公开(公告)号：WO2016100234A1

公开(公告)日：2016-06-23

申请号：PCT/US2015/065624

申请日：2015-12-14

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： DI CARLO, Dino ,  MURRAY, Coleman ,  PAO, Edward ,  TSENG, Peter

IPC分类号： B03C1/02 ,  B03C1/025 ,  B03C1/03 ,  G01N35/00

CPC分类号： C12M47/04 ,  B03C1/03 ,  B03C1/0332 ,  B03C1/247 ,  B03C1/253 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  G01N15/0656 ,  G01N35/0098 ,  G01N2015/0053

摘要： A system is provided for the quantitative magnetic separation of magnetic objects (e.g., particles or cells). The system uses magnetic ratcheting over arrays of ferromagnetic elements having gradient spacing manifested in various pitch zones that are encountered by the magnetic objects as they traverse the array. The system can be used to separate and concentrate magnetic objects based on iron oxide content. For cells, different phenotypes may be separated based, for example, on surface expression of proteins or molecules that are bound to magnetic particles. The system includes a substrate or chip having the array of ferromagnetic elements with increasing lateral pitch and an externally driven magnet device that generates a cycling magnetic field. Magnetic objects with higher IOC separate and equilibrate along the array at larger pitches. The system can be used for the differential sorting of particles and cells of interest.

摘要翻译： 提供了用于磁性物体（例如，颗粒或细胞）的定量磁分离的系统。 该系统在具有梯度间隔的铁磁元件的阵列上使用磁性棘轮，其表现为在磁性物体横穿阵列时遇到的各种间距区域。 该系统可用于基于氧化铁含量分离和集中磁性物体。 对于细胞，可以例如基于与磁性颗粒结合的蛋白质或分子的表面表达来分离不同的表型。 该系统包括具有增加的横向间距的铁磁元件阵列的基板或芯片以及产生循环磁场的外部驱动的磁体装置。 具有较高IOC的磁性物体以更大的间距沿阵列分离和平衡。 该系统可用于感兴趣的颗粒和细胞的差异分选。