公开(公告)号：WO2018185257A1

公开(公告)日：2018-10-11

申请号：PCT/EP2018/058802

申请日：2018-04-05

Applicant: THE PROVOST, FELLOWS, SCHOLARS AND OTHER MEMBERS OF BOARD OF TRINITY COLLEGE DUBLIN

Inventor： COLEMAN, Jonathan ,  KELLY, Adam ,  HALLAM, Toby ,  DUESBERG, Georg

IPC: G01N27/414 ,  H01L29/00 ,  H01M10/00

CPC classification number: H01L29/00 ,  G01N27/4146 ,  H01G9/2004 ,  H01G9/2031 ,  H01G9/2059 ,  H01G11/04 ,  H01G11/26 ,  H01G11/46 ,  H01G11/58 ,  H01L51/0508 ,  H01M14/005

Abstract: The invention provides a multi-layer device structure comprising an active layer of material; and an insulating layer of material, characterised in that the insulating layer is a porous material (e.g. a nanomaterial) and comprises a liquid electrolyte. The insulating layer contains an electrolyte within its porous interior that allows liquid-like ionic mobility in a solid-like structure. The insulating layer can be used to make a number of devices, such as a transistor, a capacitor, or a solar cell. The invention is particularly suited to the field of printed electronics.

公开(公告)号：WO2017193423A1

公开(公告)日：2017-11-16

申请号：PCT/CN2016/083578

申请日：2016-05-27

Applicant: 江苏大学

Inventor： 徐坤 ,  张西良 ,  耿妙妙 ,  崔守娟 ,  李萍萍 ,  张世庆

IPC: G01N27/30 ,  G01N27/26

CPC classification number: G01N27/302 ,  B82Y5/00 ,  B82Y15/00 ,  B82Y30/00 ,  G01N27/4146 ,  H01L51/0048

Abstract: 一种微纳织构化石墨烯基仿生pH传感器及其制备方法，包括基板（1）、工作电极（4）、参比电极（5）、铜触点A（6）、铜触点B（7）、内部引线A（8）和内部引线B（9）；基板（1）上开有槽A（2）和槽B（3）；工作电极（4）位于槽A（2）内，参比电极（5）位于槽B（3）内；工作电极（4）底部通过铜触点A（6）与内部引线A（8）连接；参比电极（5）顶部通过铜触点B（7）与内部引线B（9）连接；工作电极（4）包括石墨烯基A（401）和敏感电极材料层（402）；敏感电极材料层（402）位于石墨烯基A（401）上层；参比电极（5）包括石墨烯基B（501）和金属材料银层（502）；金属材料银层（502）位于石墨烯基B（501）下层；敏感电极材料层（402）上表面和金属材料银层（502）下表面均开有微凹槽或微凹坑。pH传感器能够快速吸附土壤和栽培基质中的水分，进而吸附氢离子，实现对土壤和栽培基质等非均相体系的pH原位测量。

公开(公告)号：WO2017137086A1

公开(公告)日：2017-08-17

申请号：PCT/EP2016/052941

申请日：2016-02-11

Applicant: UNIVERSITEIT LEIDEN ,  ENVIRONMENTAL MONITORING SYSTEMS (EMS) B.V.

Inventor： VAN DIJKMAN, Thomas, Frederik ,  BOUWMAN, Elisabeth ,  FU, Wangyang ,  SCHNEIDER, Gregory ,  BOERMAN, Jan-Kees

IPC: G01N27/414

CPC classification number: G01N27/4141 ,  G01N27/4146

Abstract: The present invention relates to a device for detection of a gaseous analyte. Specifically, the present invention relates to a device for detection of a gaseous analyte, wherein the device is comprised of a first layer, being a substrate layer, a second layer, being an electrical insulating layer interposed between the first layer and a third layer, being a graphene layer and a fourth layer that is comprised of transition metal complexes, wherein the fourth layer is provided on a top surface of the graphene layer, wherein said transition metal complexes are capable to form a stable complex with said gaseous analyte. Furthermore the device comprises at least two electrodes being in electrical communication with the graphene layer and are capable of measuring a change in conductance of the graphene layer by measuring a change in electric potential between electrodes. Furthermore the present invention relates to methods for the detection of gaseous analyte and the use of the device of present invention to measure the gaseous analyte concentrations.

Abstract translation: 本发明涉及一种用于检测气态分析物的装置。 特别地，本发明涉及一种用于检测气态分析物的装置，其中该装置包括作为衬底层的第一层，作为介于第一层和第三层之间的电绝缘层的第二层， 是石墨烯层和由过渡金属络合物组成的第四层，其中第四层设置在石墨烯层的顶表面上，其中所述过渡金属络合物能够与所述气态分析物形成稳定的络合物。 此外，该装置包括至少两个电极，其与石墨烯层电连通，并且能够通过测量电极之间的电势变化来测量石墨烯层的电导率的变化。 此外，本发明涉及用于检测气态分析物的方法以及本发明的装置用于测量气态分析物浓度的用途。

公开(公告)号：WO2017098517A1

公开(公告)日：2017-06-15

申请号：PCT/IL2016/051319

申请日：2016-12-08

Applicant: RAMOT AT TEL-AVIV UNIVERSITY LTD.

Inventor： PATOLSKY, Fernando ,  KRIVITSKY, Vadim ,  ZVERZHINETSKY, Marina

IPC: B82Y10/00 ,  G01N27/327 ,  G01N27/414 ,  G01N33/53 ,  G01N33/543

CPC classification number: B82Y15/00 ,  G01N27/4146 ,  H01L23/532 ,  H01L29/06 ,  H01L51/0049 ,  H01L51/05 ,  H01L51/0545 ,  H01L51/0558

Abstract: A method of detecting a presence and/or concentration of a marker, e.g ., a marker, in a liquid, e.g. , a liquid, is disclosed. The method comprises: contacting the liquid with a sensor having an immobilized affinity moiety interacting with the marker and being configured to generate a detectable signal responsively to the interaction. The method further comprises washing the liquid off the sensor, and detecting the presence and/or concentration of the marker based on a detectable signal received from the sensor within a time-window beginning a predetermined time period after a beginning time of the washing.

Abstract translation: 检测液体例如液体中标记物（例如标记）的存在和/或浓度的方法 被披露。 该方法包括：使液体与具有与标记相互作用的固定的亲和部分的传感器接触，并配置为响应于相互作用产生可检测的信号。 该方法进一步包括将液体从传感器上清除，并且基于在洗涤开始时间之后的预定时间段内开始的时间窗内从传感器接收的可检测信号来检测标记的存在和/或浓度。 / p>

公开(公告)号：WO2017041056A8

公开(公告)日：2017-04-20

申请号：PCT/US2016050295

申请日：2016-09-02

Applicant: AGILOME INC

Inventor： HOFFMAN PAUL

IPC: G01N27/403

CPC classification number: G01N27/4146

Abstract: This invention concerns Chemically-sensitive Field Effect Transistors (ChemFETs) that are preferably fabricated using semiconductor fabrication methods on a semiconductor wafer, and in preferred embodiments, on top of an integrated circuit structure made using semiconductor fabrication methods. The instant ChemFETs typically comprise a conductive source, a conductive drain, and a channel composed of a one-dimensional (ID) or two-dimensional (2D) transistor nanomaterial, which channel extends from the source to the drain and is fabricated using semiconductor fabrication techniques on top of a wafer. The ChemFET also includes a gate, often the gate voltage is provided through a fluid or solution proximate the ChemFET. Such ChemFETs, preferably configured in independently addressable arrays, may be employed to detect a presence and/or concentration changes of various analyte types in chemical and/or biological samples, including nucleic acid hybridization and/or sequencing reactions.

Abstract translation: 本发明涉及化学敏感场效应晶体管（ChemFET），其优选使用半导体制造方法在半导体晶片上制造，并且在优选实施例中，在使用半导体制造方法制造的集成电路结构的顶部。 本发明的ChemFET典型地包括由一维（ID）或二维（2D）晶体管纳米材料组成的导电源，导电漏极和沟道，该沟道从源极延伸到漏极并且使用半导体制造 技术在晶圆上。 ChemFET还包括一个栅极，栅极电压通常通过靠近ChemFET的流体或溶液提供。 优选配置在独立可寻址阵列中的这种ChemFET可用于检测化学和/或生物样品中各种分析物类型（包括核酸杂交和/或测序反应）的存在和/或浓度变化。

公开(公告)号：WO2016127007A3

公开(公告)日：2016-10-06

申请号：PCT/US2016016664

申请日：2016-02-04

Applicant: HARVARD COLLEGE

Inventor： XIE PING

IPC: G01N33/487

CPC classification number: G01N33/48721 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2300/0645 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2300/12 ,  B01L2400/0421 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N27/4146 ,  G01N27/4163 ,  G01N27/44726 ,  G01N27/4473 ,  G01N27/44791

Abstract: A nanopore sensor (3) is provided, including a nanopore (12) disposed in support structure (14). A fluidic passage (105) is disposed between a first fluidic reservoir (104) and the nanopore (12) to fluidically connect the first fluidic reservoir (104) to the nanopore (12) through the fluidic passage (105). The fluidic passage (105) has a passage length that is greater than the passage width. The fluidic passage (105) is therefore n the form of a micro-channel. A second fluidic reservoir (102) is fluidically connected to the nanopore (12), with the nanopore (12) providing fluidic communication between the fluidic passage (105) the second reservoir (102). Electrodes (13, 15) are connected to impose an electrical potential difference across the nanopore (12). At least one electrical transduction element (7) is disposed in the nanopore sensor (3) with a connection to measure the electrical potential that is local to the fluidic passage (105). The electrical transduction element (7) is provided as a field effect transistor, a single electron transistor or a fluorescent dye sensitive to the electrical potential near the nannopore (12). The ratio of ionic concentrations between the first fluidic and second fluidic reservoir (102, 105) can be adjusted in order to improve electrical potential sensing near the nanopore (12).

Abstract translation: 提供纳米孔传感器（3），其包括设置在支撑结构（14）中的纳米孔（12）。 流体通道（105）设置在第一流体储存器（104）和纳米孔（12）之间，以通过流体通道（105）将第一流体储存器（104）流体连接到纳米孔（12）。 流体通道（105）具有大于通道宽度的通道长度。 流体通道（105）因此是微通道的形式。 第二流体储存器（102）与纳米孔（12）流体连接，纳米孔（12）在第二储存器（102）的流体通道（105）之间提供流体连通。 电极（13,15）被连接以在纳米孔（12）上施加电势差。 至少一个电传导元件（7）通过连接设置在纳米孔传感器（3）中，以测量流体通道（105）局部的电位。 电传导元件（7）被提供为场效应晶体管，单电子晶体管或对纳米孔附近的电势敏感的荧光染料（12）。 可以调节第一流体和第二流体储存器（102,105）之间的离子浓度的比例，以便改进纳米孔（12）附近的电势感测。

公开(公告)号：WO2016145110A1

公开(公告)日：2016-09-15

申请号：PCT/US2016/021606

申请日：2016-03-09

Applicant: EDICO GENOME, INC.

Inventor： HOFFMAN, Paul ,  LERNER, Mitchell ,  ROOYEN, Pieter van

IPC: C12Q1/68 ,  G01N27/414 ,  H01L21/335

CPC classification number: C12Q1/68 ,  B82Y30/00 ,  C12Q1/6874 ,  G01N27/4146 ,  C12Q2535/122 ,  C12Q2563/113 ,  C12Q2563/116 ,  C12Q2563/143 ,  C12Q2563/155 ,  C12Q2565/607

Abstract: Provided herein are devices, systems, and methods of employing the same for the performance of bioinformatics analysis. The apparatuses and methods of the disclosure are directed in part to large scale graphene FET sensors, arrays, and integrated circuits employing the same for analyte measurements. The present GFET sensors, arrays, and integrated circuits may be fabricated using conventional CMOS processing techniques based on improved GFET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense GFET sensor based arrays. Improved fabrication techniques employing graphene as a reaction layer provide for rapid data acquisition from small sensors to large and dense arrays of sensors. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes, including DNA hybridization and/or sequencing reactions.

Abstract translation: 本文提供了使用其进行生物信息学分析的装置，系统和方法。 本公开的装置和方法部分地涉及用于分析物测量的大规模石墨烯FET传感器，阵列和集成电路。 可以使用基于改进的GFET像素和阵列设计的传统CMOS处理技术来制造当前的GFET传感器，阵列和集成电路，所述GFET像素和阵列设计增加了测量灵敏度和精度，并且同时促进了显着小的像素尺寸和基于密集的基于GFET传感器的阵列。 使用石墨烯作为反应层的改进的制造技术提供从小型传感器到大型和致密传感器阵列的快速数据采集。 可以使用这样的阵列来检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化，包括DNA杂交和/或测序反应。

公开(公告)号：WO2016100635A1

公开(公告)日：2016-06-23

申请号：PCT/US2015/066321

申请日：2015-12-17

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： COLLINS, Philip, G. ,  WEISS, Gregory, A. ,  CHOI, Yongki ,  OLSEN, Tivoli

IPC: C12Q1/48 ,  C12Q1/68 ,  G01N30/02 ,  G01N27/00 ,  B05D5/12

CPC classification number: C12Q1/485 ,  C12Q1/48 ,  C12Y207/07007 ,  G01N27/4146 ,  G01N2333/9126

Abstract: The invention provides methods and compositions for detecting a change in a nucleic acid polymerase conformation involving contacting a nucleic acid polymerase non-covalently attached to a single walled carbon nanotube (SWNT) with a first nucleotide or first nucleotide analog and a template and detecting the conformationally changed nucleic acid polymerase by measuring a first electrical conductance change in the SWNT between the nucleic acid polymerase and the conformationally changed nucleic acid polymerase. The method is useful for sequencing of polynucleotides.

Abstract translation: 本发明提供了用于检测核酸聚合酶构象变化的方法和组合物，其涉及将非共价连接到单壁碳纳米管（SWNT）的核酸聚合酶与第一核苷酸或第一核苷酸类似物和模板接触并检测构象 通过测量核酸聚合酶和构象改变的核酸聚合酶之间的SWNT中的第一导电变化来改变核酸聚合酶。 该方法对多核苷酸的测序是有用的。

公开(公告)号：WO2015200853A1

公开(公告)日：2015-12-30

申请号：PCT/US2015/038111

申请日：2015-06-26

Applicant: THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS

Inventor： SALEHI, Amin ,  YASAEI, Poya ,  KUMAR, Bijandra

IPC: B82Y15/00 ,  H01L27/28 ,  H01L29/04

CPC classification number: G01N27/4146 ,  G01N27/414

Abstract: The present invention relates generally to the field of chemical sensing. More specifically, in certain aspects the present invention relates to graphene -based devices and methods useful in chemical sensing. One aspect of the invention is a sensing device including a first graphene grain; a second graphene grain disposed in substantial contact with the first graphene grain, the first graphene grain and the second graphene grain having a grain boundary formed therebetween; a first electrode operatively coupled to the first graphene grain; a second electrode operatively coupled to the second graphene grain; and an electrical measurement system coupled to the first electrode and the second electrode, the electrical measurement system being configured to measure one or more electrical properties of the grain boundary.

Abstract translation: 本发明一般涉及化学感测领域。 更具体地，在某些方面，本发明涉及基于石墨烯的器件和用于化学感测的方法。 本发明的一个方面是包括第一石墨烯晶粒的感测装置; 设置成与第一石墨烯晶粒基本接触的第二石墨烯晶粒，其间形成有晶界的第一石墨烯晶粒和第二石墨烯晶粒; 可操作地耦合到第一石墨烯晶粒的第一电极; 操作地耦合到第二石墨烯晶粒的第二电极; 以及耦合到所述第一电极和所述第二电极的电测量系统，所述电测量系统被配置为测量所述晶界的一个或多个电特性。

公开(公告)号：WO2015162301A2

公开(公告)日：2015-10-29

申请号：PCT/EP2015/059097

申请日：2015-04-27

Applicant: DNA ELECTRONICS LTD

Inventor： TOUMAZOU, Christofer ,  DAVIDSON, David ,  REED, Sam

IPC: C12Q1/68

CPC classification number: C12M1/34 ,  B01L3/5027 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/087 ,  C12M41/48 ,  C12Q1/6825 ,  C12Q1/6844 ,  C12Q1/6874 ,  G01N27/4146 ,  C12Q2537/149 ,  C12Q2565/629 ,  C12Q2565/537 ,  C12Q2565/607

Abstract: A method of analysing nucleic acid using apparatus comprising a reaction chamber and plurality of sensors located in the base of the chamber, with each sensor preferably located within a respective well. The method comprises flowing a fluid containing the nucleic acid or fragments thereof into the reaction chamber. While the chamber is fully or at least partially sealed, amplification of the nucleic acid or said fragments is performed within the chamber using an amplification primer or primers whilst detecting the generation of amplicons using said sensors. Sequencing or hybridisation is then performed on the amplicons, and sequencing or hybridisation is detected using said sensors.

Abstract translation: 一种使用装置分析核酸的方法，所述设备包括反应室和位于所述室的底部中的多个传感器，每个传感器优选地位于相应的孔内。 该方法包括将含有核酸或其片段的流体流入反应室。 当室完全或至少部分密封时，使用扩增引物或引物在室内进行核酸或所述片段的扩增，同时使用所述传感器检测扩增子的产生。 然后对扩增子进行测序或杂交，并使用所述传感器检测测序或杂交。