公开(公告)号：WO2004020589A3

公开(公告)日：2005-02-10

申请号：PCT/US0326784

申请日：2003-08-28

Applicant: UNIV WASHINGTON ,  NEFF MICHAEL M

Inventor： NEFF MICHAEL M

IPC: C07K14/415 ,  C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  C12N5/04

CPC classification number: C07K14/415 ,  C12N15/8261 ,  Y02A40/146

Abstract: A transgenic plant transformed by a Dof transcription factor, OBP3, coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in an alteration in the size of the resulting plant as compared to a corresponding wild-type variety of plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Further provided are isolated OBP3's, isolated nucleic acid coding OBP3's, and vectors and host cells containing the latter.

Abstract translation: 通过Dof转录因子OBP3编码核酸转化的转基因植物，其中核酸序列在植物中的表达与相应的野生型多种植物相比导致所得植物大小的改变。 还提供了由转基因植物生产的农产品，包括种子。 还提供了分离的OBP3，分离的编码OBP3的核酸，以及含有后者的载体和宿主细胞。

公开(公告)号：WO0055302A3

公开(公告)日：2001-01-11

申请号：PCT/US0006915

申请日：2000-03-16

Applicant: SALK INST FOR BIOLOGICAL STUDI ,  NEFF MICHAEL M ,  CHORY JOANNE

Inventor： NEFF MICHAEL M ,  CHORY JOANNE

IPC: A01H5/00 ,  C07K16/40 ,  C12N5/10 ,  C12N9/02 ,  C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  A01H5/10 ,  C12N5/14

CPC classification number: C12N9/0071 ,  C12N9/0077 ,  C12N15/8243 ,  C12N15/8281 ,  C12N15/8286 ,  C12N15/8298 ,  Y02A40/162

Abstract: The present invention provides cytochrome P450s useful for producing genetically modified plants characterized as having the phenotypic trait of modulated brassinolide synthesis of signaling, for example, resulting in insect resistance, dwarfism and darker-green foliage compared with wild type plants. Such plants can be modified, for example, using "bas1", or functional homologues thereof, a polypeptide encoded by bas1 that modulates brassinolide synthesis and/or signaling in plants. The invention also provides methods for modulating ecdysteroid activity in a plant and for assaying brassinosteroid function in a plant. The latter method can be used to create a gain-of-function allelic series of plants characterized by increasing levels of overexpression of a cytochrome P450 to screen for brassinolide activity in plant species.

Abstract translation: 本发明提供可用于生产经遗传修饰的植物的细胞色素P450，其特征在于具有调节的油菜素内酯合成信号的表型性状，例如与野生型植物相比，导致昆虫抗性，矮化和深绿色叶。 这样的植物可以被修饰，例如使用“bas1”或其功能同系物，由bas1编码的多肽，其调节油菜素内酯合成和/或在植物中的信号转导。 本发明还提供了调节植物中蜕皮类固醇活性和测定植物中油菜素类固醇功能的方法。 后一种方法可用于产生功能增强的等位基因系列，其特征在于增加细胞色素P450的过表达水平以筛选植物物种中的油菜素内酯活性。