公开(公告)号：WO2016198748A1

公开(公告)日：2016-12-15

申请号：PCT/FI2016/050416

申请日：2016-06-10

Applicant: THERMO FISHER SCIENTIFIC OY

Inventor： RANNISTO, Joni ,  RAVELA, Suvi ,  FLOOK, Kelly J. ,  FINELL, Johan ,  VALMU, Leena ,  MARINOV, Borislav ,  SAARAINEN, Ville ,  POHL, Christopher A.

IPC: G01N30/08 ,  G01N30/60 ,  G01N1/40

CPC classification number: G01N30/08 ,  G01N30/6091 ,  G01N2001/4061 ,  G01N2030/009 ,  G01N2030/085

Abstract: Described are devices, systems, and methods directed to extraction columns. An embodiment of the extraction column includes a column body having a reservoir portion including an inlet and a reservoir, an extraction media portion, and a collar portion. The extraction media portion includes an elongated sleeve having a cavity having an extraction medium disposed therein. The extraction media portion has an inlet end and an outlet end. The collar portion extends axially with the elongated sleeve. The collar portion has a terminal end which is spaced apart from the outlet end of the elongated sleeve and which extends axially at least to a plane defined by the outlet end of the elongated sleeve. Also described is a sheath with a medium disposed therein, wherein the medium is separated from an inner surface of the sheath by a layer configured to substantially prevent a portion of sample from bypassing the medium.

Abstract translation: 描述的是针对提取列的设备，系统和方法。 提取塔的一个实施例包括具有包括入口和储存器的储存器部分，提取介质部分和套环部分的柱体。 提取介质部分包括具有其中设置有提取介质的空腔的细长套管。 提取介质部分具有入口端和出口端。 套环部分与细长套筒轴向延伸。 套环部分具有与细长套筒的出口端间隔开的端部，并且至少轴向延伸至由细长套管的出口端限定的平面。 还描述了其中布置有介质的护套，其中介质通过被配置为基本上防止一部分样品绕过介质的层与护套的内表面分离。

公开(公告)号：WO2018037089A1

公开(公告)日：2018-03-01

申请号：PCT/EP2017/071361

申请日：2017-08-24

Applicant: THERMO FISHER SCIENTIFIC OY

Inventor： KOSKINEN, Mikko ,  VALMU, Leena ,  RANNISTO, Joni ,  FLOOK, Kelly ,  GRIST, Roger ,  RANTAKARI, Anssi

IPC: G01N33/569 ,  G01N33/68

CPC classification number: G01N33/56944 ,  G01N33/569 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/56938 ,  G01N33/56961 ,  G01N33/6848

Abstract: The present invention includes a novel method and system for identification of microorganisms in samples that proteins and other biological material from non-microorganism sources (e.g., proteins of mammalian origin) that can interfere with identification of the microorganisms. The methods and systems described herein include use of a single-use chromatography medium to purify intact proteins prior to mass spectrometry analysis. The chromatography medium and the methods described herein can rapidly and efficiently remove of a substantial portion of interfering biological material (e.g., mammalian proteins) from a crude cell lysate while preserving high signal strength and removing enough of the interfering protein(s) to allow for identification of the microorganism(s) by mass spectrometry analysis.

Abstract translation: 本发明包括用于鉴定样品中微生物的新方法和系统，所述样品中来自非微生物来源（例如哺乳动物来源的蛋白质）的蛋白质和其他生物材料可干扰微生物的鉴定 。 本文所述的方法和系统包括在质谱分析之前使用单次使用的色谱介质来纯化完整蛋白质。 本文所述的色谱介质和方法可以从粗制细胞裂解物中快速且有效地除去大部分干扰性生物材料（例如，哺乳动物蛋白质），同时保持高信号强度并移除足够的干扰蛋白以允许 通过质谱分析鉴定微生物。

公开(公告)号：WO2022038542A1

公开(公告)日：2022-02-24

申请号：PCT/IB2021/057603

申请日：2021-08-18

Applicant: THERMO FISHER SCIENTIFIC OY

Inventor： RANNISTO, Joni

IPC: B01L3/00

Abstract: A filter sampling tool for filtering biological suspensions includes a body having a throughway extending through the body between a first end and an opposing second end, the body including a tubular sleeve portion having a first end and an opposing second end with a first passage extending therebetween, the first end of the tubular sleeve portion forming an opening that communicates with the first passage, the first passage forming at least a portion of the throughway of the body. An elongated stem has a first end and an opposing second end, the first end of the stem terminating at a tip, the stem being retained within the throughway of the body so that the first end of the stem passes through the opening at the first end of the tubular sleeve portion. A first fluid channel is formed between the stem and the body along a length of the stem disposed within the throughway so that fluid entering the body through the opening at the first end of the sleeve portion can travel along the first fluid channel and exit the body at the second end of the body.

公开(公告)号：WO2016132018A1

公开(公告)日：2016-08-25

申请号：PCT/FI2016/050104

申请日：2016-02-19

Applicant: THERMO FISHER SCIENTIFIC OY

Inventor： PELTOSAARI, Lauri ,  RANNISTO, Joni

IPC: B01L3/00

CPC classification number: B01L3/508 ,  B01L3/5082 ,  B01L3/50825 ,  B01L2200/025 ,  B01L2200/14 ,  B01L2300/021 ,  B01L2300/042 ,  B01L2300/043 ,  B01L2300/0858

Abstract: A sample vessel (1, 11) for transferring and analyzing a sample with analyzer instrument, which sample vessel comprises an elongated vessel body (2, 12) for containing the sample and a hinged cap (3, 13) connected in top end area of the vessel body, and the cross-section of the vessel body (2, 12) on a transversal plane in relation to the lengthwise center axis of the vessel body the inner surface of the vessel body is rotationally asymmetrical in relation to the lengthwise center axis, wherein the sample vessel (1, 11) comprises a surface (5, 15) extending upwards from the vessel body, which surface partially surrounds the edge of the cap (3, 13), when the cap is in closed position.

Abstract translation: 一种用于用分析仪器传送和分析样品的样品容器（1,11），所述样品容器包括用于容纳样品的细长容器体（2,12）和连接在所述样品容器的顶端区域中的铰接盖（3,13） 血管主体以及相对于血管主体的纵向中心轴的横向平面上的血管体（2,12）的横截面，血管主体的内表面相对于纵向中心轴线旋转地不对称 ，其中所述样品容器（1,11）包括当所述盖处于关闭位置时从所述容器主体向上延伸的表面（5,15），所述表面部分地围绕所述盖（3,13）的边缘。