公开(公告)号：WO2022226303A1

公开(公告)日：2022-10-27

申请号：PCT/US2022/025945

申请日：2022-04-22

Applicant: PRECISION BIOSCIENCES, INC. ,  UNIVERSITY OF MIAMI

Inventor： MORAES, Carlos T. ,  SMITH, James Jefferson ,  JANTZ, Derek ,  TOMBERLIN, Ginger H. ,  MORRIS, John ,  SHOOP, Wendy

IPC: C12N9/22 ,  A61K38/43

Abstract: Disclosed herein are recombinant meganucleases engineered to recognize and cleave a recognition sequence present in the human mitochondrial DNA (mtDNA). The disclosure further relates to the use of such recombinant meganucleases in combination with mitochondrial transit peptides in methods for producing genetically-modified eukaryotic cells, and to a population of genetically-modified eukaryotic cells wherein the mtDNA has been modified or edited.

公开(公告)号：WO2022226305A1

公开(公告)日：2022-10-27

申请号：PCT/US2022/025947

申请日：2022-04-22

Applicant: PRECISION BIOSCIENCES, INC. ,  UNIVERSITY OF MIAMI

Inventor： SMITH, James Jefferson ,  TOMBERLIN, Ginger ,  MORRIS, John ,  SHOOP, Wendy ,  MORAES, Carlos T.

IPC: C12N9/22 ,  A61K8/14 ,  A61K8/66

Abstract: Disclosed herein are recombinant meganucleases engineered to recognize and cleave a recognition sequence present in the human mitochondrial DNA (mtDNA). The disclosure further relates to the use of such recombinant meganucleases in methods for producing genetically-modified eukaryotic cells, and to a population of genetically-modified eukaryotic cells wherein the mtDNA has been having modified or edited.

公开(公告)号：WO2022226316A1

公开(公告)日：2022-10-27

申请号：PCT/US2022/025965

申请日：2022-04-22

Applicant: PRECISION BIOSCIENCES, INC. ,  NORTH CAROLINA STATE UNIVERSITY

Inventor： DEWEY, Ralph ,  JANTZ, Derek ,  SMITH, James Jefferson ,  TOMBERLIN, Ginger H. ,  MORRIS, John ,  MATTHIADIS, Anna ,  SHOOP, Wendy

IPC: C12N9/22 ,  C12N15/82

Abstract: Disclosed herein are recombinant meganucleases engineered to bind and cleave a recognition sequence in the mitochondrial DNA (mtDNA) of a eukaryotic cell, such as a plant cell. The disclosure further relates to the use of such recombinant meganucleases in methods for producing genetically-modified eukaryotic cells, and to a population of genetically-modified eukaryotic cells wherein the mtDNA has been having modified or edited.