公开(公告)号：WO2021046506A1

公开(公告)日：2021-03-11

申请号：PCT/US2020/049645

申请日：2020-09-08

Applicant: TELEFLEX MEDICAL INCORPORATED

Inventor： VICTOR, John C. ,  SIM, Kristine Marie ,  ADDAE-MENSAH, Kweku Amissah ,  DENLINGER, Rodney Wilmer ,  WAGNER, Victoria E. ,  ULRICH, Robert

IPC: B01L3/00 ,  C12Q1/6806 ,  C12Q1/6888

Abstract: An infection detection system includes a sampling device, a meter, a sample processor, and an analytical instrument. The sampling device is configured to collect a whole blood sample containing a pathogen target. The meter is configured to be removably connected to a portion of the sampling device to meter a predetermined amount of the whole blood sample. The sample processor includes a NASBA fluidic network configured to be in fluid communication with the meter to receive the whole blood sample. The NASBA fluidic network includes an enzyme, a primer, and a beacon. The enzyme and a primer are for amplifying a predetermined genetic sequence in the pathogen target. The beacon is configured to attach to the predetermined genetic sequence in the pathogen target.

公开(公告)号：WO2021046504A1

公开(公告)日：2021-03-11

申请号：PCT/US2020/049642

申请日：2020-09-08

Applicant: TELEFLEX MEDICAL INCORPORATED

Inventor： VICTOR, John C. ,  SIM, Kristine Marie ,  ADDAE-MENSAH, Kweku Amissah ,  DENLINGER, Rodney Wilmer ,  WAGNER, Victoria E. ,  ULRICH, Robert

IPC: B01L3/00 ,  C12Q1/6806 ,  C12Q1/6888

Abstract: An infection detection system includes a sampling device, a lysing chamber, a filter, a meter, a NASBA fluidic network, and an instrument. The sampling device is configured to contain a whole blood sample containing a pathogen target. The lysing chamber is configured to be in fluid communication with the sampling device to receive the whole blood sample. The lysing chamber is configured to lyse the whole blood sample into a lysate. The filter is configured to be in fluid communication with the lysing chamber and to filter the lysate into a filter lysate. The meter is configured to be in fluid communication with the filter and configured to meter a predetermined amount of filtered lysate from the filtered lysate. The NASBA fluidic network is configured to be in fluid communication with the meter to receive the predetermined amount of filtered lysate.