公开(公告)号：WO2022242285A1

公开(公告)日：2022-11-24

申请号：PCT/CN2022/081234

申请日：2022-03-16

Applicant: 广州凯普医药科技有限公司 ,  广东凯普生物科技股份有限公司

Inventor： 郑焱 ,  马佳艳 ,  丰晓威 ,  刘梦羽 ,  陈佩璇 ,  管秩生 ,  谢龙旭 ,  葛毅媛

IPC: C12N5/073 ,  G01N15/14 ,  C12N2509/10 ,  C12N5/0605

Abstract: 提供了一种从孕妇宫颈脱落细胞中分离胎盘滋养层细胞的方法，所述方法基于特定的滋养层细胞表面或胞内表达的特异性抗原及组合，并利用设计的微流控分选芯片或流式细胞仪，对胎盘滋养层样本的细胞悬浮液进行细胞分选，获得分离纯化的胎盘滋养层细胞。与传统方法相比，所述方法具有无创获取标本，特异性好，取材时间较早，导致感染及流产的风险低的优势，能实现同时对多个抗原进行同步标记和特征荧光信号的识别和分选，准确性得到较大提升，检测结果又具有更高的可信度和更广的覆盖范围。与现有磁珠法技术相比，所述方法获得细胞数量多，特异性好，灵敏性好，能够应用于多种检测用途和应用场景。

公开(公告)号：WO2021146442A1

公开(公告)日：2021-07-22

申请号：PCT/US2021/013464

申请日：2021-01-14

Applicant: MUSCULOSKELETAL TRANSPLANT FOUNDATION

Inventor： LONG, Marc ,  SEMLER, Eric ,  CITRO, Mat ,  CHNARI, Evangelia

IPC: A61L27/36 ,  A61L27/40 ,  A61K35/32 ,  C12N5/071 ,  C12N5/073 ,  C12N5/077 ,  A61K45/06 ,  A61L27/3608 ,  A61L27/3612 ,  A61L27/3654 ,  C12N2533/90 ,  C12N2533/92 ,  C12N5/0605 ,  C12N5/0654 ,  C12N5/0655 ,  C12N5/0697

Abstract: Multiple component grafts are provided for treatment of tissue defects and comprise two or more components, each of which is a tissue-derived matrix and at least two of which are derived from different types of tissue. For example, a first component may be a matrix derived from cartilage tissue such as cartilage fibers with or without viable cells, cartilage particles with or without viable cells, or combinations of any two or more such cartilage-derived matrices. A second component may be a matrix derived from bone tissue such as mineralized or demineralized cortical bone fibers, viable cancellous bone matrix (e.g., cryopreserved or lyophilized chips, particulates, powder, sheets, putty, flowable fluid, etc.), demineralized or demineralized cancellous bone matrix (chips, particulates, powder, sheets, putty, flowable fluid, etc.), or combinations of any two or more of such bone-derived matrices. Also provided are methods for making and using such multiple component grafts.

公开(公告)号：WO2021259909A1

公开(公告)日：2021-12-30

申请号：PCT/EP2021/066945

申请日：2021-06-22

Applicant: MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V.

Inventor： SATHYANARAYANAN, Anusha ,  BEDZHOV, Ivan

IPC: C12N5/073 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/727 ,  C12N5/0605

Abstract: The present invention relates to a method for preparing trophectoderm-like cells comprising culturing a blastocyst, extraembryonic endoderm stem (XEN) cells and/or XEN-like cells in a medium comprising a glycogen synthase kinase 3 (GSK-3) inhibitor.

公开(公告)号：WO2021128763A1

公开(公告)日：2021-07-01

申请号：PCT/CN2020/096297

申请日：2020-06-16

Applicant: 北京光捷扬基健康科技有限公司

Inventor： 陈伟 ,  万美蓉 ,  高云 ,  杨若霖 ,  时晰

IPC: A61L27/36 ,  A61L27/60 ,  A61L27/50 ,  A61L27/54 ,  A61L2430/40 ,  A61L27/3604 ,  A61L27/3687 ,  C12N5/0605

Abstract: 本发明涉及医用材料技术领域，具体涉及一种胎盘组织工程去免疫原皮肤支架的制备方法。本发明的胎盘组织工程去免疫原皮肤支架的制备方法，包括以下步骤：1）从胎盘中无菌分离出胎盘膜；2）将分离出的胎盘膜清洗后置于抗生素溶液中浸泡，进行消毒处理；3）反复用无菌蒸馏水冲洗浸泡胎盘；4）将消毒后的胎盘膜用不同浓度的乙醇-生理盐水溶液中梯度脱水，去除蛋白，灭活免疫原；5）将脱水后的胎盘膜浸泡于乙醇中，浸泡后取出风干，制成皮肤支架。本发明能够实现简便，快速，高效，大规模制备医用人工皮肤的目的，且易于保存，便于运输。

公开(公告)号：WO2021237762A1

公开(公告)日：2021-12-02

申请号：PCT/CN2020/093716

申请日：2020-06-01

Applicant: 青岛瑞思德生物科技有限公司

Inventor： 张炳强 ,  陈梦梦

IPC: C12N5/0775 ,  A61K35/28 ,  C12N5/00 ,  A01N1/0221 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/21 ,  C12N2501/231 ,  C12N2501/235 ,  C12N2501/39 ,  C12N2501/405 ,  C12N2501/999 ,  C12N5/0605 ,  C12N5/0663 ,  C12N5/0665 ,  C12N5/0668 ,  C40B50/06

Abstract: 本发明公开了一种间充质干细胞体外筛选、激活、扩增、冻存及其细胞库建立的方法。该方法包括：利用间充质干细胞专用原代筛选培养基进行第一阶段筛选培养，得到纯化的间充质干细胞；利用间充质干细胞专用激活扩增培养基将纯化的间充质干细胞进行第二阶段激活和大规模扩增培养，获得大量激活功能的间充质干细胞；利用间充质干细胞专用冻存液冻存干细胞，并按ABO/RH分型和HLA分型进行保存，建立可供检索的信息档案，构建间充质干细胞库。

公开(公告)号：WO2021202771A2

公开(公告)日：2021-10-07

申请号：PCT/US2021/025221

申请日：2021-03-31

Applicant: YOUNG, Bruce K. ,  CHAN, Michael K.

Inventor： YOUNG, Bruce K. ,  CHAN, Michael K.

IPC: A61K35/50 ,  A61K35/545 ,  C12N5/0605

Abstract: Amniotic fluid stem cell clones are provided. Also provided are pharmaceutical compositions comprising the amniotic fluid stem cell clones, methods of generating the amniotic fluid stem cell clones, and methods of using the amniotic fluid stem cell clones, including for treating subjects and for bioassays.

公开(公告)号：WO2022016166A2

公开(公告)日：2022-01-20

申请号：PCT/US2021/070654

申请日：2021-06-02

Applicant: THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES

Inventor： SINGEC, Ilyas ,  SLAMECKA, Jaroslav ,  SIMEONOV, Anton ,  DENG, Tao

IPC: C12N5/073 ,  G01N33/48 ,  C12N2500/02 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/13 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/392 ,  C12N2501/415 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2533/50 ,  C12N5/0605

Abstract: Methods for generating in culture of cells resembling mammalian trophectoderm-lineage cells from mammalian pluripotent stem cells are provided, along with the related compositions.

公开(公告)号：WO2021247760A1

公开(公告)日：2021-12-09

申请号：PCT/US2021/035527

申请日：2021-06-02

Applicant: THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES

Inventor： SINGEC, Ilyas ,  SLAMECKA, Jaroslav ,  SIMEONOV, Anton ,  DENG, Tao

IPC: C12N5/073 ,  C12N2500/02 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/13 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/392 ,  C12N2501/415 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2533/50 ,  C12N5/0605 ,  G01N33/48

Abstract: Methods for generating in culture of cells resembling mammalian trophectoderm-lineage cells from mammalian pluripotent stem cells are provided, along with the related compositions.