GENETICALLY MODIFIED PLANTS HAVING MODULATED BRASSINOSTEROID SIGNALING
    1.
    发明申请
    GENETICALLY MODIFIED PLANTS HAVING MODULATED BRASSINOSTEROID SIGNALING 审中-公开
    具有调节型BROSINOSTEROID信号的遗传修饰植物

    公开(公告)号:WO0055302A3

    公开(公告)日:2001-01-11

    申请号:PCT/US0006915

    申请日:2000-03-16

    Abstract: The present invention provides cytochrome P450s useful for producing genetically modified plants characterized as having the phenotypic trait of modulated brassinolide synthesis of signaling, for example, resulting in insect resistance, dwarfism and darker-green foliage compared with wild type plants. Such plants can be modified, for example, using "bas1", or functional homologues thereof, a polypeptide encoded by bas1 that modulates brassinolide synthesis and/or signaling in plants. The invention also provides methods for modulating ecdysteroid activity in a plant and for assaying brassinosteroid function in a plant. The latter method can be used to create a gain-of-function allelic series of plants characterized by increasing levels of overexpression of a cytochrome P450 to screen for brassinolide activity in plant species.

    Abstract translation: 本发明提供可用于生产经遗传修饰的植物的细胞色素P450,其特征在于具有调节的油菜素内酯合成信号的表型性状,例如与野生型植物相比,导致昆虫抗性,矮化和深绿色叶。 这样的植物可以被修饰,例如使用“bas1”或其功能同系物,由bas1编码的多肽,其调节油菜素内酯合成和/或在植物中的信号转导。 本发明还提供了调节植物中蜕皮类固醇活性和测定植物中油菜素类固醇功能的方法。 后一种方法可用于产生功能增强的等位基因系列,其特征在于增加细胞色素P450的过表达水平以筛选植物物种中的油菜素内酯活性。

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