公开(公告)号：WO2019067681A1

公开(公告)日：2019-04-04

申请号：PCT/US2018/053061

申请日：2018-09-27

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： CHEN, Samson ,  SCHERER, Axel

IPC: G01N27/327 ,  A61B5/145 ,  C12Q1/00

CPC classification number: A61B5/14865 ,  A61B5/14532 ,  A61B5/1459 ,  C12N9/0004 ,  C12N11/02 ,  C12N11/14 ,  C12Y101/01001 ,  C12Y101/03003 ,  C12Y101/03005 ,  C12Y101/03007 ,  C12Y101/03008 ,  C12Y101/0301 ,  C12Y101/03011 ,  C12Y101/03012 ,  C12Y101/03015 ,  C12Y101/03016 ,  C12Y101/03018 ,  C12Y101/03019 ,  C12Y101/0302 ,  C12Y101/03023 ,  C12Y101/03027 ,  C12Y101/03029 ,  C12Y101/0303 ,  C12Y101/03037 ,  C12Y101/03038 ,  C12Y101/0304 ,  C12Y101/03041 ,  G01N27/3272

Abstract: A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.