公开(公告)号：WO2017070029A1

公开(公告)日：2017-04-27

申请号：PCT/US2016/057272

申请日：2016-10-17

Applicant: PIONEER HI-BRED INTERNATIONAL, INC.

Inventor： CIGAN, Andrew Mark ,  SVITASHEV, Sergei

IPC: C12N9/22 ,  C12N15/01 ,  C12N15/82 ,  C12N15/90

CPC classification number: C12N15/8206 ,  C07K2319/09 ,  C12N9/22 ,  C12N15/8207 ,  C12N15/8241 ,  C12N15/902

Abstract: Compositions and methods are provided for modifying a nucleotide sequence in the genome of a plant cell, without the use of a selectable marker. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to make a double strand break in a target site located in a nucleotide sequence and plant cells are obtained without the use of a selectable marker, and to provide an effective system for modifying target sites within the genome of a plant, plant cell or seed. Compositions and methods are also provided for producing a plant cell, callus tissue or plant having a modified nucleotide sequence in its genome, without the use of a selectable marker.

Abstract translation: 提供组合物和方法用于修饰植物细胞基因组中的核苷酸序列，而不使用选择标记。 所述方法和组合物使用引导多核苷酸/ Cas核酸内切酶系统在位于核苷酸序列中的靶位点中产生双链断裂，并且在不使用选择标记的情况下获得植物细胞，并提供用于修饰靶位点的有效系统 在植物，植物细胞或种子的基因组内。 还提供了组合物和方法，用于在其基因组中产生具有修饰的核苷酸序列的植物细胞，愈伤组织或植物，而不使用选择标记。

公开(公告)号：WO2016149352A1

公开(公告)日：2016-09-22

申请号：PCT/US2016/022621

申请日：2016-03-16

Applicant: PIONEER HI-BRED INTERNATIONAL INC

Inventor： ELSING, Michael T. ,  GORDON-KAMM, William ,  PERUGINI, Leandro Daniel ,  YE, Huaxun

IPC: A01H1/04 ,  C12N15/82

CPC classification number: C12N15/8241 ,  A01H1/04 ,  C12N15/8212 ,  C12N15/8213 ,  C12N15/825 ,  C12N15/8289

Abstract: Compositions and methods are provided for the use of pollen-inhibitor genes and/or color marker genes in accelerated trait introgression. Compositions and methods are also provided for introducing a pollen-inhibitor gene and/or a color marker gene in close proximity to a trait locus of interest. Breeding methods and methods for selecting plants comprising a trait locus of interest in close proximity to at least one pollen-inhibitor gene and/ or color marker gene are also disclosed. The methods and compositions employ at least one pollen-inhibitor gene and /or color marker gene to provide an effective system for accelerated trait introgression in the genome of a plant.

Abstract translation: 提供组合物和方法用于在加速性状渗入中使用花粉抑制基因和/或颜色标记基因。 还提供组合物和方法用于在感兴趣的性状位点附近引入花粉抑制基因和/或颜色标记基因。 还公开了用于选择包含非常接近至少一个花粉抑制基因和/或颜色标记基因的感兴趣性状基因座的植物的育种方法和方法。 方法和组合物使用至少一种花粉抑制基因和/或颜色标记基因来提供植物基因组中加速性状渗入的有效系统。

公开(公告)号：WO2016119703A1

公开(公告)日：2016-08-04

申请号：PCT/CN2016/072352

申请日：2016-01-27

Applicant: 中国科学院遗传与发育生物学研究所

Inventor： 高彩霞 ,  张毅 ,  吴忠义 ,  张康

IPC: C12N15/89 ,  C12N15/84

CPC classification number: C12N15/8216 ,  C12N9/22 ,  C12N15/8201 ,  C12N15/8213 ,  C12N15/8241 ,  C12N15/89 ,  C12N2310/20

Abstract: 提供一种通过基因瞬时表达对整株植物中目的基因靶标片段进行定点改造的方法，包括以下步骤：以整株植物为瞬时表达对象，在植物中瞬时表达特异于目的基因靶标片段的核酸酶，在核酸酶的作用下，靶标片段被剪切，通过植物的自身DNA修复，实现对靶标片段的定点改造。还提供了利用该方法培育非转基因突变植株的方法和所获得的非转基因突变植株。

公开(公告)号：WO2015189376A1

公开(公告)日：2015-12-17

申请号：PCT/EP2015/063144

申请日：2015-06-12

Applicant: SESVANDERHAVE N.V.

Inventor： WEYENS, Guy ,  LEFÈBVRE, Marc ,  THAMMANNAGOWDA, Shivegowda ,  LOMMEL, Murielle

IPC: C07K14/415 ,  C12N15/82

CPC classification number: C12N15/821 ,  A01H1/02 ,  A01H1/04 ,  C12N9/88 ,  C12N15/52 ,  C12N15/8209 ,  C12N15/8241 ,  C12N15/8279 ,  C12N15/8281 ,  C12N15/8282 ,  C12N15/8285 ,  C12N15/8286

Abstract: The present invention is related to a method for the transformation of sugar beet protoplasts comprising the steps of: – obtaining protoplasts from stomatal guard cells isolated from a sugar beet plant, - transforming the protoplasts with a nucleic acid construct comprising a nucleotide sequence of interest and a selection marker sequence, - applying to an in vitro culture of said protoplasts, one or more ALS inhibitors at a concentration that is lethal to the in vitro culture of the protoplasts and - regenerating sugar beet plants from the surviving protoplasts having integrated the nucleic acid construct comprising the sequence of interest and the selection marker sequence, wherein the selectionmarker sequence is the mutated BvALS113 sequence carrying in its sequence a mutation at amino acid 113 position from Alanine to Tyrosine.

Abstract translation: 本发明涉及一种用于转化甜菜原生质体的方法，包括以下步骤： - 从分离自甜菜植物的气孔保护细胞获得原生质体， - 用包含感兴趣的核苷酸序列的核酸构建体转化原生质体，以及 选择标记序列， - 以对原生质体的体外培养致死的浓度应用所述原生质体的一种或多种ALS抑制剂的体外培养，以及从具有整合核酸的存活的原生质体再生甜菜植物 构建体，其包含感兴趣的序列和选择标记序列，其中选择标记序列是突变的BvALS113序列，其在氨基酸113位从氨基酸到酪氨酸的突变。

公开(公告)号：WO2015132712A1

公开(公告)日：2015-09-11

申请号：PCT/IB2015/051520

申请日：2015-03-02

Applicant: BASF PLANT SCIENCE COMPANY GMBH ,  BASF (CHINA) COMPANY LIMITED

Inventor： SANZ MOLINERO, Ana Isabel

IPC: C12N15/82 ,  C07K14/415 ,  C12N15/29 ,  A01H5/00 ,  C12N5/04 ,  C12N15/63

CPC classification number: C12N15/8261 ,  C07K14/415 ,  C12N15/8241 ,  C12N15/827 ,  C12N15/8273 ,  Y02A40/146

Abstract: Provided is a method for enhancing one or more yield-related traits in a plant by modulating expression in the plant of a nucleic acid encoding an ANAC055(Arabidopsis No Apical Meristem, Arabidopsis Transcription Factor, Cup-shaped Cotyledon) polypeptide. Also provided are plants having modulated expression of a nucleic acid encoding an ANAC055 polypeptide, which plants have one or more enhanced yield-related traits compared with control plants. Further provided are hitherto unknown ANAC055-encoding nucleic acids, and constructs comprising the same, useful in performing the method.

Abstract translation: 提供了一种通过调节植物中编码ANAC055（拟南芥无顶生分生组织，拟南芥转录因子，杯形子叶）多肽的核酸表达来增强植物中一种或多种产量相关性状的方法。 还提供了具有调节表达编码ANAC055多肽的核酸的植物，该植物与对照植物相比具有一种或多种增强的产量相关性状。 进一步提供了迄今为止未知的ANAC055编码核酸，以及包含该核酸的构建体，其用于实施该方法。

公开(公告)号：WO2015054111A1

公开(公告)日：2015-04-16

申请号：PCT/US2014/059261

申请日：2014-10-06

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： BELL, Erin ,  QI, Qungang ,  RAJANI, Monnanda

IPC: A01H1/00

CPC classification number: C12N15/8261 ,  C07K14/415 ,  C12N15/8241 ,  C12N15/8273 ,  Y02A40/146

Abstract: This disclosure provides transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.

Abstract translation: 本公开提供具有增强性状的转基因植物，例如增加的产量，增加的氮利用效率和增强的耐旱性; 这些转基因植物的繁殖体，后代和田间作物; 以及制备和使用这些转基因植物的方法。 本公开还提供了从这样的转基因植物产生种子的方法，种植这种种子并选择具有增强性状的后代植物。 还公开了具有改变的表型的转基因植物，其可用于筛选和选择所需增强性状的转基因事件。

公开(公告)号：WO2015054106A1

公开(公告)日：2015-04-16

申请号：PCT/US2014/059253

申请日：2014-10-06

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： ABAD, Mark, Scott ,  ALLEN, Edwards, M. ,  ANIL, Veena, S. ,  AUGUSTINE, Alice, Clara ,  BANKS, Isaac, R. ,  BHAT, Prasanna, R. ,  CHITTOOR-VIJAYANATH, Jaishree, M. ,  CHOMET, Paul, S. ,  DENG, Molian ,  DUFF, Stephen ,  GOLDMAN, Barry, S. ,  IVASHUTA, Sergey ,  KARUNANANDAA, Balasulojini ,  LUTFIYYA, Linda ,  MANJUNATH, Sivalinganna ,  NEELAM, Anil ,  RAJANI, Monnanda, S. ,  RAMAMOHAN, G. ,  SHARMA, Chitresh ,  SHOBHA, Char ,  VENKATESH, Tyamagondlu V. ,  VIDYA, Kammaradi R. ,  ZHAO, Jianmin

IPC: A01H4/00

CPC classification number: C12N15/8241 ,  A01H1/02 ,  C12N15/113 ,  C12N15/8261 ,  C12N15/8271 ,  C12N2310/141

Abstract: This disclosure provides transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing hybrid seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.

Abstract translation: 本公开提供具有增强性状的转基因植物，例如增加的产量，增加的氮利用效率和增强的耐旱性; 这些转基因植物的繁殖体，后代和田间作物; 以及制备和使用这些转基因植物的方法。 本公开还提供了从这种转基因植物产生杂种种子的方法，种植这种种子并选择具有增强特征的后代植物。 还公开了具有改变的表型的转基因植物，其可用于筛选和选择所需增强性状的转基因事件。

公开(公告)号：WO2015029031A1

公开(公告)日：2015-03-05

申请号：PCT/IL2014/050769

申请日：2014-08-26

Applicant: EVOGENE LTD.

Inventor： GIL, Lidor ,  RIMON KNOPF, Ronit ,  MATARASSO, Noa ,  PANIK, David ,  PORATY-GAVRA, Limor ,  KATZ, Ehud ,  KARCHI, Hagai

IPC: A01H5/00 ,  C12N15/82 ,  C12N15/29 ,  C07K14/415

CPC classification number: C12N15/8261 ,  C07K14/415 ,  C12N15/8241 ,  C12N15/8243 ,  C12N15/8247 ,  C12N15/8271 ,  C12N15/8273 ,  Y02A40/146

Abstract: Provided are isolated polypeptides which are at least 80% homologous to SEQ ID NOs: 710-1153 and 9276-15726, isolated polynucleotides which are at least 80% identical to SEQ ID NOs: 1-709 and 1157-9275, nucleic acid constructs comprising same, transgenic cells expressing same, transgenic plants expressing same and method of using same for increasing yield, abiotic stress tolerance, growth rate, biomass, vigor, oil content, photosynthetic capacity, seed yield, fiber yield, fiber quality, fiber length, and/or nitrogen use efficiency of a plant.

Abstract translation: 提供的是与SEQ ID NO：710-1153和9276-15726至少80％同源的分离的多肽，与SEQ ID NO：1-709和1157-9275至少80％相同的分离的多核苷酸，包含 表达相同的转基因细胞，表达相同的转基因植物和使用该植物提高产量，非生物胁迫耐受性，生长速率，生物量，活力，油含量，光合能力，种子产量，纤维产量，纤维质量，纤维长度的方法和 /或植物的氮利用效率。

公开(公告)号：WO2015026887A1

公开(公告)日：2015-02-26

申请号：PCT/US2014/051782

申请日：2014-08-20

Applicant: E. I. DU PONT DE NEMOURS AND COMPANY ,  PIONEER HI-BRED INTERNATIONAL, INC.

Inventor： CIGAN, Andrew, Mark ,  FALCO, Saverio, Carl ,  LI, Zhongsen ,  LIU, Zhan-Bin ,  YOUNG, Joshua, K.

IPC: C12N15/82 ,  C12N15/8213 ,  C07K14/415

CPC classification number: C12N15/8216 ,  C12N15/113 ,  C12N15/81 ,  C12N15/8213 ,  C12N15/8241 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8279 ,  C12N15/8286 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/315 ,  C12N2310/3231 ,  C12N2310/3341 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12N2310/321 ,  C12N2310/3521 ,  C12N2310/322 ,  C12N2310/3533

Abstract: The disclosure relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.

Abstract translation: 本公开涉及来自大豆，特别是大豆U6聚合酶III基因的启动子及其片段的基因表达调控序列及其在促进植物中一种或多种异源核酸片段的表达的用途。 本发明还公开了组合物，多核苷酸构建体，转化的宿主细胞，突变植物，转基因植物和含有重组构建体的启动子的种子，以及制备和使用该重组构建体的方法。

公开(公告)号：WO2015017637A1

公开(公告)日：2015-02-05

申请号：PCT/US2014/049093

申请日：2014-07-31

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： STASKAWICZ, Brian, J. ,  DAHLBECK, Douglas

IPC: C12N15/82

CPC classification number: C12N15/8281 ,  A01G22/00 ,  A01H1/02 ,  A01H4/008 ,  A01H5/08 ,  C07K14/415 ,  C12N15/8241 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/158

Abstract: The invention provides seed and plants of tomato comprising transgenic event Bs2-X5 and progeny thereof comprising this transgenic event and uses of such seeds and plants in the production of tomato fruit. The invention thus relates to non-hybrid and hybrid plants, seeds, and tissue cultures of tomato comprising transgenic event Bs2-X5, and to methods for producing a tomato plant produced by crossing such plants with themselves or with another tomato plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

Abstract translation: 本发明提供番茄的种子和植物，其包含转基因事件Bs2-X5及其后代，其包含该转基因事件以及这种种子和植物在番茄果实生产中的用途。 因此，本发明涉及包含转基因事件Bs2-X5的番茄的非杂交和杂交植物，种子和组织培养物，以及用于产生通过将这些植物与其自身或其他番茄植物杂交（例如 另一种基因型植物。 本发明还涉及通过这种交叉产生的种子和植物。 本发明还涉及这种植物的部分，包括这种植物的水果和配子。