公开(公告)号：WO2016100635A1

公开(公告)日：2016-06-23

申请号：PCT/US2015/066321

申请日：2015-12-17

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： COLLINS, Philip, G. ,  WEISS, Gregory, A. ,  CHOI, Yongki ,  OLSEN, Tivoli

IPC: C12Q1/48 ,  C12Q1/68 ,  G01N30/02 ,  G01N27/00 ,  B05D5/12

CPC classification number: C12Q1/485 ,  C12Q1/48 ,  C12Y207/07007 ,  G01N27/4146 ,  G01N2333/9126

Abstract: The invention provides methods and compositions for detecting a change in a nucleic acid polymerase conformation involving contacting a nucleic acid polymerase non-covalently attached to a single walled carbon nanotube (SWNT) with a first nucleotide or first nucleotide analog and a template and detecting the conformationally changed nucleic acid polymerase by measuring a first electrical conductance change in the SWNT between the nucleic acid polymerase and the conformationally changed nucleic acid polymerase. The method is useful for sequencing of polynucleotides.

Abstract translation: 本发明提供了用于检测核酸聚合酶构象变化的方法和组合物，其涉及将非共价连接到单壁碳纳米管（SWNT）的核酸聚合酶与第一核苷酸或第一核苷酸类似物和模板接触并检测构象 通过测量核酸聚合酶和构象改变的核酸聚合酶之间的SWNT中的第一导电变化来改变核酸聚合酶。 该方法对多核苷酸的测序是有用的。

公开(公告)号：WO2016100637A1

公开(公告)日：2016-06-23

申请号：PCT/US2015/066324

申请日：2015-12-17

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： COLLINS, Philip, G. ,  WEISS, Gregory, A. ,  CHOI, Yongki ,  OLSEN, Tivoli

IPC: C12Q1/48 ,  C12Q1/68 ,  G01N30/02 ,  G01N27/00 ,  B05D5/12

CPC classification number: C12Q1/485 ,  C12Q1/48 ,  C12Y207/07007 ,  G01N27/4146 ,  G01N2333/9126

Abstract: The invention provides methods and compositions for detecting a change in a nucleic acid polymerase conformation involving contacting a nucleic acid polymerase non-covalently attached to a single walled carbon nanotube (SWNT) with a first nucleotide or first nucleotide analog and a template and detecting the conformationally changed nucleic acid polymerase by measuring a first electrical conductance change in the SWNT between the nucleic acid polymerase and the conformationally changed nucleic acid polymerase. The method is useful for sequencing of polynucleotides.

Abstract translation: 本发明提供了用于检测核酸聚合酶构象变化的方法和组合物，包括将非共价连接于单壁碳纳米管（SWNT）的核酸聚合酶与第一核苷酸或第一核苷酸 类似物和模板，并且通过测量核酸聚合酶和构象改变的核酸聚合酶之间的SWNT中的第一电导变化来检测构象变化的核酸聚合酶。 该方法可用于多核苷酸的测序。