公开(公告)号：WO2021046504A1

公开(公告)日：2021-03-11

申请号：PCT/US2020/049642

申请日：2020-09-08

Applicant: TELEFLEX MEDICAL INCORPORATED

Inventor： VICTOR, John C. ,  SIM, Kristine Marie ,  ADDAE-MENSAH, Kweku Amissah ,  DENLINGER, Rodney Wilmer ,  WAGNER, Victoria E. ,  ULRICH, Robert

IPC: B01L3/00 ,  C12Q1/6806 ,  C12Q1/6888

Abstract: An infection detection system includes a sampling device, a lysing chamber, a filter, a meter, a NASBA fluidic network, and an instrument. The sampling device is configured to contain a whole blood sample containing a pathogen target. The lysing chamber is configured to be in fluid communication with the sampling device to receive the whole blood sample. The lysing chamber is configured to lyse the whole blood sample into a lysate. The filter is configured to be in fluid communication with the lysing chamber and to filter the lysate into a filter lysate. The meter is configured to be in fluid communication with the filter and configured to meter a predetermined amount of filtered lysate from the filtered lysate. The NASBA fluidic network is configured to be in fluid communication with the meter to receive the predetermined amount of filtered lysate.

公开(公告)号：WO2021046336A1

公开(公告)日：2021-03-11

申请号：PCT/US2020/049384

申请日：2020-09-04

Applicant: TELEFLEX MEDICAL INCORPORATED

Inventor： ULRICH, Robert ,  WAGNER, Victoria E. ,  MCDANIEL, Lauren ,  PAUL, John

IPC: C12Q1/689

Abstract: In a method for detecting the presence of pathogenic organisms and/or antibiotic resistance genes in a biological sample, the biological sample is lysed to form a lysate and an amplified lysate is generated by performing a nucleic acid sequence-based (NASBA) amplification for a target nucleic acid sequence in the lysate. The NASBA amplification is performed in the presence of: a forward primer; a reverse primer; and a molecular beacon including a fluorophore. The amplified lysate is exposed to an excitation source and a fluorescence of the fluorophore is detected in the amplified lysate exposed to the excitation source. It is determined whether the pathogenic organisms and/or antibiotic resistance genes are present in the biological sample in response to detecting the fluorescence of the fluorophore.

公开(公告)号：WO2021046506A1

公开(公告)日：2021-03-11

申请号：PCT/US2020/049645

申请日：2020-09-08

Applicant: TELEFLEX MEDICAL INCORPORATED

Inventor： VICTOR, John C. ,  SIM, Kristine Marie ,  ADDAE-MENSAH, Kweku Amissah ,  DENLINGER, Rodney Wilmer ,  WAGNER, Victoria E. ,  ULRICH, Robert

IPC: B01L3/00 ,  C12Q1/6806 ,  C12Q1/6888

Abstract: An infection detection system includes a sampling device, a meter, a sample processor, and an analytical instrument. The sampling device is configured to collect a whole blood sample containing a pathogen target. The meter is configured to be removably connected to a portion of the sampling device to meter a predetermined amount of the whole blood sample. The sample processor includes a NASBA fluidic network configured to be in fluid communication with the meter to receive the whole blood sample. The NASBA fluidic network includes an enzyme, a primer, and a beacon. The enzyme and a primer are for amplifying a predetermined genetic sequence in the pathogen target. The beacon is configured to attach to the predetermined genetic sequence in the pathogen target.

公开(公告)号：WO2021046343A1

公开(公告)日：2021-03-11

申请号：PCT/US2020/049394

申请日：2020-09-04

Applicant: TELEFLEX MEDICAL INCORPORATED

Inventor： ULRICH, Robert ,  WAGNER, Victoria E. ,  MCDANIEL, Lauren

IPC: C12Q1/04 ,  C12Q1/24 ,  G01N33/543

Abstract: A lysis solution for lysing microorganisms in a biological sample includes 2 mM to 16 mM of a Quanidinium thiocyanate/guanidine thiocyanate, 20 µM to 160 µM of a Tris HCL, pH 8.5, 6 µM to 48 µM of a Magnesium chloride, 35 µM to 280 µM of a Potassium chloride, and 0.1% v/v to 1.0% v/v of an octylphenoxypolyethoxyethanol.