公开(公告)号：WO2009082651A2

公开(公告)日：2009-07-02

申请号：PCT/US2008/087081

申请日：2008-12-17

Applicant: TRUSTEES OF DARTMOUTH COLLEGE ,  BZIK, David, J. ,  FOX, Barbara, A.

Inventor： BZIK, David, J. ,  FOX, Barbara, A.

IPC: C07H21/00 ,  C12N1/10 ,  A61K39/00 ,  C12Q1/02 ,  A61P43/00

CPC classification number: A61K39/012 ,  A61K2039/522 ,  C12N9/93 ,  G01N33/56905 ,  Y02A50/58

Abstract: Uracil auxotroph mutants of apicomplexans are provided which lack a functional carbamoyl phosphate synthase II (CPSII) enzyme. Also provided are T. gondii autoxtroph mutants which express exogenous antigens, and methods of protecting an animal against a T. gondii and non-T. gondii disease.

Abstract translation: 提供了缺乏功能性氨基甲酰磷酸合成酶II（CPSII）酶的复合物的尿嘧啶营养缺陷型突变体。 还提供了表达外源抗原的弓形虫自体突变体，以及保护动物抵抗弓形虫和非T型的方法。 弓形虫病

公开(公告)号：WO2013059411A1

公开(公告)日：2013-04-25

申请号：PCT/US2012/060746

申请日：2012-10-18

Applicant: TRUSTEES OF DARTMOUTH COLLEGE

Inventor： BZIK, David, J. ,  FOX, Barbara ,  FIERING, Steven, N. ,  CONEJO-GARCIA, Jose, R. ,  BAIRD, Jason ,  SANDERS, Kiah, L.

IPC: A61K39/12 ,  A61K39/00

CPC classification number: A61K39/015 ,  A61K39/002 ,  A61K2039/522 ,  A61K2039/523 ,  A61K2039/585 ,  C12N1/36 ,  C12N9/90 ,  C12N9/93

Abstract: The disclosure provides attenuated Toxoplasma gondii mutants for use as vaccines or treatment of cancer. More specifically, the attenuated mutants include a knockout mutation of one or more genes of the de novo pyrimidine synthesis pathway, or are generated by gamma-irradiation. Further disclosed are cancers that can be treated with the attenuated mutants.

Abstract translation: 本公开提供减毒弓形虫突变体用作疫苗或治疗癌症。 更具体地，减毒突变体包括一个或多个重要嘧啶合成途径的一个或多个基因的敲除突变，或者通过γ-照射产生。 进一步公开的是可用减毒突变体治疗的癌症。

公开(公告)号：WO1990001549A2

公开(公告)日：1990-02-22

申请号：PCT/US1989003422

申请日：1989-08-10

Applicant: TRUSTEES OF DARTMOUTH COLLEGE

Inventor： TRUSTEES OF DARTMOUTH COLLEGE ,  INSELBURG, Joseph, W. ,  BZIK, David, J.

IPC: C12N15/30

CPC classification number: C12N15/70 ,  A61K39/00 ,  C07K14/445 ,  Y02A50/412

Abstract: A Plasmodium falciparum gene encoding immunogenic SERA protein has been isolated by a) systematically screening a lambda gt11 recombinant DNA expression library with a murine monoclonal antibody directed against protein antigens of this pathogen, and b) systematically screening a lambda gt11 genomic cDNA and oligonucleotide probes directed against this pathogen. A 111 kDa protein has been shown to have immunogenic activity against parasite inhibitory antibodies. The gene encoding this protein, including the signal sequence and regulatory sequence in the adjacent 5' flanking sequence has been isolated and sequenced. Isolation and characterization of genes encoding major protein antigens of P. falciparum make it possible to develop reagents useful in the diagnosis, prevention and treatment of malaria. In addition, the signal sequences or regulatory sequences of this gene can be used to stimulate the production of other useful genetic products.

Abstract translation: 编码免疫原性SERA蛋白的恶性疟原虫基因已经通过以下方式分离：a）使用针对该病原体的蛋白质抗原的鼠单克隆抗体系统地筛选λgt11重组DNA表达文库，以及b）系统地筛选导向的λgt11基因组cDNA和寡核苷酸探针 针对这种病原体。 已显示111kDa蛋白具有针对寄生虫抑制性抗体的免疫原性活性。 已经分离并测序了编码该蛋白的基因，包括相邻5'侧翼序列中的信号序列和调控序列。 分离和表征编码恶性疟原虫的主要蛋白质抗原的基因使得有可能开发可用于诊断，预防和治疗疟疾的试剂。 此外，该基因的信号序列或调节序列可用于刺激其他有用的遗传产物的产生。

公开(公告)号：WO2014179038A1

公开(公告)日：2014-11-06

申请号：PCT/US2014/034127

申请日：2014-04-15

Applicant: TRUSTEES OF DARTMOUTH COLLEGE

Inventor： BZIK, David, J. ,  FOX, Barbara, A. ,  SANDERS, Kiah, L.

IPC: C12P1/00 ,  A61K39/012

CPC classification number: A61K39/0011 ,  A61K39/002 ,  A61K39/39 ,  A61K2039/522 ,  A61K2039/523 ,  A61K2039/57 ,  A61K2039/585 ,  C12N1/36 ,  C12N9/001 ,  C12N9/1018 ,  C12N9/1077 ,  C12N9/86 ,  C12N9/88 ,  C12N9/93

Abstract: Attenuated Toxoplasma gondii mutants and methods using the same as vaccines in the prevention or treatment pancreatic cancer are provided.

Abstract translation: 提供减毒的弓形体突变体和在预防或治疗胰腺癌中使用与疫苗相同的方法。

公开(公告)号：WO2009058712A1

公开(公告)日：2009-05-07

申请号：PCT/US2008/081274

申请日：2008-10-27

Applicant: TRUSTEES OF DARTMOUTH COLLEGE ,  BZIK, David, J. ,  FOX, Barbara, A.

Inventor： BZIK, David, J. ,  FOX, Barbara, A.

IPC: C12N1/10

CPC classification number: C12N9/90 ,  A61K39/015 ,  A61K2039/522 ,  A61K2039/523 ,  C12N9/93

Abstract: The present invention relates to a mutant Toxoplasma gondii which exhibits enhanced homologous recombination. The mutant of the present invention is a knockout in the KU80-dependent nonhomologous end-joining pathway which finds application in generating T. gondii gene knockouts and gene replacements for use in vaccine and drug development.

Abstract translation: 本发明涉及突变型弓形虫，其表现出增强的同源重组。 本发明的突变体是KU80依赖性非同源末端连接途径中的敲除，其可用于产生用于疫苗和药物开发的弓形虫基因敲除和基因替换。