摘要:
La présente invention concerne un procédé de production d'alcools comprenant: a. une étape de fermentation en présence d'un microorganisme génétiquement modifié, mettant en œuvre au moins une section réactionnelle comprenant un bioréacteur alimenté par un flux d'alimentation, une zone de séparation, une zone d'analyse du moût, de sorte que: - phase 1: lorsque la teneur en acétone dudit moût est inférieure à 1 g/L, ledit flux d'alimentation comprend une solution aqueuse de sucres et un flux acétone, de sorte que la concentration en acétone dudit flux d'alimentation est inférieure ou égale à 10 g/L; - phase 2: lorsque la teneur en acétone dudit moût est supérieure ou égale à 1 g/L, ledit flux d'alimentation consiste en ladite solution aqueuse de sucres; b. une étape de traitement du moût pour séparer un effluent acétone et un effluent aqueux d'alcools; c. une étape de recyclage d'au moins une fraction dudit effluent acétone vers l'étape a) pendant la phase 1.
摘要:
Described herein are recombinant host organisms having an active arabinose fermentation pathway and further comprising a heterologous polynucleotide a heterologous polynucleotide encoding a L-xylulose reductase (LXR). Also described are processes for 5 producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant host organisms.
摘要:
Methods of and system for growing and maintaining an optimized/ideal active yeast solution in the yeast tank and fermenter tank during the fermentation filling cycle are provided. A new yeast stage tank is used between the yeast tank and the fermenter tank allowing yeast to rapidly produce a huge amount of active young yeast cells for a fermenter during the filling period. A measurable and useful controlling factor, %DT/%Yeast by weight ratio (or "food" to yeast ratio), is used (e.g., %DT = glucose), which offers information on the health status of the yeast. The controlling factor is used to control the status of the yeast throughout the entire process.
摘要:
The present disclosure relates to methods for improving the nutritional quality of fermentation by-products derived from starch-containing material, wherein the fermentation step is carried out in the presence of a first enzyme composition comprising at least one hemicellulase, and wherein the process comprises further the steps of i) subjecting the fermented mash after the fermentation to a second enzyme composition comprising a beta- 1,3 glucanase and a xylanase, ii) separating the desired fermentation product by distillation.
摘要:
The invention relates to a polysaccharide-oxidizing composition comprising a polysaccharide-oxidizing enzyme wherein, when the said polysaccharide-oxidizing enzyme is compared to the reference polypeptide of SEQ ID NO. 1 as described in the specification by using the BLAST-P comparison method, (i) the said polysaccharide- oxidizing enzyme possesses an amino acid identity of 20 % or more with the said reference polypeptide and (ii) the BLAST-P comparison method results in an E-value of 10 e -3 or less.
摘要翻译:
本发明涉及一种多糖 - 氧化组合物包含多糖氧化酶,其特征在于,当所述多糖氧化酶与SEQ ID NO:2的参照多肽。 1如通过使用BLAST-P的比较方法中,(i)在本说明书中所描述的,所述氧化多糖酶具有20%以上的氨基酸同一性与所述参照多肽和(ii)的BLAST-P的比较方法的结果 E值为10 e -3或更低。 p>
摘要:
This invention provides optimized fermentation of cellulosic and hemicellulosic sugars. Biomass-derived hemicellulosic and cellulosic sugars are independently conditioned and separately fermented, utilizing reuse and recycle of microorganisms, metabolic intermediates, and nutrients. Conditioned sugars can be fermented in separate vessels, where excess cells from glucose fermentation are conveyed to hemicellulose sugar fermentation along with raffinate from solvent recovery, to enhance productivity and product yield. Some variations provide a method of fermenting C 5 and C 6 sugars to fermentation products, the method comprising: fermenting a C 6 -rich sugar feed to a first fermentation product; fermenting a C 5 -rich sugar feed to a second fermentation product; removing microorganism cells from the first fermentor, to maintain a cell concentration within a selected range; conveying microorganism cells to a second fermentor; and removing microorganism cells from the second fermentor, to maintain a microorganism cell concentration that is greater than that in the C 6 -rich fermentor.
摘要:
This invention provides a way to deal with acetic acid derived from biomass, for fermentation of cellulosic sugars. In some variations, a process for producing ethanol from lignocellulosic biomass comprises: extracting hemicelluloses and acetic acid from lignocellulosic biomass; hydrolyzing the hemicelluloses, using an acid catalyst or enzymes, to generate hemicellulose monomers and more acetic acid; fermenting acetic acid to lipids using a suitable lipid-producing microorganism, thereby reducing acetic acid concentration; fermenting hemicellulose monomers to ethanol using a suitable ethanol-producing microorganism; and recovering the ethanol. The co-fermentation of acetic acid and sugars may be carried out in a single fermentor or in separate fermentors. The invention may be applied to fermentation products other than ethanol. In some embodiments, the fermentation product can act as an extraction solvent to extract lipids from the lipid-producing microorganism, such as a lipid-producing yeast.
摘要:
Continuous processes for the anaerobic bioconversion of syngas to oxygenated hydrocarbonaceous products, in particular lower alkanols, are disclosed in which nutrients, including micronutrients, and lower carboxylate anion are recovered from at least a portion of an aqueous distillation fraction from a distillation unit operation to recover lower alkanols by using a "tight" ultrafiltration membrane. At least about 75 percent of the water permeates the ultrafiltration membrane. The tight ultrafiltration membrane rejects sufficient components that are adverse to the microorganisms used in the bioconversion that continuous fermentation operations over long durations can be achieved.
摘要:
The present technology provide a method of dewatering whole stillage. The addition of a xylanase in combination with a pectinase results in a wet cake with a higher dry mass. The advantage here is less energy consumption while drying.
摘要:
Lactic acid bacterial cultures, cell populations and articles of manufacture comprising same are disclosed for generating ethanol from lignocellulse.