公开(公告)号：WO2017042814A1

公开(公告)日：2017-03-16

申请号：PCT/IL2016/051001

申请日：2016-09-08

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： REISNER, Yair ,  ZLOTNIKOV KLIONSKY, Yael ,  NATHANSOHN-LEVI, Bar ,  YARIMI, Liran ,  KAGAN, Sivan

IPC: C12N5/0784 ,  A61K39/00 ,  A61K35/15 ,  A61P37/00

CPC classification number: C12N5/064 ,  A01K67/0271 ,  A01K2267/0331 ,  A01K2267/0368 ,  A61K39/0008 ,  A61K2039/5154 ,  C12N2501/02 ,  C12N2501/04 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/22 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/26 ,  C12N2501/71

Abstract: A method of treating an inflammation in a subject in need thereof is disclosed. The method comprises administering to the subject a therapeutically effective amount of perforin+ immature DCs (Perf+ im DCs), thereby treating the inflammation in the subject. Methods of generating Perf+ im DCs, wherein the Perf+ im DCs are inhibited from maturing are also disclosed. Isolated population of cells, pharmaceutical compositions and articles of manufacture are also disclosed.

Abstract translation: 公开了治疗需要的受试者的炎症的方法。 该方法包括向受试者施用治疗有效量的穿孔素+未成熟DC（Perf + im DC），从而治疗受试者的炎症。 还公开了生成Perf + im DCs的方法，其中Perf + im DC被抑制成熟。 还公开了分离的细胞群，药物组合物和制品。

公开(公告)号：WO2018002924A1

公开(公告)日：2018-01-04

申请号：PCT/IL2017/050716

申请日：2017-06-27

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： REISNER, Yair ,  OR-GEVA, Noga ,  GIDRON BUDOVSKY, Rotem ,  BACHAR-LUSTIG, Esther ,  LASK, Assaf ,  KAGAN, Sivan

IPC: C12N5/0783 ,  A61K39/00 ,  A61K35/12 ,  A01K67/00

Abstract: A method of generating an isolated population of non graft versus host disease (GvHD) inducing cells comprising a central memory T-lymphocyte (Tcm) phenotype, the cells being tolerance inducing cells and/or endowed with anti-disease activity, and capable of homing to the lymph nodes following transplantation is disclosed. The method comprising: (a) providing a population of at least 70 % memory T cells; (b) contacting the population of memory T cells with an antigen or antigens so as to allow enrichment of antigen reactive cells; and (c) culturing the cells resulting from step (b) in the presence of cytokines so as to allow proliferation of cells comprising the Tcm phenotype. Cells generated by the method, pharmaceutical compositions and methods of treatment are also disclosed.

Abstract translation: 产生包含中央记忆T淋巴细胞（Tcm）表型的分离的非移植物抗宿主病（GvHD）细胞群的方法，所述细胞为耐受诱导细胞和/或赋予抗 - 疾病活动，并且能够在移植后归巢至淋巴结。 该方法包括：（a）提供至少70％记忆T细胞的群体; （b）使记忆T细胞群体与一种或多种抗原接触以便富集抗原反应性细胞; 和（c）在存在细胞因子的情况下培养步骤（b）产生的细胞，以允许包含Tcm表型的细胞增殖。 还公开了由该方法产生的细胞，药物组合物和治疗方法。

公开(公告)号：WO2017042816A1

公开(公告)日：2017-03-16

申请号：PCT/IL2016/051003

申请日：2016-09-08

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： REISNER, Yair ,  ZLOTNIKOV KLIONSKY, Yael ,  NATHANSOHN-LEVI, Bar ,  YARIMI, Liran ,  KAGAN, Sivan

IPC: C12N5/00 ,  A61K39/395 ,  A61P35/00 ,  A61K47/48

CPC classification number: C12N5/064 ,  A01K67/0271 ,  A01K2267/0331 ,  A01K2267/0368 ,  A61K39/0008 ,  A61K2039/5154 ,  C12N2501/02 ,  C12N2501/04 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/22 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/26 ,  C12N2501/71

Abstract: A method of treating a malignant disease in a subject in need thereof is disclosed. The method comprises subjecting cells of the subject to a therapeutically effective amount of an agent capable of ablating Perforin positive dendritic cells (Perf+ DCs) of the subject, thereby treating the malignant disease.

Abstract translation: 公开了在有需要的受试者中治疗恶性疾病的方法。 该方法包括使受试者的细胞经受治疗有效量的能够消融受试者的Perforin阳性树突细胞（Perf + DCs）的试剂，从而治疗恶性疾病。