公开(公告)号：WO2016081371A1

公开(公告)日：2016-05-26

申请号：PCT/US2015/060891

申请日：2015-11-16

Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY

Inventor： SMOLKE, Christina D. ,  LI, Yanran

IPC: C12P13/16

CPC classification number: C12P17/188 ,  C07D273/01 ,  C07D515/02 ,  C07D515/14 ,  C07D515/22 ,  C12N1/02 ,  C12N1/14 ,  C12N9/1003 ,  C12N2330/50 ,  C12P13/16

Abstract: Engineered non-plant cells that produce a benzylisoquinoline alkaloid product that is a derivative of canadine along a metabolic pathway that converts canadine, or an analog of canadine, to a noscapinoid product are provided. Methods of culturing engineered non-plant cells that produce a noscapinoid product and pharmaceutical compositions are also provided.

Abstract translation: 提供了产生苄基异喹啉生物碱产物的工程非植物细胞，其是沿着代谢途径的加拿大的衍生物，其将加拿大或加拿大的类似物转化成硫代西洛定产物。 还提供了培养产生硫代西洛定产物的工程非植物细胞和药物组合物的方法。

公开(公告)号：WO1996038581A1

公开(公告)日：1996-12-05

申请号：PCT/US1996007845

申请日：1996-05-28

Applicant: THE GOVERNMENT OF THE UNITED STATES OF AMERICA, represented by THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES

Inventor： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, represented by THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES ,  BARRY, Clifton, E., III ,  YUAN, Ying

IPC: C12Q01/02

CPC classification number: G01N33/573 ,  C07K14/35 ,  C12N9/1003 ,  C12Q1/025 ,  C12Q1/48

Abstract: DNA and protein compositions useful for both diagnosis and design of therapeutics for treatment of tuberculosis and other mycobacterial infections. More specifically, the present invention relates to DNA and protein compositions which are responsible for the cyclopropanation of mycolic acids in M. tuberculosis and other pathogenic forms of mycobacterium.

Abstract translation: 用于诊断和设计用于治疗结核病和其他分枝杆菌感染的治疗剂的DNA和蛋白质组合物。 更具体地，本发明涉及负责结核分枝杆菌中的霉菌酸和分枝杆菌的其他致病形式的环丙烷化的DNA和蛋白质组合物。

公开(公告)号：WO2013050024A2

公开(公告)日：2013-04-11

申请号：PCT/DE2012/000974

申请日：2012-10-04

Applicant: KWS SAAT AG ,  STAHL, Dietmar ,  TEMME, Nora ,  SCHÄFER, Wilhelm ,  STÄRKEL, Cornelia

Inventor： STAHL, Dietmar ,  TEMME, Nora ,  SCHÄFER, Wilhelm ,  STÄRKEL, Cornelia

IPC: C12N9/10 ,  C12N15/82 ,  C12N9/00 ,  C07K14/37 ,  C12N15/80 ,  C12N15/113

CPC classification number: C12N9/0004 ,  C12N9/1003 ,  C12N9/1025 ,  C12N15/1137 ,  C12N15/8218 ,  C12N15/8282 ,  C12N2310/14

Abstract: Die Erfindung betrifft eine transgene Pflanze der Art Beta vulgaris, in deren Genom eine Nukleinsäure stabil integriert wurde, von der in der Pflanze RNA transkribiert wird, wobei gebildete RNA im Falle eines Befalls der Pflanze mit einem Pilz der Gattung Cercospora von diesem aufgenommen werden kann, so dass die Cercosporin-Biosynthese in dem Pilz derart beeinträchtigt wird, dass der Pilz in seiner biotrophen Wachstumsphase gehemmt wird und die Pflanze im Vergleich zu einer Kontrollpflanze eine gesteigerte Resistenz gegenüber dem Pilz aufweist.

Abstract translation: 本发明涉及的物种甜菜，核酸被稳定地整合到基因组中，从植物RNA内转录的转基因植物，形成的RNA可以通过该被接收时，在植物的侵染的情况下与该属的尾孢真菌， 使得在真菌cercosporin生物合成以这样的方式使真菌在其生长期活体营养抑制，相比于对照植物对真菌的抗性增加具有所述植物的影响。

公开(公告)号：WO2010091353A2

公开(公告)日：2010-08-12

申请号：PCT/US2010/023485

申请日：2010-02-08

Applicant: UNIVERSITY OF TENNESSEE RESEARCH FOUNDATION ,  CHEN, Feng ,  ZHAO, Nan ,  ARMEL, Gregory, Russell

Inventor： CHEN, Feng ,  ZHAO, Nan ,  ARMEL, Gregory, Russell

IPC: A01H5/00 ,  C12N15/54 ,  C12N15/82

CPC classification number: C12N9/1007 ,  A01N39/02 ,  A01N39/04 ,  C12N9/1003 ,  C12N15/8274

Abstract: The subject invention provides novel polynucleotides and polypeptides encoding a methyltransferase. The subject invention provides novel plants that express the methyltrasferase disclosed herein and are resistant to auxin-based herbicides. The subject invention also provides transgenic plants have been transformed with one or more other herbicide resistance genes such that the plants are resistant to the application of auxin-based herbicides and one or more other herbicides.

Abstract translation: 本发明提供编码甲基转移酶的新型多核苷酸和多肽。 本发明提供了表达本文公开的甲基转移酶且对生长素基除草剂具有抗性的新型植物。 本发明还提供了用一种或多种其他除草剂抗性基因转化的转基因植物，使得植物对施用生长素基除草剂和一种或多种其他除草剂具有抗性。

公开(公告)号：WO2004005527A1

公开(公告)日：2004-01-15

申请号：PCT/US2002/021336

申请日：2002-07-03

Applicant: BASF AKTIENGESELLSCHAFT ,  YOCUM, R., Rogers ,  PATTERSON, Thomas, A. ,  PERO, Janice, G. ,  HERMANN, Theron

Inventor： YOCUM, R., Rogers ,  PATTERSON, Thomas, A. ,  PERO, Janice, G. ,  HERMANN, Theron

IPC: C12P13/04

CPC classification number: C07H21/04 ,  C12N9/0006 ,  C12N9/1003 ,  C12N9/1014 ,  C12N15/52 ,  C12P13/02

Abstract: The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also featured. Also featured are compositions produced by such microorganisms.

Abstract translation: 本发明的特征在于利用具有改良的泛酸生物合成酶活性并具有改性的亚甲基四氢叶酸（MTF）生物合成酶活性的微生物增强生产泛酸盐和泛酸盐的改进方法。 特别地，本发明的特征在于通过增加对其合成直接或间接有贡献的酶或底物来增加关键中间体酮托品酸的水平来增加所需产物的产生的方法。 还具有重组微生物和培养条件。 还有这些微生物产生的组合物。

公开(公告)号：WO2013050024A3

公开(公告)日：2013-07-11

申请号：PCT/DE2012000974

申请日：2012-10-04

Applicant: KWS SAAT AG ,  STAHL DIETMAR ,  TEMME NORA ,  SCHAEFER WILHELM ,  STAERKEL CORNELIA

Inventor： STAHL DIETMAR ,  TEMME NORA ,  SCHAEFER WILHELM ,  STAERKEL CORNELIA

IPC: C12N9/10 ,  C07K14/37 ,  C12N9/00 ,  C12N15/113 ,  C12N15/80 ,  C12N15/82

CPC classification number: C12N9/0004 ,  C12N9/1003 ,  C12N9/1025 ,  C12N15/1137 ,  C12N15/8218 ,  C12N15/8282 ,  C12N2310/14

Abstract: The invention relates to a transgenic plant of the species Beta vulgaris, into the genome of which a nucleic acid was stably integrated. From said nucleic acid RNA is transcribed in the plant, wherein RNA formed in the event of an infestation of the plant with a fungus of the genus Cercospora can be taken up by said RNA so that the Cercosporin biosynthesis in the fungus is impaired in such a manner that biotrophic growth phase of the fungus is inhibited and the plant has an enhanced resistance to the fungus compared with a control plant.

Abstract translation: 本发明涉及的物种甜菜，核酸被稳定地整合到基因组中，从植物RNA内转录的转基因植物，形成的RNA可以通过该被接收时，在植物的侵染的情况下与该属的尾孢真菌， 使得在真菌cercosporin生物合成以这样的方式使真菌在其生长期活体营养抑制，相比于对照植物对真菌的抗性增加具有所述植物的影响。

公开(公告)号：WO2010091353A3

公开(公告)日：2010-11-25

申请号：PCT/US2010023485

申请日：2010-02-08

Applicant: UNIV TENNESSEE RES FOUNDATION ,  CHEN FENG ,  ZHAO NAN ,  ARMEL GREGORY RUSSELL

Inventor： CHEN FENG ,  ZHAO NAN ,  ARMEL GREGORY RUSSELL

IPC: A01H5/00 ,  C12N15/54 ,  C12N15/82

CPC classification number: C12N9/1007 ,  A01N39/02 ,  A01N39/04 ,  C12N9/1003 ,  C12N15/8274

Abstract: The subject invention provides novel polynucleotides and polypeptides encoding a methyltransferase. The subject invention provides novel plants that express the methyltrasferase disclosed herein and are resistant to auxin-based herbicides. The subject invention also provides transgenic plants that have been transformed with one or more other herbicide resistance genes such that the plants are resistant to the application of auxin-based herbicides and one or more other herbicides.

Abstract translation: 本发明提供了编码甲基转移酶的新型多核苷酸和多肽。 本发明提供了表达本文公开的甲基转移酶并且对生长素基除草剂具有抗性的新型植物。 本发明还提供已经用一种或多种其它除草剂抗性基因转化的转基因植物，使得植物对施用生长素基除草剂和一种或多种其它除草剂具有抗性。

公开(公告)号：WO2007035693A9

公开(公告)日：2007-07-05

申请号：PCT/US2006036401

申请日：2006-09-19

Applicant: HARVARD COLLEGE ,  CLARDY JON ,  BRADY SEAN F

Inventor： CLARDY JON ,  BRADY SEAN F

IPC: C12N15/43 ,  C12N9/10 ,  C12N9/88 ,  C12N15/60 ,  C12P17/10

CPC classification number: C12N9/1003 ,  C07C291/10 ,  C07D209/18 ,  C07D239/56 ,  C07D417/04 ,  C12N9/88 ,  C12P13/002 ,  C12P17/10 ,  C40B30/06 ,  C40B40/02 ,  C40B50/06

Abstract: Novel isonitrile biosynthetic enzymes isnA and isnB which together possess a catalytic activity of converting an amine-containing compound to an isonitrile-containing compound are described. The genes that encode such enzymes, recombinant vectors containing one or both of such genes, a host cell transformed with said vector, methods for the production and isolation of the enzymes of the invention are also described. Methods for the identification of bioactive, isonitrile-containing natural products derived from the biosynthetic activity of the enzymes of the invention, and methods for synthetically producing isonitrile-containing compounds from the isolated enzymes of the invention are also described.

Abstract translation: 描述了新颖的异腈生物合成酶isnA和isnB，它们一起具有将含胺化合物转化为含异腈化合物的催化活性。 编码这些酶的基因，含有一个或两个此类基因的重组载体，用所述载体转化的宿主细胞，用于生产和分离本发明酶的方法也被描述。 还描述了鉴定源自本发明酶的生物合成活性的含生物活性异腈的天然产物的方法，以及从本发明分离的酶合成生产含异腈化合物的方法。

公开(公告)号：WO2003078574A2

公开(公告)日：2003-09-25

申请号：PCT/US2003/007242

申请日：2003-03-07

Applicant: LIPOMICS TECHNOLOGIES, INC. ,  WATKINS, Steven, M. ,  BAILLIE, Rebecca, A.

Inventor： WATKINS, Steven, M. ,  BAILLIE, Rebecca, A.

IPC: C12N

CPC classification number: G01N33/92 ,  A61K31/00 ,  A61K31/4415 ,  A61K31/455 ,  A61K31/51 ,  A61K31/519 ,  A61K31/7076 ,  A61K38/27 ,  C12N9/1003 ,  C12Y201/01017 ,  G01N33/57484 ,  G01N2800/52

Abstract: The present invention provides metabolic markers and targets, especially PEMT activity and metabolic markers and components associated therewith useful for diagnosing and treating various disease conditions.

Abstract translation: 本发明提供代谢标志物和靶标，特别是PEMT活性和与其相关的代谢标志物和组分，其可用于诊断和治疗各种疾病状况。

公开(公告)号：WO2007035693A3

公开(公告)日：2007-08-16

申请号：PCT/US2006036401

申请日：2006-09-19

Applicant: HARVARD COLLEGE ,  CLARDY JON ,  BRADY SEAN F

Inventor： CLARDY JON ,  BRADY SEAN F

IPC: C12N15/54 ,  C12N9/10 ,  C12N9/88 ,  C12N15/60 ,  C12P17/10

CPC classification number: C12N9/1003 ,  C07C291/10 ,  C07D209/18 ,  C07D239/56 ,  C07D417/04 ,  C12N9/88 ,  C12P13/002 ,  C12P17/10 ,  C40B30/06 ,  C40B40/02 ,  C40B50/06

Abstract: Novel isonitrile biosynthetic enzymes isnA and isnB which together possess a catalytic activity of converting an amine-containing compound to an isonitrile-containing compound are described. The genes that encode such enzymes, recombinant vectors containing one or both of such genes, a host cell transformed with said vector, methods for the production and isolation of the enzymes of the invention are also described. Methods for the identification of bioactive, isonitrile-containing natural products derived from the biosynthetic activity of the enzymes of the invention, and methods for synthetically producing isonitrile-containing compounds from the isolated enzymes of the invention are also described.