VALORISATION DE L'ACETONE PAR PROCEDE DE FERMENTATION IBE IMPLIQUANT DES MICROORGANISMES GENETIQUEMENT MODIFIES

    公开(公告)号:WO2022002625A1

    公开(公告)日:2022-01-06

    申请号:PCT/EP2021/066514

    申请日:2021-06-17

    摘要: La présente invention concerne un procédé de production d'alcools comprenant: a. une étape de fermentation en présence d'un microorganisme génétiquement modifié, mettant en œuvre au moins une section réactionnelle comprenant un bioréacteur alimenté par un flux d'alimentation, une zone de séparation, une zone d'analyse du moût, de sorte que: - phase 1: lorsque la teneur en acétone dudit moût est inférieure à 1 g/L, ledit flux d'alimentation comprend une solution aqueuse de sucres et un flux acétone, de sorte que la concentration en acétone dudit flux d'alimentation est inférieure ou égale à 10 g/L; - phase 2: lorsque la teneur en acétone dudit moût est supérieure ou égale à 1 g/L, ledit flux d'alimentation consiste en ladite solution aqueuse de sucres; b. une étape de traitement du moût pour séparer un effluent acétone et un effluent aqueux d'alcools; c. une étape de recyclage d'au moins une fraction dudit effluent acétone vers l'étape a) pendant la phase 1.

    IMPROVED MICROORGANISMS FOR ARABINOSE FERMENTATION

    公开(公告)号:WO2021231621A1

    公开(公告)日:2021-11-18

    申请号:PCT/US2021/032054

    申请日:2021-05-12

    发明人: TASSONE, Monica

    IPC分类号: C12N9/00 C12P7/04 C12P7/14

    摘要: Described herein are recombinant host organisms having an active arabinose fermentation pathway and further comprising a heterologous polynucleotide a heterologous polynucleotide encoding a L-xylulose reductase (LXR). Also described are processes for 5 producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant host organisms.

    A YEAST STAGE TANK INCORPORATED FERMENTATION SYSTEM AND METHOD

    公开(公告)号:WO2021072344A1

    公开(公告)日:2021-04-15

    申请号:PCT/US2020/055174

    申请日:2020-10-11

    申请人: LEE TECH LLC

    发明人: LEE, Chie Ying

    IPC分类号: C12P7/06 C12P7/14

    摘要: Methods of and system for growing and maintaining an optimized/ideal active yeast solution in the yeast tank and fermenter tank during the fermentation filling cycle are provided. A new yeast stage tank is used between the yeast tank and the fermenter tank allowing yeast to rapidly produce a huge amount of active young yeast cells for a fermenter during the filling period. A measurable and useful controlling factor, %DT/%Yeast by weight ratio (or "food" to yeast ratio), is used (e.g., %DT = glucose), which offers information on the health status of the yeast. The controlling factor is used to control the status of the yeast throughout the entire process.

    SYSTEMS AND METHODS FOR CONTINUOUSLY FERMENTING C5 AND C6 SACCHARIDES
    6.
    发明申请
    SYSTEMS AND METHODS FOR CONTINUOUSLY FERMENTING C5 AND C6 SACCHARIDES 审中-公开
    用于连续引发C5和C6糖链的系统和方法

    公开(公告)号:WO2017205705A1

    公开(公告)日:2017-11-30

    申请号:PCT/US2017/034596

    申请日:2017-05-26

    摘要: This invention provides optimized fermentation of cellulosic and hemicellulosic sugars. Biomass-derived hemicellulosic and cellulosic sugars are independently conditioned and separately fermented, utilizing reuse and recycle of microorganisms, metabolic intermediates, and nutrients. Conditioned sugars can be fermented in separate vessels, where excess cells from glucose fermentation are conveyed to hemicellulose sugar fermentation along with raffinate from solvent recovery, to enhance productivity and product yield. Some variations provide a method of fermenting C 5 and C 6 sugars to fermentation products, the method comprising: fermenting a C 6 -rich sugar feed to a first fermentation product; fermenting a C 5 -rich sugar feed to a second fermentation product; removing microorganism cells from the first fermentor, to maintain a cell concentration within a selected range; conveying microorganism cells to a second fermentor; and removing microorganism cells from the second fermentor, to maintain a microorganism cell concentration that is greater than that in the C 6 -rich fermentor.

    摘要翻译: 本发明提供了纤维素和半纤维素糖的优化发酵。 利用微生物,代谢中间体和营养物质的再利用和再循环,将生物质衍生的半纤维素和纤维素糖独立调节并分别发酵。 条件糖可以在单独的容器中发酵,其中来自葡萄糖发酵的过量细胞与来自溶剂回收的提余液一起被输送到半纤维素糖发酵,以提高生产率和产品产量。 一些变化提供了将C 5和C 6糖发酵成发酵产物的方法,所述方法包括:发酵富含C 6的糖进料 到第一发酵产物; 将第C 5高丰度糖原料发酵成第二发酵产物; 从第一发酵罐中除去微生物细胞,以将细胞浓度保持在选定范围内; 将微生物细胞输送至第二发酵罐; 并从第二发酵罐中除去微生物细胞,以保持比富含Cys6的发酵罐更高的微生物细胞浓度。

    PROCESSES FOR CONSUMING ACETIC ACID DURING FERMENTATION OF CELLULOSIC SUGARS, AND PRODUCTS PRODUCED THEREFROM
    7.
    发明申请
    PROCESSES FOR CONSUMING ACETIC ACID DURING FERMENTATION OF CELLULOSIC SUGARS, AND PRODUCTS PRODUCED THEREFROM 审中-公开
    糖尿病发酵过程中消耗醋酸的方法及其生产的产品

    公开(公告)号:WO2016160707A1

    公开(公告)日:2016-10-06

    申请号:PCT/US2016/024523

    申请日:2016-03-28

    摘要: This invention provides a way to deal with acetic acid derived from biomass, for fermentation of cellulosic sugars. In some variations, a process for producing ethanol from lignocellulosic biomass comprises: extracting hemicelluloses and acetic acid from lignocellulosic biomass; hydrolyzing the hemicelluloses, using an acid catalyst or enzymes, to generate hemicellulose monomers and more acetic acid; fermenting acetic acid to lipids using a suitable lipid-producing microorganism, thereby reducing acetic acid concentration; fermenting hemicellulose monomers to ethanol using a suitable ethanol-producing microorganism; and recovering the ethanol. The co-fermentation of acetic acid and sugars may be carried out in a single fermentor or in separate fermentors. The invention may be applied to fermentation products other than ethanol. In some embodiments, the fermentation product can act as an extraction solvent to extract lipids from the lipid-producing microorganism, such as a lipid-producing yeast.

    摘要翻译: 本发明提供一种处理来自生物质的乙酸,用于发酵纤维素糖的方法。 在一些变型中,从木质纤维素生物质生产乙醇的方法包括:从木质纤维素生物质中提取半纤维素和乙酸; 使用酸催化剂或酶水解半纤维素以产生半纤维素单体和更多的乙酸; 使用合适的产生脂质的微生物将乙酸发酵至脂质,从而降低乙酸浓度; 使用合适的乙醇生产微生物将半纤维素单体发酵成乙醇; 并回收乙醇。 乙酸和糖的共发酵可以在单个发酵罐中或在单独的发酵罐中进行。 本发明可以应用于乙醇以外的发酵产物。 在一些实施方案中,发酵产物可以起提取溶剂的作用,从产生脂质的微生物如脂质生产酵母提取脂质。

    PROCESSES FOR BIOCONVERTING SYNGAS TO OXYGENATED HYDROCARBONACEOUS COMPOUNDS
    8.
    发明申请
    PROCESSES FOR BIOCONVERTING SYNGAS TO OXYGENATED HYDROCARBONACEOUS COMPOUNDS 审中-公开
    生物合成氧化烯烃化合物的方法

    公开(公告)号:WO2016160526A1

    公开(公告)日:2016-10-06

    申请号:PCT/US2016/024061

    申请日:2016-03-24

    申请人: SYNATA BIO, INC.

    发明人: HICKEY, Robert

    摘要: Continuous processes for the anaerobic bioconversion of syngas to oxygenated hydrocarbonaceous products, in particular lower alkanols, are disclosed in which nutrients, including micronutrients, and lower carboxylate anion are recovered from at least a portion of an aqueous distillation fraction from a distillation unit operation to recover lower alkanols by using a "tight" ultrafiltration membrane. At least about 75 percent of the water permeates the ultrafiltration membrane. The tight ultrafiltration membrane rejects sufficient components that are adverse to the microorganisms used in the bioconversion that continuous fermentation operations over long durations can be achieved.

    摘要翻译: 公开了将合成气厌氧生物转化为含氧烃类产物,特别是低级链烷醇的连续方法,其中从蒸馏单元操作的至少一部分含水蒸馏馏分回收营养物质,包括微量营养素和低级羧酸根阴离子,以回收 通过使用“紧”超滤膜来降低链烷醇。 至少约75%的水渗入超滤膜。 紧密的超滤膜排斥了对生物转化中使用的微生物不利的足够的组分,可以实现长时间持续的发酵操作。