公开(公告)号：WO2022226305A1

公开(公告)日：2022-10-27

申请号：PCT/US2022/025947

申请日：2022-04-22

Applicant: PRECISION BIOSCIENCES, INC. ,  UNIVERSITY OF MIAMI

Inventor： SMITH, James Jefferson ,  TOMBERLIN, Ginger ,  MORRIS, John ,  SHOOP, Wendy ,  MORAES, Carlos T.

IPC: C12N9/22 ,  A61K8/14 ,  A61K8/66

Abstract: Disclosed herein are recombinant meganucleases engineered to recognize and cleave a recognition sequence present in the human mitochondrial DNA (mtDNA). The disclosure further relates to the use of such recombinant meganucleases in methods for producing genetically-modified eukaryotic cells, and to a population of genetically-modified eukaryotic cells wherein the mtDNA has been having modified or edited.