公开(公告)号：WO2018218485A1

公开(公告)日：2018-12-06

申请号：PCT/CN2017/086556

申请日：2017-05-31

Applicant: BEIJING PERCANS ONCOLOGY CO. LTD.

Inventor： ZHANG, Yihong ,  YANG, Zhen

IPC: C12N5/00 ,  C12N5/02

CPC classification number: C12N5/0693 ,  C12N5/0031 ,  C12N5/0043 ,  C12N2500/38 ,  C12N2501/155 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2503/02

Abstract: Provided are a cell culture medium and a method of culturing or expanding human epithelial tumor cells from cancer-tissue originated spheroids (CTOSs). Also provided is a method of testing responsiveness of a human patient to one or more therapeutic agents.

公开(公告)号：WO2017160978A1

公开(公告)日：2017-09-21

申请号：PCT/US2017/022501

申请日：2017-03-15

Applicant: BOSTON BIOMEDICAL, INC.

Inventor： LI, Chiang, Jia ,  ROGOFF, Harry, A. ,  SIMON, Karen

IPC: C12N5/09 ,  C12N5/095 ,  G01N33/50

CPC classification number: C12N5/0062 ,  C12N5/0068 ,  C12N5/0693 ,  C12N5/0695 ,  C12N2501/999 ,  C12N2503/02 ,  C12N2513/00 ,  C12N2533/74 ,  C12N2537/10 ,  G01N33/5011 ,  G01N33/5073 ,  G01N2500/00

Abstract: The present disclosure provides an alginate-based 3D cell culture as an in vitro system for enriching and maintaining the sternness properties of a cancer cell line and a reliable in vitro system for the development and evaluation of CSC-targeting agents.

Abstract translation: 本公开内容提供了基于藻酸盐的3D细胞培养物作为用于富集和维持癌细胞系的严格性质的体外系统，并且提供了可靠的<！ - SIPO

公开(公告)号：WO2017156341A1

公开(公告)日：2017-09-14

申请号：PCT/US2017/021683

申请日：2017-03-09

Applicant: BEIJING PERCANS ONCOLOGY CO. LTD.

Inventor： CHEN, Yiyou ,  YANG, Zhen ,  ZHANG, Yihong

IPC: C12N5/09 ,  C12N5/02

CPC classification number: C12N5/0693 ,  C12N5/0062 ,  C12N5/04 ,  C12N2513/00 ,  G01N33/5011 ,  G01N33/574 ,  G01N2510/00 ,  G01N2800/52

Abstract: Provided are suspension-based cell culture systems and media for the timely and efficient proliferation of human tumor cell clusters from a patient, and related methods of evaluating the potential responsiveness of the tumor cells and the patient to one or more therapeutic agents.

Abstract translation: 提供了基于悬浮液的细胞培养系统和用于从患者中及时有效增殖人类肿瘤细胞簇的培养基，以及评估肿瘤细胞和患者对一种肿瘤细胞的潜在反应性的相关方法 或更多的治疗剂。

公开(公告)号：WO2017093531A1

公开(公告)日：2017-06-08

申请号：PCT/EP2016/079664

申请日：2016-12-02

Applicant: UNIVERSITE DE BORDEAUX ,  CENTRE HOSPITALIER UNIVERSITAIRE DE BORDEAUX

Inventor： ANDRIQUE, Laetitia ,  CHEVRET, Edith ,  MERLIO, Jean-Philippe ,  POGLIO, Sandrine

IPC: C12N5/0783 ,  A01K67/027

CPC classification number: C12N5/0636 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2227/105 ,  A01K2267/0331 ,  C12N5/0693

Abstract: L'invention concerne un modèle animal de lymphome cutané primitif ainsi que des méthodes employant ce modèle pour évaluer les propriétés biologiques et génétiques des cellules tumorales de lymphome cutané primitif, ainsi que pour identifier ou évaluer l'efficacité d'un traitement contre un lymphome cutané.

Abstract translation: 本发明涉及皮肤淋巴瘤的动物模型。 以及使用该模型评估皮肤淋巴瘤肿瘤细胞的生物学和遗传特性的方法。 原始的，以及确定或评估的有效性 治疗皮肤淋巴瘤。

公开(公告)号：WO2016181114A1

公开(公告)日：2016-11-17

申请号：PCT/GB2016/051301

申请日：2016-05-06

Applicant: IMAGEN THERAPEUTICS LIMITED

Inventor： BENSON, Roderick ,  GRIFFITHS, Gareth ,  DUTKOG-WOZDZ, Joanna

IPC: C12N5/09 ,  G01N33/50

CPC classification number: C12N5/0693 ,  C12N2500/25 ,  C12N2500/84 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/39 ,  G01N33/5011

Abstract: This present invention relates to a method of culturing cells, wherein the cell culture media comprises a body fluid preferably derived from the same species as the cells being cultured. The body fluid may be derived from the same subject as the cells being cultured, thereby providing a reflection of the in vivo cellular environment. The invention also relates to a media for use in culturing cells in vitro. The cells may be cancer cells.

Abstract translation: 本发明涉及一种培养细胞的方法，其中细胞培养基包含优选源自与培养细胞相同种类的体液。 体液可以衍生自与培养的细胞相同的受试者，从而提供体内细胞环境的反映。 本发明还涉及用于体外培养细胞的培养基。 细胞可能是癌细胞。

公开(公告)号：WO2016154082A2

公开(公告)日：2016-09-29

申请号：PCT/US2016/023345

申请日：2016-03-21

Applicant: TANG, Yao

Inventor： TANG, Yao

IPC: C12N5/09

CPC classification number: C12N5/0694 ,  C12N5/0693 ,  C12N2500/84 ,  C12N2503/02 ,  C12N2509/00 ,  C12N2509/10 ,  C12N2513/00 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

Abstract: The present invention provides methods for culturing primary cells and tissues from a subject in the presence of the subject's own serum, ascites or pleural effusion fluid. Methods of treating cancer, and screening for the effectiveness or toxicity of drugs are also provided herein.

Abstract translation: 本发明提供了在受试者自己的血清，腹水或胸腔积液的存在下从受试者培养原代细胞和组织的方法。 本文还提供了治疗癌症的方法和筛选药物的有效性或毒性。

公开(公告)号：WO2016090361A1

公开(公告)日：2016-06-09

申请号：PCT/US2015/064271

申请日：2015-12-07

Applicant: AKINA, INC. (AN INDIANA (US) CORP) ,  GARNER, John, Solomon ,  SKIDMORE, Sarah, Michelle ,  HADAR, Justin, Charles ,  HAN, Burnsoo ,  PARK, Kinam

Inventor： GARNER, John, Solomon ,  SKIDMORE, Sarah, Michelle ,  HADAR, Justin, Charles ,  HAN, Burnsoo ,  PARK, Kinam

IPC: C08J3/075

CPC classification number: C12N5/0062 ,  C08B3/10 ,  C08F126/06 ,  C08F220/54 ,  C08G18/4825 ,  C08G18/7671 ,  C08G2220/00 ,  C12N5/0068 ,  C12N5/0693 ,  C12N2513/00 ,  C12N2533/20 ,  C12N2533/30 ,  C12N2533/78 ,  C12N2537/00 ,  C12N2539/00

Abstract: The present invention provides the use of selected thermogelling polymers for the purpose of growing tumor spheroids. The invention provides a thermogelling platform comprising a synthetic polymer which, when seeded with cancer cells, induces the cells to grow into a natural spheroidal pattern forming a tumor spheroid. After accomplishing this in about 3-10 days, the gel washes away, leaving behind the spheroids.

Abstract translation: 本发明提供了选择的热凝胶聚合物用于生长肿瘤球体的用途。 本发明提供了一种包含合成聚合物的热凝胶平台，当合成聚合物与癌细胞接触时，诱导细胞生长成形成肿瘤球体的天然球形图案。 大约3-10天之后完成，凝胶洗去，留下球体。

公开(公告)号：WO2016049641A1

公开(公告)日：2016-03-31

申请号：PCT/US2015/052682

申请日：2015-09-28

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： KRUMMEL, Matthew ,  BROZ, Miranda ,  WOLF, Denise ,  POLLACK, Joshua ,  BINNEWIES, Mikhail

IPC: A61K39/395

CPC classification number: C07K16/2803 ,  A61K2039/505 ,  C07K16/243 ,  C07K16/2818 ,  C07K16/2863 ,  C07K16/30 ,  C07K16/3046 ,  C07K16/3053 ,  C07K2317/73 ,  C07K2317/76 ,  C12N5/0626 ,  C12N5/0639 ,  C12N5/0645 ,  C12N5/0693 ,  G01N33/56966

Abstract: Provided herein are methods and compositions for enhancing an immune response and/or for the treatment of an immune-related condition in an individual, e.g., cancer, comprising killing, disabling, or depleting non-stimulatory myeloid cells using an antigen binding protein such as an antibody or antigen binding fragment thereof.

Abstract translation: 本文提供用于增强免疫应答和/或用于治疗个体（例如癌症）中的免疫相关病症的方法和组合物，其包括使用抗原结合蛋白例如杀死，致残或消耗非刺激性骨髓细胞， 抗体或其抗原结合片段。

公开(公告)号：WO2016048299A1

公开(公告)日：2016-03-31

申请号：PCT/US2014/057173

申请日：2014-09-24

Applicant: NATIONAL TAIWAN UNIVERSITY ,  WO, Andrew Man, Chung

Inventor： YANG, Pan-chyr ,  CHEN, Huei, Wen ,  CHEN, Wan-jiun

IPC: C12N5/095 ,  C12N5/09 ,  C12N5/071

CPC classification number: C12N5/0695 ,  C12N5/0693 ,  C12N2501/605 ,  C12N2502/30 ,  G01N33/5017 ,  G01N33/574 ,  G01N2800/52

Abstract: An in vitro co-culture system comprising cancer-associated fibroblasts (CAFs) and cancer cells for producing and maintaining cancer stem cells and uses thereof for identifying agents capable of reducing cancer cell stemness. Also disclosed herein are a paracrine network through which CAFs facilitate production and/or maintenance of cancer stem cells and the use of components of such a paracrine network for prognosis purposes and for identifying cancer patients who are likely to respond to certain treatment.

Abstract translation: 包含癌相关成纤维细胞（CAF）和用于产生和维持癌症干细胞的癌细胞的体外共培养系统及其用于鉴定能够降低癌细胞干性的药剂的用途。 本文还公开了旁分泌网络，其中CAF促进癌症干细胞的生产和/或维持以及这种旁分泌网络的组分用于预后目的并鉴定可能对某些治疗作出反应的癌症患者。

公开(公告)号：WO2015179657A1

公开(公告)日：2015-11-26

申请号：PCT/US2015/031996

申请日：2015-05-21

Applicant: LU, Wuyuan ,  WITZ, Isaac ,  MAMAN, Shelly ,  UNIVERSITY OF MARYLAND, BALTIMORE ,  RAMOT AT TEL-AVIV UNIVERSITY LTD.

Inventor： LU, Wuyuan ,  WITZ, Isaac ,  MAMAN, Shelly

IPC: G01N33/68 ,  G01N33/53 ,  G01N30/22 ,  G01N30/72 ,  A61K38/42 ,  A61P35/00

CPC classification number: A61K38/42 ,  A61K38/16 ,  A61K38/1709 ,  A61K47/48307 ,  C12N5/0693 ,  G01N30/22 ,  G01N30/72 ,  G01N30/88 ,  G01N33/53 ,  G01N33/57488 ,  G01N33/68 ,  G01N33/721 ,  G01N2030/8813

Abstract: The beta 2 subunit of mouse hemoglobin (HBB2) has been identified as soluble factor from mouse lungs that exhibits cytostatic/cytotoxic activity against neuroblastoma lung micrometastases. The beta subunit of human hemoglobin (HBB) has been found to have similar activity. Methods of using these proteins and fragments thereof in the treatment of cancer and inhibition of metastasis are provided, along with methods of screening a subject for micrometastases by detecting HBB in a biological sample.

Abstract translation: 小鼠血红蛋白（HBB2）的β2亚基已经被鉴定为对小鼠肺的可溶性因子，其对神经母细胞瘤肺微转移具有细胞抑制/细胞毒性活性。 已发现人血红蛋白（HBB）的β亚基具有相似的活性。 提供了将这些蛋白质及其片段用于治疗癌症和抑制转移的方法，以及通过检测生物样品中的HBB来筛选受试者的微转移的方法。