公开(公告)号：WO2014085713A1

公开(公告)日：2014-06-05

申请号：PCT/US2013/072416

申请日：2013-11-27

Applicant: THE METHODIST HOSPITAL RESEARCH INSTITUTE

Inventor： HU, Ye ,  MA, Xin ,  WU, Hung-Jen ,  FAN, Jia ,  SUN, Tong

IPC: G01N33/543 ,  G01N33/552

CPC classification number: G01N33/5695 ,  G01N33/552 ,  G01N33/569 ,  G01N2333/35 ,  G01N2469/10

Abstract: Improved methods for detecting active tuberculosis are disclosed. A method comprises enriching at least one M. tuberculosis- specific biomolecule from a sample by contacting the sample with a nanoporous film; and detecting the presence of the M. tuberculosis-specific biomolecule or fragment(s) thereof. The method may further comprise digesting the enriched M. tuberculosis -specific biomolecule with an enzyme to produce a digestion product comprising at least one fragment of the M. tuberculosis -specific biomolecule. Improved sensitivity and speed achieved.

Abstract translation: 公开了用于检测活动性结核病的改进方法。 一种方法包括通过使样品与纳米孔膜接触来从样品中富集至少一种结核分枝杆菌特异性生物分子; 并检测结核分枝杆菌特异性生物分子或其片段的存在。 该方法还可以包括用酶消化富集的结核分枝杆菌特异性生物分子，以产生包含结核分枝杆菌特异性生物分子的至少一个片段的消化产物。 提高灵敏度和速度。