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    GENE, ARS-R ANCHORAGE CASSETTE, ARS-R EXPRESSION- ANCHORAGE CASSETTE, RECOMBINANT PLASMID, BACTERIAL TRANSGENIC LINEAGE, USE OF SAID GENE, USE OF SAID LINEAGE IN ENVIRONMENTAL BIOREMEDIATION PROCESSES
    2.
    发明申请
    GENE, ARS-R ANCHORAGE CASSETTE, ARS-R EXPRESSION- ANCHORAGE CASSETTE, RECOMBINANT PLASMID, BACTERIAL TRANSGENIC LINEAGE, USE OF SAID GENE, USE OF SAID LINEAGE IN ENVIRONMENTAL BIOREMEDIATION PROCESSES 审中-公开
    基因，ARS-R锚定蛋白，ARS-R表达载体盒，重组质粒，细菌转基因系，使用基因，在环境生物过程中使用合适的序列

    公开(公告)号：WO2013173897A3

    公开(公告)日：2014-01-23

    申请号：PCT/BR2013000182

    申请日：2013-05-24

    Applicant: VALE SA ,  UNIV SAO PAULO ,  VICENTE ELISABETE JOSE ,  SCHENBERG ANA CLARA GUERRINI ,  DA SILVA PARADA CAROLINA ANGELICA ,  BIONDO RONALDO

    Inventor： VICENTE ELISABETE JOSE ,  SCHENBERG ANA CLARA GUERRINI ,  DA SILVA PARADA CAROLINA ANGELICA ,  BIONDO RONALDO

    IPC: C07K14/225

    CPC classification number: B09C1/10 ,  C02F2101/103 ,  C07K14/195 ,  C07K14/225 ,  C12N1/36

    Abstract: The present invention relates to the construction and insertion of a DNA plasmid vector of broad spectrum for Gram-negative bacteria, that carries a gene sequence which, when expressed, enables the anchorage of a chelating protein for arsenic ions on the Gram-negative bacteria cellular surface. For that end, the structural sequence of the regulatory arsR gene without stop codon (SEQ ID N° 1 ) was amplified by Polymerase Chain Reaction (PCR) using as a template the chromosome 1 of Cupriavidus metallidurans, CH34 lineage and inserted into the pGEM-T cloning vector, yielding the pGEMT-As plasmid (SEQ ID N° 2). The expression vector containing the sequence encoding the cassette for the expression and anchoring of heterologous proteins in Gram-negative bacteria, under the control of the pan promoter (SEQ. ID N° 3), was obtained upon digestion of the pCM-Hg plasmid with Xbal and Sa/I restriction enzymes. The arsR gene was released from the pGEMT-As plasmid by digestion with Xba\ and Sa/I restriction enzymes and then ligated to the linearized expression vector, called pCM (SEQ. ID N° 4), resulting in the construction of the pCM-As plasmid (SEQ ID N° 5). Additionally, the present invention provides recombinant strains of Gram-negative bacteria containing said recombinant plasmid, method of production, use of the recombinant plasmid to enhance bacterial arsenic resistance and capability to adsorb arsenic ions, as well as the use of the transgenic strains for the adsorption of arsenic ions in environmental bioremediation processes, with the possibility of recovering the metalloid as a byproduct.

    Abstract translation: 本发明涉及用于革兰氏阴性细菌的广谱谱DNA质粒载体的构建和插入，其携带的基因序列当其表达时能够在革兰氏阴性细菌细胞上锚定螯合蛋白质用于砷离子 表面。 为此，通过聚合酶链式反应（PCR），使用Cupriavidus metallidurans的染色体1（CH34谱系）作为模板扩增了无终止密码子（SEQ ID NO：1）的调节性arsR基因的结构序列，并插入到pGEM- T克隆载体，得到pGEMT-As质粒（SEQ ID NO：2）。 在pCM-Hg质粒消化后，获得了含有编码用于在泛启动子控制下的革兰氏阴性细菌中表达和锚定异源蛋白的序列的表达载体（SEQ ID NO：3） XbaI和Sa / I限制酶。 通过用XbaI和Sa / I限制酶消化从pGEMT-As质粒释放arsR基因，然后连接到称为pCM（SEQ ID NO：4）的线性表达载体上，导致构建pCM- 作为质粒（SEQ ID NO.5）。 此外，本发明提供含有所述重组质粒，生产方法，重组质粒的使用以增强细菌耐砷性和吸附砷离子的能力的革兰氏阴性菌的重组菌株，以及将转基因菌株用于 吸附砷离子在环境生物修复过程中，有可能回收准金属作为副产物。

    GENE, ARS-R ANCHORAGE CASSETTE, ARS-R EXPRESSION- ANCHORAGE CASSETTE, RECOMBINANT PLASMID, BACTERIAL TRANSGENIC LINEAGE, USE OF SAID GENE, USE OF SAID LINEAGE IN ENVIRONMENTAL BIOREMEDIATION PROCESSES
    5.
    发明申请
    GENE, ARS-R ANCHORAGE CASSETTE, ARS-R EXPRESSION- ANCHORAGE CASSETTE, RECOMBINANT PLASMID, BACTERIAL TRANSGENIC LINEAGE, USE OF SAID GENE, USE OF SAID LINEAGE IN ENVIRONMENTAL BIOREMEDIATION PROCESSES 审中-公开
    基因，ARS-R锚定蛋白，ARS-R表达载体盒，重组质粒，细菌转基因系，使用基因，在环境生物过程中使用合适的序列

    公开(公告)号：WO2013173897A2

    公开(公告)日：2013-11-28

    申请号：PCT/BR2013/000182

    申请日：2013-05-24

    Applicant: VALE S.A. ,  UNIVERSIDADE DE SÃO PAULO-USP ,  VICENTE, Elisabete José ,  SCHENBERG, Ana Clara Guerrini ,  DA SILVA PARADA, Carolina Angélica ,  BIONDO, Ronaldo

    Inventor： VICENTE, Elisabete José ,  SCHENBERG, Ana Clara Guerrini ,  DA SILVA PARADA, Carolina Angélica ,  BIONDO, Ronaldo

    IPC: C07K14/135

    CPC classification number: B09C1/10 ,  C02F2101/103 ,  C07K14/195 ,  C07K14/225 ,  C12N1/36

    Abstract: The present invention relates to the construction and insertion of a DNA plasmid vector of broad spectrum for Gram-negative bacteria, that carries a gene sequence which, when expressed, enables the anchorage of a chelating protein for arsenic ions on the Gram-negative bacteria cellular surface. For that end, the structural sequence of the regulatory arsR gene without stop codon (SEQ ID N° 1 ) was amplified by Polymerase Chain Reaction (PCR) using as a template the chromosome 1 of Cupriavidus metallidurans , CH34 lineage and inserted into the pGEM-T cloning vector, yielding the pGEMT-As plasmid (SEQ ID N° 2). The expression vector containing the sequence encoding the cassette for the expression and anchoring of heterologous proteins in Gram-negative bacteria, under the control of the pan promoter (SEQ. ID N° 3), was obtained upon digestion of the pCM-Hg plasmid with Xbal and Sa/I restriction enzymes. The arsR gene was released from the pGEMT-As plasmid by digestion with Xba\ and Sa/I restriction enzymes and then ligated to the linearized expression vector, called pCM (SEQ. ID N° 4), resulting in the construction of the pCM-As plasmid (SEQ ID N° 5). Additionally, the present invention provides recombinant strains of Gram-negative bacteria containing said recombinant plasmid, method of production, use of the recombinant plasmid to enhance bacterial arsenic resistance and capability to adsorb arsenic ions, as well as the use of the transgenic strains for the adsorption of arsenic ions in environmental bioremediation processes, with the possibility of recovering the metalloid as a byproduct.

    Abstract translation: 本发明涉及用于革兰氏阴性细菌的广谱谱DNA质粒载体的构建和插入，其携带的基因序列当其表达时能够在革兰氏阴性细菌细胞上锚定螯合蛋白质用于砷离子 表面。 为此，通过聚合酶链式反应（PCR），使用Cupriavidus metallidurans的染色体1（CH34谱系）作为模板扩增了无终止密码子（SEQ ID NO：1）的调节性arsR基因的结构序列，并插入到pGEM- T克隆载体，得到pGEMT-As质粒（SEQ ID NO：2）。 在pCM-Hg质粒消化后，获得了含有编码用于在泛启动子控制下的革兰氏阴性细菌中表达和锚定异源蛋白的序列的表达载体（SEQ ID NO：3） XbaI和Sa / I限制酶。 通过用XbaI和Sa / I限制酶消化从pGEMT-As质粒释放arsR基因，然后连接到称为pCM（SEQ ID NO：4）的线性表达载体上，导致构建pCM- 作为质粒（SEQ ID NO.5）。 此外，本发明提供含有所述重组质粒，生产方法，重组质粒的使用以增强细菌耐砷性和吸附砷离子的能力的革兰氏阴性菌的重组菌株，以及将转基因菌株用于 吸附砷离子在环境生物修复过程中，有可能回收准金属作为副产物。

    CONTROL OF LEAF SCALD DISEASE
    8.
    发明申请
    CONTROL OF LEAF SCALD DISEASE 审中-公开
    叶鳞病的控制

    公开(公告)号：WO1997009417A1

    公开(公告)日：1997-03-13

    申请号：PCT/AU1996000554

    申请日：1996-09-06

    Applicant: THE UNIVERSITY OF QUEENSLAND ,  BIRCH, Robert ,  ZHANG, Lianhui

    Inventor： THE UNIVERSITY OF QUEENSLAND

    IPC: C12N01/20

    CPC classification number: C12R1/18 ,  A01N63/00 ,  C07K14/225 ,  C12N9/00 ,  C12N9/14 ,  C12N15/74 ,  C12N15/8281

    Abstract: A method of substantially reducing or inhibiting the development of leaf scald disease in a plant or stalk thereof, said method comprising the step of administering an albicidin detoxification enzyme to the plant or stalk thereof. There is also provided a method of generating a transgenic plant substantially resistant to albicidin and leaf scald disease including the steps of introducing and expressing a nucleotide sequence encoding albicidin detoxification enzyme into a plant, plant part or plant cell, and growing the plant, plant part or plant cell to generate the transgenic plant. There is further provided a method of substantially reducing or inhibiting the development of leaf scald disease in a plant or stalk thereof, said method comprising the step of administering to the plant or stalk thereof a bacterium which extracellularly produces albicidin detoxification enzyme. There is further provided an isolated albicidin detoxification enzyme capable of irreversibly inactivating albicidin as well as an isolated nucleotide sequence encoding an albicidin detoxification enzyme.

    Abstract translation: 一种显着减少或抑制植物或其茎中叶片烫伤疾病发展的方法，所述方法包括向植物或其茎施用albicidin解毒酶的步骤。 还提供了一种产生对albicidin和叶片烫伤病基本上抗性的转基因植物的方法，包括以下步骤：将编码albicidin解毒酶的核苷酸序列引入植物，植物部分或植物细胞，并使植物，植物部分 或植物细胞以产生转基因植物。 进一步提供了一种在植物或其茎中显着降低或抑制叶片烫伤疾病发展的方法，所述方法包括向植物或茎施用细胞外产生albicidin解毒酶的细菌的步骤。 还提供了能够不可逆地灭活albicidin的分离的albicidin解毒酶以及编码albicidin解毒酶的分离的核苷酸序列。

    RECOMBINANT MICROBIAL FERTILIZER AND METHODS FOR ITS PRODUCTION
    9.
    发明申请
    RECOMBINANT MICROBIAL FERTILIZER AND METHODS FOR ITS PRODUCTION 审中-公开
    重组微生物肥料及其生产方法

    公开(公告)号：WO1995004814A1

    公开(公告)日：1995-02-16

    申请号：PCT/US1994008846

    申请日：1994-08-05

    Applicant: INTERNATIONAL TLB RESEARCH INSTITUTE, INC.

    Inventor： INTERNATIONAL TLB RESEARCH INSTITUTE, INC. ,  ZHANG, Ling, Yu

    IPC: C12N01/00

    CPC classification number: C05F11/08 ,  C07K14/225 ,  C07K14/32 ,  C07K14/395 ,  C12N15/76

    Abstract: A microbial fertilizer that constitutes a symbiotic association of several recombinant microbial species is described. The fertilizer contains four streptomyces strains (5 and 8) and two yeast strains (H4 (1) and H7 (3)). The streptomyces strains include a nitrogen fixing strain, a phosphorus decomposer, a potassium decomposer and a coal waste decomposer. The yeast strains produce growth factors and energy required by the streptomyces.

    Abstract translation: 描述了构成几种重组微生物物种的共生关联的微生物肥料。 肥料含有四种链霉菌菌株（5和8）和两种酵母菌株（H4（1）和H7（3））。 链霉菌菌株包括固氮菌株，磷分解器，钾分解器和煤废物分解器。 酵母菌株产生链霉菌所需的生长因子和能量。

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