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    METHOD OF MONOMERISATION OF RECOMBINANT ANTIBODY MOLECULES
    2.
    发明申请
    METHOD OF MONOMERISATION OF RECOMBINANT ANTIBODY MOLECULES 审中-公开
    重组抗体分子的单克隆方法

    公开(公告)号：WO2016170138A1

    公开(公告)日：2016-10-27

    申请号：PCT/EP2016/059051

    申请日：2016-04-22

    申请人： UCB BIOPHARMA SPRL

    发明人： HEYWOOD, Sam Philip ,  WILD, Gavin Barry ,  HANIF, Razwan ,  LE PAGE, Christopher John

    IPC分类号： C07K1/113 ,  C07K16/00

    CPC分类号： A61K39/39591 ,  C07C323/12 ,  C07C323/25 ,  C07K1/1133 ,  C07K1/1136 ,  C07K5/123 ,  C07K16/18 ,  C07K16/2878 ,  C07K2317/31

    摘要： The present invention provides method of increasing the percentage of monomer in a composition of recombinantly expressed antibody molecules characterised in that the antibody molecule comprises at least one Fv with specificity for an antigen of interest comprising one VH and one VL wherein said VH and VL are connected directly or indirectly via one or more linkers and are stabilised by a disulfide bond therebetween, said method comprises: a) a conversion step of treating the composition with a denaturant selected from urea and/or Guanidine hydrochloride; b) wherein step a) is performed in the presence of a reducing agent or after treatment with a reducing agent.

    摘要翻译： 本发明提供增加重组表达抗体分子组合物中单体百分比的方法，其特征在于抗体分子包含至少一种对目的抗原具有特异性的Fv，包括一个VH和一个VL，其中所述VH和VL连接 通过一个或多个接头直接或间接地通过其间的二硫键来稳定，所述方法包括：a）转化步骤，用选自脲和/或盐酸胍的变性剂处理组合物; b）其中步骤a）在还原剂存在下或用还原剂处理之后进行。

    D-APTIDE AND RETRO-INVERSO APTIDE WITH MAINTAINED TARGET AFFINITY AND IMPROVED STABILITY
    6.
    发明申请
    D-APTIDE AND RETRO-INVERSO APTIDE WITH MAINTAINED TARGET AFFINITY AND IMPROVED STABILITY 审中-公开
    具有维持目标程度的D-APTIDE和RETRO-INVERSO APTIDE和改进的稳定性

    公开(公告)号：WO2012138176A3

    公开(公告)日：2013-03-07

    申请号：PCT/KR2012002631

    申请日：2012-04-06

    申请人： KWANGJU INST SCI & TECH ,  JON SANG-YONG ,  KIM SUNG-HYUN

    发明人： JON SANG-YONG ,  KIM SUNG-HYUN

    IPC分类号： G01N33/68 ,  C07K2/00 ,  C40B40/10 ,  G01N33/53

    CPC分类号： C07K1/1136 ,  C07K14/00 ,  C07K14/001 ,  C12N15/115 ,  C12N2310/16 ,  G01N33/5308 ,  G01N33/573 ,  G01N2333/948

    摘要： The present invention is characterized by a D-aptamer-like peptide (D-Aptide) or retro-inverso Aptide which specifically binds to a target comprising: (a) a structure stabilizing region comprising parallel, antiparallel or parallel and antiparallel D-amino acid strands with interstrand noncovalent bonds; and (b) a target binding region I and a target binding region II comprising randomly selected n and m D-amino acids, respectively, and coupled to both ends of the structure stabilizing region. The D-Aptide or retro-inverso Aptide has the sequence of the same or opposite direction to L-Aptide, wherein the stability to proteases is improved while maintaining the affinity to a target compared with L-Aptide. The D-Aptide of the present invention has substantially the same target affinity and a remarkably improved stability compared with L-Aptide which is different from a general technical knowledge.

    摘要翻译： 本发明的特征在于与靶标特异性结合的D-适体样肽（D-Aptide）或逆转Aptide，其特征在于：（a）包含平行，反向平行或平行和反平行的D-氨基酸的结构稳定区 链间非共价键; 和（b）分别包含随机选择的n和m个D-氨基酸的靶结合区域I和靶结合区域II，并且结合到结构稳定区域的两端。 D-Aptide或逆转Aptide具有与L-Aptide相同或相反方向的序列，其中与L-Aptide相比，维持与靶标的亲和力提高了对蛋白酶的稳定性。 本发明的D-Aptide与一般技术知识不同，与L-Aptide相比，具有基本上相同的靶亲合力和显着提高的稳定性。

    METHODS FOR PROCESSING INCLUSION BODIES
    8.
    发明申请
    METHODS FOR PROCESSING INCLUSION BODIES 审中-公开
    处理包装体的方法

    公开(公告)号：WO2012054679A1

    公开(公告)日：2012-04-26

    申请号：PCT/US2011/057004

    申请日：2011-10-20

    申请人： MEDIMMUNE, LLC ,  O'CONNOR, Ellen, T.

    发明人： O'CONNOR, Ellen, T.

    IPC分类号： C07K1/00

    CPC分类号： C07K1/36 ,  C07K1/113 ,  C07K1/1133 ,  C07K1/1136 ,  C07K1/145 ,  C07K1/34

    摘要： The present application relates to methods for purifying recombinant proteins, including antibodies and antibody fragments. Suitably, the methods utilize depth filtration to clarify the desired proteins from a solubilized mixture, and provide refolding methods and refolding buffers to allow for refolding of the recombinant proteins into functional and active proteins. Exemplary antibody fragments include anti-CD22 antibody fragments that comprise V H and V L chains refolded into a functional and active fragment.

    摘要翻译： 本申请涉及用于纯化重组蛋白质的方法，包括抗体和抗体片段。 合适地，所述方法利用深度过滤来从溶解的混合物中澄清所需的蛋白质，并提供重折叠方法和重折叠缓冲液以允许将重组蛋白重折叠成功能性和活性蛋白质。 示例性抗体片段包括包含VH和VL链的抗-CD22抗体片段重折叠成功能和活性片段。

    METHODS FOR PREPARING MACROCYCLES AND MACROCYCLE STABILIZED PEPTIDES
    9.
    发明申请
    METHODS FOR PREPARING MACROCYCLES AND MACROCYCLE STABILIZED PEPTIDES 审中-公开
    制备大环化合物和大环化学稳定肽的方法

    公开(公告)号：WO2012051405A1

    公开(公告)日：2012-04-19

    申请号：PCT/US2011/056124

    申请日：2011-10-13

    申请人： BRISTOL-MYERS SQUIBB COMPANY ,  SAULNIER, Mark G. ,  VYAS, Dolatrai M. ,  LANGLEY, David R. ,  FRENNESSON, David B.

    发明人： SAULNIER, Mark G. ,  VYAS, Dolatrai M. ,  LANGLEY, David R. ,  FRENNESSON, David B.

    IPC分类号： C07K1/113 ,  C07K1/107 ,  C07D277/42

    CPC分类号： C07K7/64 ,  C07D277/42 ,  C07D417/04 ,  C07K1/1136 ,  C07K5/123 ,  C07K5/126

    摘要： The invention provides methods of preparing macrocycles including macrocycle stabilized peptides (MSPs). Macrocycles and MSPs are prepared according to nucleophilic capture of an iminoquinomethide type intermediate generated from a suitably substituted 2-amino-thiazol-5-yl carbinol. The preferred nucleophile may be selected from an electron rich aromatic moiety in the case of macrocycles and, in the case of MSPs, at least one amino acid comprises an electron rich aromatic moiety. In addition, the concept can be extended to other related 5-membered heterocyclic systems in place of the thiazole, such as imidazole or oxazole. The conditions for the generation of the corresponding iminoquinomethide type intermediates may be similar or different than the conditions used for the 2-amino-thiazol-5-yl carbinol.

    摘要翻译： 本发明提供了制备大环化合物包括大环稳定肽（MSP）的方法。 根据由适当取代的2-氨基 - 噻唑-5-基甲醇产生的亚氨基喹喔啉型中间体的亲核捕获制备大环和MSP。 在大环化合物的情况下，优选的亲核试剂可以选自富含电子的芳族部分，在MSP的情况下，至少一个氨基酸包含富电子的芳族部分。 此外，该概念可以扩展到其它相关的5元杂环体系代替噻唑，例如咪唑或恶唑。 产生相应的亚氨基喹喔啉型中间体的条件可以与用于2-氨基 - 噻唑-5-基甲醇的条件相似或不同。