公开(公告)号：WO2017194924A1

公开(公告)日：2017-11-16

申请号：PCT/GB2017/051282

申请日：2017-05-09

Applicant: THE UNIVERSITY OF BIRMINGHAM

Inventor： CHEN, Frederick ,  FRUMENTO, Guido ,  MOSS, Paul Austin Henry

IPC: C12N5/0783

CPC classification number: C12N5/0636 ,  C12N5/0087 ,  C12N2501/2302 ,  C12N2501/2304 ,  C12N2501/2306 ,  C12N2501/2307 ,  C12N2501/2315 ,  C12N2501/2321 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2510/00

Abstract: Provided is a method of sorting T cells based on expression of CD117. The T cells may comprise a nucleotide sequence encoding a TCR and/or a CAR. Prior to sorting, the cells may have undergone reversion to a naïve phenotype. Also provided is a method of generating T cells having a naïve phenotypewhich comprises culturing central memory and/or effector memory T cells in a culture medium comprising a cytokine. Cell populations and uses of the T cells in therapy are also described.

Abstract translation: 提供了基于CD117的表达对T细胞进行分选的方法。 T细胞可以包含编码TCR和/或CAR的核苷酸序列。 在分选之前，细胞可能已经经历了逆转为无显着表型。 还提供了产生具有表型的T细胞的方法，其包括在包含细胞因子的培养基中培养中枢记忆和/或效应记忆T细胞。 还描述了细胞群体和T细胞在治疗中的用途。