公开(公告)号：WO2016183023A1

公开(公告)日：2016-11-17

申请号：PCT/US2016/031506

申请日：2016-05-09

Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY

Inventor： SMOLKE, Christina D. ,  GALANIE, Stephanie ,  TRENCHARD, Isis ,  THODEY, Catherine ,  LI, Yanran

IPC: C12N1/19 ,  C12N15/52 ,  C12N15/53

CPC classification number: C12P17/12 ,  C12N9/0006 ,  C12N9/0071 ,  C12N15/52 ,  C12N15/62 ,  C12N2800/22 ,  C12P41/002 ,  C12Y101/01247

Abstract: A method of epimerizing an ( S )-1-benzylisoquinoline alkaloid to an ( R )-1 -benzylisoquinoline alkaloid is provided. The method comprises contacting the ( S )-1 -benzylisoquinoline alkaloid with at least one enzyme. Contacting the ( S )-1 -benzylisoquinoline alkaloid with the at least one enzyme converts the (S)-1 - benzylisoquinoline alkaloid to an ( R )-1 -benzylisoquinoline alkaloid.

Abstract translation: 提供了将（S）-1-苄基异喹啉生物碱差向异构化为（R）-1-苄基异喹啉生物碱的方法。 该方法包括使（S）-1-苄基异喹啉生物碱与至少一种酶接触。 （S）-1-苄基异喹啉生物碱与至少一种酶接触将（S）-1-苄基异喹啉生物碱转化为（R）-1-苄基异喹啉生物碱。

公开(公告)号：WO2016081371A1

公开(公告)日：2016-05-26

申请号：PCT/US2015/060891

申请日：2015-11-16

Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY

Inventor： SMOLKE, Christina D. ,  LI, Yanran

IPC: C12P13/16

CPC classification number: C12P17/188 ,  C07D273/01 ,  C07D515/02 ,  C07D515/14 ,  C07D515/22 ,  C12N1/02 ,  C12N1/14 ,  C12N9/1003 ,  C12N2330/50 ,  C12P13/16

Abstract: Engineered non-plant cells that produce a benzylisoquinoline alkaloid product that is a derivative of canadine along a metabolic pathway that converts canadine, or an analog of canadine, to a noscapinoid product are provided. Methods of culturing engineered non-plant cells that produce a noscapinoid product and pharmaceutical compositions are also provided.

Abstract translation: 提供了产生苄基异喹啉生物碱产物的工程非植物细胞，其是沿着代谢途径的加拿大的衍生物，其将加拿大或加拿大的类似物转化成硫代西洛定产物。 还提供了培养产生硫代西洛定产物的工程非植物细胞和药物组合物的方法。