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    METHODS AND VECTORS TO PRODUCE VECTOR FREE INDUCED PLURIPOTENT STEM CELLS
    2.
    发明申请
    METHODS AND VECTORS TO PRODUCE VECTOR FREE INDUCED PLURIPOTENT STEM CELLS 审中-公开
    方法和向量生产矢量免费诱导多功能干细胞

    公开(公告)号：WO2017123789A1

    公开(公告)日：2017-07-20

    申请号：PCT/US2017/013229

    申请日：2017-01-12

    Applicant: LONZA WALKERSVILLE, INC.

    Inventor： ABRAHAM, Eytan ,  PAYNE, Thomas ,  YOUNG, Robert, J. ,  FRIEDRICH BEN NUN, Inbar

    IPC: A61K35/545 ,  C12N5/00 ,  C12N5/04 ,  C12N5/06 ,  C12N15/63

    CPC classification number: A61K35/545 ,  C12N5/0696 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2510/00

    Abstract: The invention relates generally to methods of generating induced pluripotent stem cells (iPSCs) that do not contain the reprogramming vector. In some embodiments,, the invention relates to inducing pluripotency in somatic cells by introducing an episomal vector(s) comprising at least one expression cassette containing reprogramming factors and/or synthetic transcription factors and a suicide gene. In some embodiments, the invention relates to inducing pluripotency in somatic cells by introducing episomal vector(s) comprising expression cassettes containing reprogramming factors and/or synthetic transcription factors and a transcriptionally regulated EBNA-1 gene. In some embodiments, the invention relates to inducing pluripotency in somatic cells by introducing episomal vector(s) comprising expression cassettes containing reprogramming factors and/or synthetic transcription factors and both a suicide gene and a transcriptionally regulated EBNA-1 gene.

    Abstract translation: 本发明一般涉及产生不含重编程载体的诱导性多能干细胞（iPSC）的方法。 在一些实施方案中，本发明涉及通过引入包含至少一个含重编程因子和/或合成转录因子和自杀基因的表达盒的游离型载体在体细胞中诱导多能性。 在一些实施方案中，本发明涉及通过引入包含含有重编程因子和/或合成转录因子的表达盒和转录调节的EBNA-1基因的游离型载体来诱导体细胞中的多能性。 在一些实施方案中，本发明涉及通过引入包含含有重编程因子和/或合成转录因子以及自杀基因和转录调节的EBNA-1基因的表达盒的游离型载体在体细胞中诱导多能性。

    FEEDER-FREE DERIVATION OF HUMAN-INDUCED PLURIPOTENT STEM CELLS WITH SYNTHETIC MESSENGER RNA
    5.
    发明申请
    FEEDER-FREE DERIVATION OF HUMAN-INDUCED PLURIPOTENT STEM CELLS WITH SYNTHETIC MESSENGER RNA 审中-公开
    免疫衍生的人类诱导的具有合成信使RNA的细胞干细胞

    公开(公告)号：WO2013173248A3

    公开(公告)日：2014-12-18

    申请号：PCT/US2013040814

    申请日：2013-05-13

    Applicant: ALLELE BIOTECHNOLOGY AND PHARMACEUTICALS INC

    Inventor： WANG JIWU ,  WARREN LUIGI ,  NI YUHUI

    IPC: C12N5/10

    CPC classification number: C12N5/0696 ,  C12N15/111 ,  C12N2310/14 ,  C12N2320/30 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/605 ,  C12N2501/608 ,  C12N2506/1307 ,  C12N2510/00

    Abstract: The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.

    Abstract translation: 本公开一般涉及通过动力学控制的过程使用工程化重编程因子来产生诱导多能干细胞（iPSC）的新方法和组合物。 具体地，本公开涉及建立重编程因子的组合，包括常规重编程因子与反式激活结构域之间的融合，优化用于重新编程各种类型的细胞。 更具体地，本文公开的示例性方法可用于从各种哺乳动物细胞类型（包括人成纤维细胞）产生诱导多能干细胞。 还公开了使用合成信使RNA的人诱导多能干细胞的无饲养物衍生的示例性方法。

    METHODS AND COMPOSITIONS FOR GENERATION OF INDUCED PLURIPOTENT STEM CELLS BY RNAA
    7.
    发明申请
    METHODS AND COMPOSITIONS FOR GENERATION OF INDUCED PLURIPOTENT STEM CELLS BY RNAA 审中-公开
    通过RNAA产生诱导的大脑干细胞的方法和组合物

    公开(公告)号：WO2012027486A3

    公开(公告)日：2014-03-27

    申请号：PCT/US2011048985

    申请日：2011-08-24

    Applicant: UNIV CALIFORNIA ,  LI LONG-CHENG

    Inventor： LI LONG-CHENG

    IPC: C12N5/02 ,  C07H21/04 ,  C12N5/071 ,  C12N15/00

    CPC classification number: C12N5/0696 ,  C12N15/111 ,  C12N2310/13 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606

    Abstract: The present disclosure provides methods for inducing somatic cells to form induced pluripotent stem (iPS) cells. The method includes introducing a small activating RNA (saRNA) molecule into the somatic cell, where the saRNA molecule increases transcription of a transcription factor that induces the formation of induced pluripotent stem cells. The present disclosure also provides compositions and kits comprising a saRNA molecule that increases transcription of a transcription factor that induces the formation of induced pluripotent stem cells. The present disclosure provides iPS cells comprising at least one exogenous saRNA molecule, where the saRNA molecule increases transcription of a transcription factor that induces the formation of induced pluripotent stem cells.

    Abstract translation: 本公开提供诱导体细胞以形成诱导多能干细胞（iPS）细胞的方法。 该方法包括将小活化RNA（saRNA）分子引入体细胞，其中saRNA分子增加诱导诱导的多能干细胞形成的转录因子的转录。 本公开还提供了包含增加诱导诱导的多能干细胞形成的转录因子的转录的saRNA分子的组合物和试剂盒。 本公开提供包含至少一种外源性saRNA分子的iPS细胞，其中所述saRNA分子增加诱导诱导多能干细胞形成的转录因子的转录。

    A PROTEIN-INDUCED PLURIPOTENT CELL TECHNOLOGY USES THEREOF
    9.
    发明申请
    A PROTEIN-INDUCED PLURIPOTENT CELL TECHNOLOGY USES THEREOF 审中-公开
    蛋白质诱导的细胞周期细胞技术

    公开(公告)号：WO2012151234A2

    公开(公告)日：2012-11-08

    申请号：PCT/US2012/036051

    申请日：2012-05-02

    Applicant: WAYNE STATE UNIVERSITY ,  WANG, Jianjun ,  LI, Qianqian

    Inventor： WANG, Jianjun ,  LI, Qianqian

    IPC: C12N5/074

    CPC classification number: C12N5/0696 ,  C12N2501/115 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2506/1307

    Abstract: A method of generating protein-induced pluripotent stem cells by delivering bacterial!y expressed reprogramming proteins into nuclei of starting somatic cells using the QQ-protein transduction technique, repeating several cell reprogramming cycles for creating reprogrammed protein-induced pluripotent stem cells, moving the reprogrammed cells into a feeder-free medium for expansion, and expanding and passaging the reprogrammed cells in a whole dish for generating homogeneous piPS cells. Also provided are the piPCS cells formed using this method and uses thereof.

    Abstract translation: 通过使用QQ蛋白转导技术将细菌表达的重编程蛋白递送到起始体细胞的核中产生蛋白质诱导的多能干细胞的方法，重复几个细胞重编程循环，用于产生重编程的蛋白质诱导的多能干细胞，移动重编程 细胞进入无饲养层的培养基中进行扩增，并在整个培养皿中扩增和传代重编程细胞以产生均质的piPS细胞。 还提供了使用该方法形成的piPCS细胞及其用途。

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