公开(公告)号：WO2010090857A3

公开(公告)日：2010-11-25

申请号：PCT/US2010021589

申请日：2010-01-21

Applicant: VERTEX PHARMA ,  KWONG ANN D ,  FRANTZ JAMES DANIEL ,  BARTELS DOUGLAS J ,  LIN CHAO ,  SHAMES BENJAMIN ,  SEEPERSAUD SHEILA ,  LIPPKE JUDITH A ,  KIEFFER TARA L ,  ZHOU YI ,  ZHANG EILEEN Z ,  SULLIVAN JAMES C

Inventor： KWONG ANN D ,  FRANTZ JAMES DANIEL ,  BARTELS DOUGLAS J ,  LIN CHAO ,  SHAMES BENJAMIN ,  SEEPERSAUD SHEILA ,  LIPPKE JUDITH A ,  KIEFFER TARA L ,  ZHOU YI ,  ZHANG EILEEN Z ,  SULLIVAN JAMES C

IPC: C12Q1/68

CPC classification number: C12Q1/707 ,  C12Q1/6883

Abstract: A method of amplifying an HCV nucleic acid in an HCV infected sample comprises amplifying a segment of a DNA template that is complementary to a genome of HCV RNA from the sample by a two-stage PCR, wherein a first stage PCR employs a first outer primer and a second outer primer, and a second stage PCR employs a first inner primer and a second inner primer. The nucleotide sequence of the first outer primer comprises a nucleotide sequence as set forth in SEQ ID NO: 2; or SEQ ID NO:9, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 9. The nucleotide sequence of the second outer primer comprises a nucleotide sequence set forth in SEQ ID NO: 3 or 4; or a nucleotide sequence as set forth in SEQ ID NO: 10 or 11, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 10 and 11. The nucleotide sequence of the first inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 5; or SEQ ID NO: 12, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 12. The nucleotide sequence of the second inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 6 or 7; or a nucleotide sequence as set forth in SEQ ID NO: 13 or 14, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 13 and 14.

Abstract translation: 在HCV感染样品中扩增HCV核酸的方法包括通过两阶段PCR从样品中扩增与HCV RNA基因组互补的DNA模板的片段，其中第一阶段PCR使用第一外部引物 和第二外引物，第二阶段PCR采用第一内引物和第二内引物。 第一外引物的核苷酸序列包含SEQ ID NO：2所示的核苷酸序列; 或SEQ ID NO：9，其中任选地1,2或3个核苷酸是比SEQ ID NO：9的核苷酸更多的核苷酸。第二外部引物的核苷酸序列包含SEQ ID NO：3或4所示的核苷酸序列; 或如SEQ ID NO：10或11所示的核苷酸序列，其中任选1,2或3个核苷酸是与SEQ ID NO：10和11相同的其它核苷酸。第一内引物的核苷酸序列包含核苷酸序列 如SEQ ID NO：5所示; 或SEQ ID NO：12，其中任选地1,2或3个核苷酸是与SEQ ID NO：12相同的其它核苷酸。第二内引物的核苷酸序列包含SEQ ID NO：6或7所示的核苷酸序列 ; 或如SEQ ID NO：13或14所示的核苷酸序列，其中任选地1,2或3个核苷酸是与SEQ ID NO：13和14相同的其它核苷酸。

公开(公告)号：WO2007145891A3

公开(公告)日：2008-12-18

申请号：PCT/US2007013147

申请日：2007-06-04

Applicant: SULLIVAN JAMES C

Inventor： SULLIVAN JAMES C

IPC: E04B1/16 ,  E01C7/00 ,  E01C9/00 ,  E01C11/22 ,  E01C15/00 ,  E04B2/00 ,  E04D1/36

CPC classification number: E04G15/061 ,  E04G13/06

Abstract: A flow-through concrete edge forming system (10) utilizes a body member (12) that has perforations (20) thereon. The body member (12) is placed at an elevation transition area of a monolithic slab (S) as an edge forming form. Once the slab (S) is poured, a parge coating (P) is placed on the outer surface of the body member (12) such that the parge coating (P) either binds to the outer surface or penetrates the body member (12) through a series of perforations on the body member (12) in order for the parge coating (P) to bind with the concrete of the slab (S) which has yet to set thereby producing a clean and solid finish of the outer surface of the slab (S) at the transition area. The body member ( 12) may have either a rectangular profile (1 12), a keyhole profile (214) or may have the perforations (20) on a series of inwardly directed deformations (18).

Abstract translation: 流通式混凝土边缘形成系统（10）利用其上具有穿孔（20）的主体构件（12）。 主体构件（12）作为边缘形成形式放置在单片板（S）的仰角过渡区域。 一旦浇注了板坯（S），则在本体件（12）的外表面上放置一个涂层（P），使得该涂层涂层（P）或者结合外表面或者穿透本体件（12） 通过在本体构件（12）上的一系列穿孔，以便使涂层（P）与尚未固化的板坯（S）的混凝土结合，从而产生清洁和坚固的外表面 板坯（S）在过渡区域。 主体构件（12）可以具有矩形轮廓（112），锁眼轮廓（214）或者可以在一系列向内指向的变形（18）上具有穿孔（20）。

公开(公告)号：WO2010090857A8

公开(公告)日：2012-06-28

申请号：PCT/US2010021589

申请日：2010-01-21

Applicant: VERTEX PHARMA ,  KWONG ANN D ,  FRANTZ JAMES DANIEL ,  BARTELS DOUGLAS J ,  LIN CHAO ,  SHAMES BENJAMIN ,  SEEPERSAUD SHEILA ,  LIPPKE JUDITH A ,  KIEFFER TARA L ,  ZHOU YI ,  ZHANG EILEEN Z ,  SULLIVAN JAMES C

Inventor： KWONG ANN D ,  FRANTZ JAMES DANIEL ,  BARTELS DOUGLAS J ,  LIN CHAO ,  SHAMES BENJAMIN ,  SEEPERSAUD SHEILA ,  LIPPKE JUDITH A ,  KIEFFER TARA L ,  ZHOU YI ,  ZHANG EILEEN Z ,  SULLIVAN JAMES C

IPC: C12Q1/68

CPC classification number: C12Q1/707 ,  C12Q1/6883

Abstract: A method of amplifying an HCV nucleic acid in an HCV infected sample comprises amplifying a segment of a DNA template that is complementary to a genome of HCV RNA from the sample by a two-stage PCR, wherein a first stage PCR employs a first outer primer and a second outer primer, and a second stage PCR employs a first inner primer and a second inner primer. The nucleotide sequence of the first outer primer comprises a nucleotide sequence as set forth in SEQ ID NO: 2; or SEQ ID NO:9, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 9. The nucleotide sequence of the second outer primer comprises a nucleotide sequence set forth in SEQ ID NO: 3 or 4; or a nucleotide sequence as set forth in SEQ ID NO: 10 or 11, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 10 and 11. The nucleotide sequence of the first inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 5; or SEQ ID NO: 12, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 12. The nucleotide sequence of the second inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 6 or 7; or a nucleotide sequence as set forth in SEQ ID NO: 13 or 14, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 13 and 14.

Abstract translation: 在HCV感染样品中扩增HCV核酸的方法包括通过两阶段PCR从样品中扩增与HCV RNA基因组互补的DNA模板的片段，其中第一阶段PCR使用第一外部引物 和第二外引物，第二阶段PCR采用第一内引物和第二内引物。 第一外引物的核苷酸序列包含SEQ ID NO：2所示的核苷酸序列; 或SEQ ID NO：9，其中任选地1,2或3个核苷酸是比SEQ ID NO：9的核苷酸更多的核苷酸。第二外部引物的核苷酸序列包含SEQ ID NO：3或4所示的核苷酸序列; 或如SEQ ID NO：10或11所示的核苷酸序列，其中任选1,2或3个核苷酸是与SEQ ID NO：10和11相同的其它核苷酸。第一内引物的核苷酸序列包含核苷酸序列 如SEQ ID NO：5所示; 或SEQ ID NO：12，其中任选地1,2或3个核苷酸是与SEQ ID NO：12相同的其它核苷酸。第二内引物的核苷酸序列包含SEQ ID NO：6或7所示的核苷酸序列 ; 或如SEQ ID NO：13或14所示的核苷酸序列，其中任选地1,2或3个核苷酸是与SEQ ID NO：13和14相同的其它核苷酸。