公开(公告)号：WO2014150290A1

公开(公告)日：2014-09-25

申请号：PCT/US2014/022847

申请日：2014-03-10

Applicant: THERANOS, INC.

Inventor： LOO, Alexander ,  HOLMES, Elizabeth, A.

IPC: G01N33/48 ,  G01N29/02 ,  G01N1/28

CPC classification number: G01N35/00 ,  B01F11/0266 ,  C12Q1/04 ,  G01N1/28 ,  G01N1/286 ,  G01N1/38 ,  G01N33/487 ,  G01N35/00871 ,  G01N35/1009 ,  G01N2001/4094 ,  G01N2035/00524 ,  G01N2035/00554

Abstract: Devices, systems and methods including a sonicator for sample preparation are provided. A sonicator may be used to mix, resuspend, aerosolize, disperse, disintegrate, or de-gas a solution. A sonicator may be used to disrupt a cell, such as a pathogen cell in a sample. Sample preparation may include exposing pathogen-identifying material by sonication to detect, identify, or measure pathogens. A sonicator may transfer ultrasonic energy to the sample solution by contacting its tip to an exterior wall of a vessel containing the sample. Multipurpose devices including a sonicator also include further components for additional actions and assays. Devices, and systems comprising such devices, may communicate with a laboratory or other devices in a system for sample assay and analysis. Methods utilizing such devices and systems are provided. The improved sample preparation devices, systems and methods are useful for analyzing samples, e.g. for diagnosing patients suffering from infection by pathogens.

Abstract translation: 提供了包括用于样品制备的超声波发生器的装置，系统和方法。 超声波仪可用于混合，重悬，雾化，分散，分解或除气。 超声波仪器可用于破坏细胞，如样品中的病原体细胞。 样品制备可以包括通过超声处理暴露病原体识别材料以检测，鉴定或测量病原体。 超声波发生器可通过将其尖端接触到含有样品的容器的外壁将超声能量转移到样品溶液。 包括超声波发生器的多用途装置还包括用于附加动作和测定的其它组件。 设备和包括这种设备的系统可以与用于样品测定和分析的系统中的实验室或其他设备通信。 提供了利用这种装置和系统的方法。 改进的样品制备装置，系统和方法可用于分析样品，例如 用于诊断病原体感染的患者。