbon source for producing more yeast cells while not producing an undue amount of alcohol.

    Abstract translation: 本发明涉及使用原料如淀粉和一种或多种酶（例如，酶混合物）传播酵母的系统和方法，以将酵母以足够的速率将原料分解成一种或多种单糖，以使酵母可以 使用单糖作为碳源生产更多的酵母细胞，同时不产生不适当量的酒精。

    SYSTEMS AND METHODS FOR FEEDSTOCK QUALITY ASSESSMENT
    10.
    发明申请
    SYSTEMS AND METHODS FOR FEEDSTOCK QUALITY ASSESSMENT 审中-公开
    用于饲料质量评估的系统和方法

    公开(公告)号：WO2012078885A2

    公开(公告)日：2012-06-14

    申请号：PCT/US2011/063963

    申请日：2011-12-08

    Applicant: POET RESEARCH, INC. ,  LEWIS, Stephen M. ,  NARENDRANATH, Neelakantam V.

    Inventor： LEWIS, Stephen M. ,  NARENDRANATH, Neelakantam V.

    IPC: C12Q1/44

    CPC classification number: C12Q1/44 ,  G01N33/10 ,  G01N2333/415

    Abstract: Assessing quality of feedstock is provided and may be useful for determining quality of feed (such as corn kernels). The assessment determines endogenous enzyme activity within the feedstock, which correlates with total ethanol yields in raw starch hydrolysis (non-cooked) systems. In some embodiments, a sample containing flour (ground feedstock) is provided. In some cases, flour is diluted in water and buffered with a phosphate buffered solution. The buffered flour solution is contacted with a molecule, or fluorescent dye, such as fluorescein diacetate and/or difluorofluorescein that is altered by enzymatic (esterase) activity in a detectable fashion. Enzyme activity may be facilitated using an incubator. The detectible alteration may be measured using a fluorometer. In some embodiments, two incubations and fluorescence measurements can be performed. By subtracting the difference between the measured fluorescence, a fluorescence index may be found, which correlates with the endogenous enzyme activity in the feedstock sample.

    Abstract translation: 提供原料质量评估，可用于确定饲料质量（如玉米粒）。 该评估确定原料内的内源酶活性，其与生淀粉水解（未煮熟）体系中的总乙醇产量相关。 在一些实施方案中，提供含有面粉（研磨原料）的样品。 在某些情况下，将面粉稀释在水中并用磷酸盐缓冲溶液缓冲。 缓冲的面粉溶液与分子或荧光染料接触，例如以可检测的方式通过酶（酯酶）活性改变的荧光素二乙酸酯和/或二氟荧光素。 使用培养箱可以促进酶活性。 可以用荧光计测量可检测的变化。 在一些实施方案中，可以进行两次孵育和荧光测量。 通过减去测量的荧光之间的差异，可以发现与原料样品中的内源性酶活性相关的荧光指数。

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