公开(公告)号：WO2016019324A2

公开(公告)日：2016-02-04

申请号：PCT/US2015/043266

申请日：2015-07-31

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： OZCAN, Aydogan ,  GREENBAUM, Alon ,  ZHANG, Yibo ,  FEIZI, Alborz ,  LUO, Wei

IPC: G03H1/04 ,  G03H1/00

CPC classification number: G03H1/0005 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1475 ,  G01N2015/1006 ,  G01N2015/144 ,  G01N2015/1445 ,  G01N2015/1454 ,  G03H1/0443 ,  G03H1/0866 ,  G03H1/0891 ,  G03H2001/005 ,  G03H2001/0447 ,  G03H2001/0454 ,  G03H2001/046 ,  G03H2001/0816 ,  G03H2001/0883 ,  G03H2001/2655 ,  G03H2210/42 ,  G03H2226/02 ,  G03H2227/03 ,  H04N5/2256 ,  H04N5/23238

Abstract: A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.

Abstract translation: 用于样品或样品的透镜自由成像的方法使用多重迭代相位检索和旋转场转换来对具有临床上可比较的图像质量的病理样本进行宽的FOV成像到基于台式透镜的显微镜。 传输强度（TIE）方程的解决方案用作相位恢复过程中的初始猜测，以加速图像恢复过程。 全息重构的图像可以在物体FOV（图像捕获之后）中的任何深度进行数字聚焦，而不需要任何焦点调整，并且还可以对由样品和传感器平面之间的不受控制的倾斜和高度变化引起的伪影进行数字校正。 在替代实施例中，使用合成孔径方法进行多角度迭代相位检索以对病理样本进行宽FOV成像并增加图像的有效数值孔径。

公开(公告)号：WO2017196995A1

公开(公告)日：2017-11-16

申请号：PCT/US2017/031984

申请日：2017-05-10

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： OZCAN, Aydogan ,  WU, Yichen ,  ZHANG, Yibo ,  LUO, Wei

IPC: G03H1/04

Abstract: A method of generating a color image of a sample includes obtaining a plurality of low resolution holographic images of the sample using a color image sensor, the sample illuminated simultaneously by light from three or more distinct colors, wherein the illuminated sample casts sample holograms on the image sensor and wherein the plurality of low resolution holographic images are obtained by relative x, y, and z directional shifts between sample holograms and the image sensor. Pixel super-resolved holograms of the sample are generated at each of the three or more distinct colors. De-multiplexed holograms are generated from the pixel super-resolved holograms. Phase information is retrieved from the de-multiplexed holograms using a phase retrieval algorithm to obtain complex holograms. The complex hologram for the three or more distinct colors is digitally combined and back-propagated to a sample plane to generate the color image.

Abstract translation: 产生样本的彩色图像的方法包括使用彩色图像传感器获得样本的多个低分辨率全息图像，样本由来自三种或更多种不同颜色的光同时照射，其中 照射样本在图像传感器上投射样本全息图，并且其中通过样本全息图和图像传感器之间的相对x，y和z方向位移来获得多个低分辨率全息图像。 样品的像素超分辨全息图在三种或更多种不同颜色中的每一种上产生。 解复用全息图由像素超分辨全息图生成。 使用相位检索算法从解复用全息图中检索相位信息以获得复杂的全息图。 用于三种或更多种不同颜色的复合全息图被数字组合并且向后传播到样本平面以生成彩色图像。