公开(公告)号：WO2018052593A1

公开(公告)日：2018-03-22

申请号：PCT/US2017/046554

申请日：2017-08-11

申请人： CALIFORNIA COOPERATIVE RICE RESEARCH FOUNDATION, INC.

发明人： MCKENZIE, Kent Scheidel

IPC分类号： C12N15/00

CPC分类号： A01H5/10 ,  A01H1/02 ,  A01H4/00 ,  A01H6/4636 ,  C07K14/325 ,  C07K14/415 ,  C12N9/001 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8278 ,  C12N15/8279 ,  C12N15/8286

摘要： The present invention is directed toward rice lines containing mutant allele ROXY that exhibit non-transgenic resistance to the herbicide oxyfluorfen. The invention relates to the seeds, plants and plant parts of rice plants containing mutant allele ROXY and to methods for producing a rice plant by crossing rice plants containing mutant allele ROXY with itself or another rice cultivar. The invention further relates to methods for producing a rice plant containing mutant allele ROXY containing in its genetic material one or more transgenes and to the transgenic rice plants and plant parts produced by those methods. This invention also relates to methods for producing other rice cultivars, lines or plant parts derived from rice plants containing mutant allele ROXY and to the rice plants, varieties, and their parts derived from the use of those methods. The invention further relates to transferring mutant allele ROXY to different genetic backgrounds.

摘要翻译： 本发明涉及含有显示出对除草剂乙氧氟草醚的非转基因抗性的突变等位基因ROXY的水稻品系。 本发明涉及含有突变等位基因ROXY的稻植物的种子，植物和植物部分，以及通过将含有突变等位基因ROXY的稻植物与其自身或另一种稻品种杂交来产生稻植物的方法。 本发明进一步涉及用于产生含有在其遗传物质中含有一种或多种转基因的突变等位基因ROXY的水稻植物的方法，以及由这些方法产生的转基因稻植物和植物部分的方法。 本发明还涉及用于产生其他水稻品种，来源于含有突变等位基因ROXY的水稻植物的系或植物部分的方法，以及来自使用这些方法的水稻植物，品种及其部分的方法。 本发明进一步涉及将突变等位基因ROXY转移到不同的遗传背景。

公开(公告)号：WO2017103582A1

公开(公告)日：2017-06-22

申请号：PCT/GB2016/053929

申请日：2016-12-14

申请人： ROTHAMSTED RESEARCH LIMITED

发明人： KANYUKA, Konstantin ,  CYRILLE, Saintenac ,  CAMBON, Florence ,  LANGIN, Thierry ,  LEE, Wing-Sham ,  HAMMOND-KOSACK, Kim E.

IPC分类号： C12N15/82

CPC分类号： C12N15/8279

摘要： The present invention relates generally to the fields of genetic engineering and plant molecular biology. Specifically, the invention concerns nucleic acids and polypeptides which confer fungal disease resistance in plants, in particular, resistance to Septoria tritici blotch (STB) disease. Accordingly, the invention relates to isolated nucleic acids and polypeptides which are capable of conferring resistance to the STB disease and the use thereof in generating and identifying STB resistant plants. The invention also encompasses expression vectors comprising said polynucleotides and plants exhibiting resistance to STB disease. The nucleic acids, polypeptides, expression vectors, plants and methods of the present invention may usefully find application in agricultural biotechnology.

摘要翻译： 本发明一般涉及基因工程和植物分子生物学领域。 具体而言，本发明涉及在植物中赋予真菌疾病抗性的核酸和多肽，特别是对小麦小斑枯病（Septoria tritici blotch）（STB）疾病的抗性。 因此，本发明涉及能够赋予STB疾病抗性的分离的核酸和多肽及其在产生和鉴定STB抗性植物中的用途。 本发明还包括包含所述多核苷酸的表达载体和显示出对STB疾病的抗性的植物。 本发明的核酸，多肽，表达载体，植物和方法可以有用地用于农业生物技术。

公开(公告)号：WO2017037255A1

公开(公告)日：2017-03-09

申请号：PCT/EP2016/070759

申请日：2016-09-02

申请人： GENOPLANTE-VALOR

发明人： HIREL, Bertrand ,  PEREZ, Pascual

IPC分类号： C12N15/82 ,  C12N15/52 ,  A01H5/00

CPC分类号： C12N15/8279 ,  C12N9/93 ,  C12N15/8261 ,  C12N15/8271 ,  C12N15/8274 ,  C12Y603/01002 ,  Y02A40/146

摘要： The invention relates to a method for improving the yield of a plant, comprising overexpressing in said plant a glutamine synthetase, said glutamine synthetase being both constitutively overexpressed in said plant and specifically overexpressed in the bundle sheath cells of said plant. The invention also relates to means for obtaining plants with improved yield.

摘要翻译： 本发明涉及一种用于提高植物产量的方法，其包括在所述植物中过表达谷氨酰胺合成酶，所述谷氨酰胺合成酶在所述植物中组成型过表达并且在所述植物的束鞘细胞中特异性过表达。 本发明还涉及获得具有提高的产量的植物的方法。

公开(公告)号：WO2016170547A1

公开(公告)日：2016-10-27

申请号：PCT/IN2016/050116

申请日：2016-04-21

申请人： METAHELIX LIFE SCIENCES LTD.

发明人： AGARWAL, Sandhya ,  SHIVAPPA, Sarala ,  SAMPATH, Mukundan ,  MOHANDOSS, Sheba Jennifer ,  PRASANNAKUMAR, Kammaragattae Vaishanava ,  SARANGI, Rinku Ranjan ,  VAIRAMANI, Ramanathan ,  KOTTARAM KRISHNADAS, Narayanan

IPC分类号： C12N15/82 ,  C12N15/11

CPC分类号： C12N15/8279 ,  C12N9/1051 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/8218 ,  C12N15/8282 ,  C12Y204/01016 ,  C12Y301/03016 ,  C12Y302/01028

摘要： The present invention discloses novel DNA construct composition to provide strain non-specific and/or race non-specific broad spectrum tolerance against phytopathogen in host plant. The DNA construct composition comprises ofat least one novel RNAi construct, at least one promoter and at least one transcriptional terminator so designed to produce RNAi gene construct to down-regulate pathogen specific gene target by way of RNAi mediated gene silencing and consequently inhibiting growth of phytopathogen. The RNAi gene constructs are designed for both single gene targets and multi-gene targets. This invention also discloses method of preparing novel DNA construct and the method to control phytopathogens.

摘要翻译： 本发明公开了提供宿主植物中对植物病原体的非特异性和/或竞争性非特异性广谱耐受性的新型DNA构建组合物。 DNA构建组合物包含至少一种新型RNAi构建体，至少一种启动子和至少一种转录终止子，其设计用于产生RNAi基因构建体，以通过RNAi介导的基因沉默方式下调病原体特异性基因靶标，从而抑制植物病原体的生长 。 RNAi基因构建体设计用于单基因靶标和多基因靶标。 本发明还公开了制备新型DNA构建体的方法和控制植物病原体的方法。

公开(公告)号：WO2016066748A1

公开(公告)日：2016-05-06

申请号：PCT/EP2015/075127

申请日：2015-10-29

申请人： NUNHEMS B.V.

发明人： SCHAAREMAN, Robert Theodorus Gerardus ,  THOMAS, Vincent Pierre Andre ,  VAN DER AREND, Adrianus J. M. ,  RAEDTS, Rob ,  RODRİGUEZ, Olga Julián

IPC分类号： A01H5/12 ,  C07K14/415 ,  C12N15/82

CPC分类号： A01H5/12 ,  C07K14/415 ,  C12N15/8279

摘要： The present invention relates to the field of lettuce breeding, in particular to Quantitative Trait Loci for resistance against the lettuce aphid Nasonovia ribisnigri biotype Nr: 1 and to cultivated lettuce comprising one or more of these Quantitative Trait Loci.

摘要翻译： 本发明涉及莴苣育种领域，特别涉及抗莴苣ap ri生物型Nr：1的定量性状位点和包含这些定量性状基因座中的一种或多种的培养的莴苣。

公开(公告)号：WO2016043588A1

公开(公告)日：2016-03-24

申请号：PCT/NL2015/050646

申请日：2015-09-17

申请人： WAGENINGEN UNIVERSITEIT

发明人： VOSSEN, Jacobus Hubertus ,  JO, Kwang Ryong ,  VISSER, Richard Gerardus Franciscus

IPC分类号： A01H5/06 ,  C07K14/415 ,  C12N15/82

CPC分类号： C12N15/8279 ,  A01H1/02 ,  A01H5/06 ,  C07K14/415 ,  C07K16/16 ,  C12N15/8282

摘要： The invention relates to a new gene that produces a protein which is capable of inferring oomycete resistance, more preferably resistance to Phytophthora infestans when expressed in a plant, wherein said nucleotide sequence encodes a protein that is encoded by the protein produced by the nucleotide sequence of Fig.10 or the protein depicted in Fig. 11 or a nucleotide sequence that codes for a protein that has an identity of at least 95% with said protein produced by the nucleotide sequence of Fig. 10 or the protein depicted in Fig. 11. The invention also relates to a method for providing at least partial resistance or increasing resistance in a plant against an oomycete infection comprising providing a plant or a part thereof with a nucleotide sequence as indicated above or a functional fragment thereof, preferably wherein said plant is a plant from the Solanaceae family, more preferably Solanum tuberosum .

摘要翻译： 本发明涉及产生蛋白质的新基因，该蛋白质能够推测出卵菌的抗性，更优选在植物中表达时对致病疫霉的抗性，其中所述的核苷酸序列编码由由核苷酸序列产生的蛋白质编码的蛋白质 图10或图10所示的蛋白质。 11或编码与图1的核苷酸序列产生的蛋白质具有至少95％同一性的蛋白质的核苷酸序列。 10或图1所示的蛋白质。 本发明还涉及一种用于在植物中对抗卵菌感染提供至少部分抗性或增加抗性的方法，包括提供植物或其部分与上述核苷酸序列或其功能片段，优选其中所述植物 是来自茄科的植物，更优选是马铃薯（Solanum tuberosum）。

公开(公告)号：WO2015195921A9

公开(公告)日：2016-03-10

申请号：PCT/US2015036439

申请日：2015-06-18

申请人： UNIV CALIFORNIA

发明人： RONALD PAMELA ,  SCHWESSINGER BENJAMIN ,  PRUITT RORY ,  ANNA JOE ,  WEIGUO ZHANG

IPC分类号： C12Q1/68

CPC分类号： C12N15/8279 ,  A01N63/02 ,  C07K14/195 ,  C07K14/415 ,  C12N15/8281 ,  C12P21/02

摘要： Provided herein are compounds and methods for increasing disease resistance and/or root length in plants.

公开(公告)号：WO2016007606A2

公开(公告)日：2016-01-14

申请号：PCT/US2015/039520

申请日：2015-07-08

申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH

发明人： HIND, Sarah, R. ,  MARTIN, Gregory, B. ,  STRICKLER, Susan, R.

IPC分类号： A01H5/00 ,  C12N15/82

CPC分类号： C12N15/8279 ,  C07K14/415 ,  C12N15/8218 ,  C12N15/8281 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/158

摘要： One aspect of the present invention relates to a nucleic acid construct that includes a nucleic acid molecule that encodes FLAGELLIN-SENSING 3 ("FLS3") protein; a 5' heterologous DNA promoter sequence; and a 3 ' terminator sequence, where the nucleic acid molecule, the DNA promoter sequence, and the terminator sequence are operatively coupled to permit transcription of the nucleic acid molecule. The present invention also relates to a method of imparting disease resistance to a plant. This method involves transforming a plant or a plant seed with a nucleic acid molecule that increases expression of an FLS3 protein, where said transforming is effective in imparting disease resistance to the transformed plant or to a transgenic plant produced from the transformed plant seed. The present invention also relates to methods of expressing a nucleic acid molecule in a plant, identifying a candidate plant suitable for breeding that displays enhanced disease resistance, and enhancing efficiency of transformation of a plant by Agrobacterium .

摘要翻译： 本发明的一个方面涉及核酸构建体，其包含编码FLAGELLIN-SENSING 3（“FLS3”）蛋白质的核酸分子; 5'异源DNA启动子序列; 和3'终止子序列，其中核酸分子，DNA启动子序列和终止子序列可操作地偶联以允许核酸分子的转录。 本发明还涉及赋予植物抗病性的方法。 该方法包括用增加FLS3蛋白表达的核酸分子转化植物或植物种子，其中所述转化有效赋予转化植物或由转化植物种子产生的转基因植物的抗病性。 本发明还涉及在植物中表达核酸分子的方法，鉴定适于显示增强的抗病性的育种的候选植物，以及通过土壤杆菌提高植物转化的效率。 p>

公开(公告)号：WO2015189376A1

公开(公告)日：2015-12-17

申请号：PCT/EP2015/063144

申请日：2015-06-12

申请人： SESVANDERHAVE N.V.

发明人： WEYENS, Guy ,  LEFÈBVRE, Marc ,  THAMMANNAGOWDA, Shivegowda ,  LOMMEL, Murielle

IPC分类号： C07K14/415 ,  C12N15/82

CPC分类号： C12N15/821 ,  A01H1/02 ,  A01H1/04 ,  C12N9/88 ,  C12N15/52 ,  C12N15/8209 ,  C12N15/8241 ,  C12N15/8279 ,  C12N15/8281 ,  C12N15/8282 ,  C12N15/8285 ,  C12N15/8286

摘要： The present invention is related to a method for the transformation of sugar beet protoplasts comprising the steps of: – obtaining protoplasts from stomatal guard cells isolated from a sugar beet plant, - transforming the protoplasts with a nucleic acid construct comprising a nucleotide sequence of interest and a selection marker sequence, - applying to an in vitro culture of said protoplasts, one or more ALS inhibitors at a concentration that is lethal to the in vitro culture of the protoplasts and - regenerating sugar beet plants from the surviving protoplasts having integrated the nucleic acid construct comprising the sequence of interest and the selection marker sequence, wherein the selectionmarker sequence is the mutated BvALS113 sequence carrying in its sequence a mutation at amino acid 113 position from Alanine to Tyrosine.

摘要翻译： 本发明涉及一种用于转化甜菜原生质体的方法，包括以下步骤： - 从分离自甜菜植物的气孔保护细胞获得原生质体， - 用包含感兴趣的核苷酸序列的核酸构建体转化原生质体，以及 选择标记序列， - 以对原生质体的体外培养致死的浓度应用所述原生质体的一种或多种ALS抑制剂的体外培养，以及从具有整合核酸的存活的原生质体再生甜菜植物 构建体，其包含感兴趣的序列和选择标记序列，其中选择标记序列是突变的BvALS113序列，其在氨基酸113位从氨基酸到酪氨酸的突变。

公开(公告)号：WO2015185701A1

公开(公告)日：2015-12-10

申请号：PCT/EP2015/062523

申请日：2015-06-04

申请人： BAYER CROPSCIENCE NV

发明人： DENOLF, Peter ,  LAGA, Benjamin ,  DE BODT, Stefanie ,  CAESTECKER, Evelyne

IPC分类号： C12N15/82 ,  A01H5/10

CPC分类号： C12N15/8222 ,  A01H5/10 ,  C12N15/823 ,  C12N15/8261 ,  C12N15/8271 ,  C12N15/8279 ,  Y02A40/146

摘要： The present invention relates to Brassica sequences comprising pod-preferential promoter activity. Provided are recombinant genes comprising the pod-preferential promoter operably linked to a heterologous nucleic acid sequence, and cells and plants comprising the recombinant gene. The promoters can be used to alter gene expression specifically in the pods and to alter seed yield, seed quality, abiotic stress tolerance, or biotic stress tolerance.

摘要翻译： 本发明涉及包含荚果优先启动子活性的芸苔属序列。 提供了包含可操作地连接到异源核酸序列的荚果优先启动子的重组基因，以及包含重组基因的细胞和植物。 启动子可用于特异性改变荚果中的基因表达并改变种子产量，种子质量，非生物胁迫耐受性或生物胁迫耐受性。