公开(公告)号：WO2016087937A2

公开(公告)日：2016-06-09

申请号：PCT/IB2015/002431

申请日：2015-12-04

Applicant: SMARTZYME INNOVATION LTD.

Inventor： GUTTMAN, Chen, Haim ,  BARAM, David ,  GOFBERG, Itamar, Oz ,  OMER, Dotan

IPC: C12Q1/54 ,  C12N9/06

CPC classification number: C12N9/0006 ,  C12Q1/54 ,  C12Y101/01047 ,  C12Y101/9901

Abstract: In some embodiments, the present invention provides a protein comprising amino acids in the following sequence L(X) n=14 X 3 (X) n=1 X 1 (X) n=1 E(X) n=4 P(X) n=1 NR(X) n=3 S(X) n=4 D(X) n=2 G(X) n=7 Y(X) n=4 Y (X) n=32-34 X 2 , wherein each X independently represents any naturally occurring amino acid residue and n indicates the number of amino acid residues represented by the respective paranthetical at that position, wherein: a) X1 is selected from the group consisting of S, C, T, M, V, Y, N, P, L, G, Q, A, I, D, W, H, or E, wherein if X 1 is L, H or V, then X 3 is D; and/or b) X 2 is selected from the group consisting of H, L, S or V. In some embodiments, the present invention also provides a protein comprising amino acids in the sequence set forth by SEQ ID NO: 38 or SEQ ID NO: 39, except that: the amino acid at position 406 is an amino acid other than F; and/or the amino acid at position 474 is an amino acid other than N.

Abstract translation: 在一些实施方案中，本发明提供包含以下序列中的氨基酸的蛋白质L（X）n = 14X3（X）n = 1X1（X）n = 1E（X）n = 4P（X）n = 1NR ）n = 3S（X）n = 4D（X）n = 2G（X）n = 7Y（X）n = 4Y（X）n = 32-34X2，其中每个X独立地表示任何天然存在的氨基酸残基，n 表示在该位置由相应的表示的氨基酸残基的数目，其中：a）X1选自S，C，T，M，V，Y，N，P，L，G，Q， A，I，D，W，H或E，其中如果X1是L，H或V，则X3是D; 和/或b）X 2选自H，L，S或V.在一些实施方案中，本发明还提供了包含SEQ ID NO：38或SEQ ID NO所示序列中的氨基酸的蛋白质 ：39，除了：406位氨基酸是除F以外的氨基酸; 和/或位置474处的氨基酸是除N以外的氨基酸。