公开(公告)号：WO2023085955A1

公开(公告)日：2023-05-19

申请号：PCT/NZ2022/050140

申请日：2022-11-09

Applicant: VICTORIA LINK LIMITED ,  WAIKATOLINK LIMITED

Inventor： PATRICK, Wayne ,  OULAVALLICKAL, Tifany ,  ARCUS, Vickery Laurence ,  HICKS, Joanna

IPC: C12N15/10 ,  C12N15/70 ,  C12N9/00 ,  C12Q1/25 ,  C12Q1/6806

Abstract: The present disclosure encompasses RNA ligase enzymes, particularly thermostable RNA ligase enzymes, and including variant enzymes, along with the preparation and use thereof. Specifically encompassed are methods for preparing the RNA ligase enzymes, methods for ligation using these enzymes, as well as polynucleotides used for producing these enzymes, libraries comprising these polynucleotides, and microbial strains comprising these polynucleotides.