公开(公告)号：WO2023010219A1

公开(公告)日：2023-02-09

申请号：PCT/CA2022/051194

申请日：2022-08-05

申请人： RAPID NOVOR, INC.

发明人： LE BIHAN, Thierry ,  NUNEZ DE VILLAVICENCIO DIAZ, Teresa ,  LIYASOVA, Mariya ,  YAO, Chenyu ,  LIU, Qixin ,  REITZEL, Chelsea ,  DUAN, Jin ,  TAYLOR, Paul ,  MA, Bin

IPC分类号： C12Q1/37 ,  C07K1/14 ,  C07K1/18 ,  C07K16/00 ,  G01N30/02 ,  G01N30/60 ,  G01N30/72 ,  G01N33/53 ,  G01N33/543

摘要： The present application relates to methods for determining antibody heavy and light chain pairing in an antibody and/or antibody fragment mixture. The methods include a step of submitting the mixture to a separation step under non-reducing condition to obtain isolated antibodies or antibody fragments, or of contacting the mixture with a protein cross-linking agent. The isolated or cross-linked antibodies and/or antibody fragments are then digested using one or more proteases to generate antibody or antibody fragment peptides, which are then analyzed for example by mass spectrometry (MS) to determining heavy chain and light chain pairing of the antibody or antibody fragment in the mixture.

公开(公告)号：WO2021248244A1

公开(公告)日：2021-12-16

申请号：PCT/CA2021/050791

申请日：2021-06-10

申请人： RAPID NOVOR, INC.

发明人： LE BIHAN, Thierry ,  MA, Bin ,  TAYLOR, Paul ,  YAO, Chenyu ,  LIU, Qixin ,  REITZEL, Chelsea ,  GOROSPE, Kayle Kathleen Marie ,  LIYASOVA, Mariya

IPC分类号： C07K16/42 ,  C07K1/107 ,  C07K1/22 ,  C07K16/00 ,  C07K7/06 ,  C07K7/08 ,  C12P21/06 ,  C07K16/06 ,  C07K16/065 ,  C07K2317/34 ,  C07K2317/565

摘要： Methods and reagents to obtaining a sample enriched in peptides comprising the third complementarity-determining region of the heavy chain (CDRH3) of immunoglobulins, such as IgGs, are described. These methods are based on the use of targeted protease digestion of immunoglobulins and affinity purification of CDRH3 peptides using specific antibodies. Such methods and reagents are useful for analyzing the immunoglobulin repertoire.