公开(公告)号：WO2022016166A2

公开(公告)日：2022-01-20

申请号：PCT/US2021/070654

申请日：2021-06-02

申请人： THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES

发明人： SINGEC, Ilyas ,  SLAMECKA, Jaroslav ,  SIMEONOV, Anton ,  DENG, Tao

IPC分类号： C12N5/073 ,  G01N33/48 ,  C12N2500/02 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/13 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/392 ,  C12N2501/415 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2533/50 ,  C12N5/0605

摘要： Methods for generating in culture of cells resembling mammalian trophectoderm-lineage cells from mammalian pluripotent stem cells are provided, along with the related compositions.

公开(公告)号：WO2021247760A1

公开(公告)日：2021-12-09

申请号：PCT/US2021/035527

申请日：2021-06-02

申请人： THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES

发明人： SINGEC, Ilyas ,  SLAMECKA, Jaroslav ,  SIMEONOV, Anton ,  DENG, Tao

IPC分类号： C12N5/073 ,  C12N2500/02 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/13 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/392 ,  C12N2501/415 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2533/50 ,  C12N5/0605 ,  G01N33/48

摘要： Methods for generating in culture of cells resembling mammalian trophectoderm-lineage cells from mammalian pluripotent stem cells are provided, along with the related compositions.

公开(公告)号：WO2023060083A1

公开(公告)日：2023-04-13

申请号：PCT/US2022/077536

申请日：2022-10-04

申请人： UNIVERSITY OF FLORIDA RESEARCH FOUNDATION

发明人： ANGELINI, Thomas Ettor ,  ABRAHAN, Carolina Elizabeth ,  CHISOLM, Steven ,  DOBSON, Jon P. ,  MATHEWS, Clayton Elwood ,  MCFETRIDGE, Peter S. ,  PALMER, Glyn Daniel ,  SUBRAMANIAM, Vignesh ,  SUMERLIN, Brent S. ,  BAUDY, Andreas ,  HIGGINS, Brett

IPC分类号： C12N5/071 ,  B33Y80/00 ,  B33Y70/00 ,  C12N2501/12 ,  C12N2502/14 ,  C12N2502/28 ,  C12N2503/02 ,  C12N2533/54 ,  C12N2535/10 ,  C12N5/067 ,  C12N5/069 ,  C12N5/0697

摘要： Disclosed herein are compositions, systems, and methods relating to three-dimensional bioprinted tissue models, in particular liver tissue modes. Compositions, systems, and methods as described here provide for improved tissue models, thereby improving their utility for diagnostic, clinical, and research purposes. In certain aspects, tissue models as described herein exhibit gene expression profiles, enzymatic function, and enzyme/protein secretion comparable to in vivo organs.

公开(公告)号：WO2022261471A2

公开(公告)日：2022-12-15

申请号：PCT/US2022/033066

申请日：2022-06-10

申请人： CHILDREN’S HOSPITAL MEDICAL CENTER

发明人： TAKEBE, Takanori ,  REZA, Hasan, Al

IPC分类号： C12N5/071 ,  C12N2500/38 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/2306 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/45 ,  C12N2533/52 ,  C12N2533/90 ,  C12N5/0671 ,  G01N33/48

摘要： Disclosed herein are improved methods of maturing pluripotent stem cell-derived liver organoids with the use of hemoglobin metabolites such as bilirubin. Furthermore, by modulating the amounts of bilirubin used, liver organoids can be used as a model for hyperbilirubinemia. The scalability and tractability of these liver organoids made them excellent targets for drug screening against diseases such as hyperbilirubinemia. Also shown herein is the use of exogenous L-gulonolactone oxidase for improving viability of bilirubin-treated liver organoids.

公开(公告)号：WO2021260049A1

公开(公告)日：2021-12-30

申请号：PCT/EP2021/067221

申请日：2021-06-23

申请人： GENFIT

发明人： VIDAL, Guillaume ,  WALCZAK, Robert

IPC分类号： C12N5/16 ,  G01N33/50 ,  C12N2500/25 ,  C12N2501/11 ,  C12N2501/12 ,  C12N2502/1157 ,  C12N2502/14 ,  C12N2502/28 ,  C12N2533/90 ,  C12N5/0671

摘要： The present invention relates to methods for preparing in vitro models of nonalcoholic steatohepatitis.