公开(公告)号：WO2015054554A1

公开(公告)日：2015-04-16

申请号：PCT/US2014/059993

申请日：2014-10-10

Applicant: REGENERON PHARMACEUTICALS, INC.

Inventor： LAWRENCE, Shawn ,  KIM, Ann ,  JOHNSON, Amy

IPC: C12N5/02 ,  C12N5/16

CPC classification number: C12N5/0682 ,  C07K16/00 ,  C07K2317/14 ,  C12N5/00 ,  C12N2500/60 ,  C12N2511/00 ,  C12P21/02

Abstract: An improved method for large scale production of proteins and/or polypeptides in cell culture is provided. In accordance with the present invention, the method provides for culturing cells that have metabolically shifted. The use of such a method or system allows high levels of protein or polypeptide production and reduces accumulation of unwanted metabolic waste such as lactate. Proteins and polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, immunogenic, or other commercial biologic compositions, such as antibodies.

Abstract translation: 提供了用于在细胞培养物中大规模生产蛋白质和/或多肽的改进方法。 根据本发明，该方法提供培养具有代谢转移的细胞。 使用这种方法或系统允许高水平的蛋白质或多肽产生并减少不期望的代谢废物如乳酸盐的积累。 根据本发明表达的蛋白质和多肽可有利地用于制备药物，免疫原性或其他商业生物组合物，例如抗体。

公开(公告)号：WO2021112924A1

公开(公告)日：2021-06-10

申请号：PCT/US2020/046823

申请日：2020-08-18

Applicant: REGENERON PHARMACEUTICALS, INC.

Inventor： LAWRENCE, Shawn ,  JOHNSON, Amy ,  CASEY, Meghan ,  MASTROGIACOMO, Jaimie ,  WANG, Shunhai ,  LI, Ning

IPC: C12N5/00

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：WO2019010191A1

公开(公告)日：2019-01-10

申请号：PCT/US2018/040734

申请日：2018-07-03

Applicant: REGENERON PHARMACEUTICALS, INC.

Inventor： CHEN, John ,  LAWRENCE, Shawn ,  JOHNSON, Amy ,  LONEY, Theodore ,  PANGULE, Ravindra ,  HANG, Ta-Chun ,  CARVER, Scott ,  SCHILLING, Bernhard

IPC: C07K14/415 ,  C12N5/00 ,  C12N15/09 ,  C12P21/00 ,  C12P21/06

Abstract: The instant application provides a method for screening batches of soy hydrolysate for a desired amount of a component thereof, such as ornithine or putrescine, and selecting only those batches of soy hydrolysate that have a desired amount of such component. The present disclosure also sets forth methods for culturing cells in media supplemented with selected batches of soy to produce more consistent, high quality lots of a protein of interest. Further, the present disclosure provides a plurality of protein preparations that have each been produced by culturing cells in media supplemented with separate batches of soy hydrolysate containing a desired amount of ornithine or putrescine, whereby each batch of protein produced exhibits improved quality of the protein of interest or amount of quality protein produced.

公开(公告)号：WO2021112929A1

公开(公告)日：2021-06-10

申请号：PCT/US2020/046862

申请日：2020-08-18

Applicant: REGENERON PHARMACEUTICALS, INC.

Inventor： TUSTIAN, Andrew ,  LAWRENCE, Shawn ,  JOHNSON, Amy ,  CASEY, Meghan ,  MASTROGIACOMO, Jaimie ,  VARTAK, Ankit ,  DALY, Thomas ,  PYLES, Erica ,  PALACKAL, Nisha ,  WANG, Shunhai ,  LI, Ning ,  CHEN, Hunter ,  BHUPENDER BHALLA, Amardeep Singh ,  FRANKLIN, Matthew

IPC: C07K14/71

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：WO2014144198A1

公开(公告)日：2014-09-18

申请号：PCT/US2014/028501

申请日：2014-03-14

Applicant: REGENERON PHARMACEUTICALS, INC.

Inventor： OSHODI, Shadia ,  JOHNSON, Amy ,  LAWRENCE, Shawn

IPC: C12N5/00

CPC classification number: C12P21/00 ,  C07K16/18 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/005 ,  C12N2500/32 ,  C12N2500/46 ,  C12N2510/02 ,  C12N2511/00

Abstract: The specification describes an improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Ornithine, or a combination of ornithine and putrescine can be added to serum-free media or chemically defined media to improve viable cell density, to reduce cell doubling time, and to increase the production of a protein of interest.

Abstract translation: 本说明书描述了一种改良的无血清动物细胞培养基，可用于生产目标蛋白。 鸟氨酸或鸟氨酸和腐胺的组合可添加到无血清培养基或化学成分确定的培养基中，以改善活细胞密度，减少细胞倍增时间，并增加感兴趣的蛋白质的产生。

公开(公告)号：WO2021113591A1

公开(公告)日：2021-06-10

申请号：PCT/US2020/063238

申请日：2020-12-04

Applicant: REGENERON PHARMACEUTICALS, INC.

Inventor： MARTIN, Joel ,  DAVIS, Samuel ,  LAWRENCE, Shawn ,  JOHNSON, Amy ,  CASEY, Meghan ,  MASTROGIACOMO, Jaimie ,  WANG, Shunhai ,  LI, Ning ,  TUSTIAN, Andrew ,  VARTAK, Ankit ,  FRANKLIN, Matthew

IPC: A61K35/761

Abstract: The present invention provides VEGF mini-trap molecules and method of treating or preventing angiogenic disorders such as angiogenic eye disorders and cancer.