公开(公告)号：WO2022270684A1

公开(公告)日：2022-12-29

申请号：PCT/KR2021/012654

申请日：2021-09-16

Applicant: 주식회사 세이브앤코

Inventor： 박지원 ,  김정민

IPC: C12P17/06 ,  A61K8/9789 ,  A61Q19/00 ,  A61K2800/85 ,  A61K8/498 ,  C12P17/162 ,  C12R2001/25

Abstract: 본 발명은 크랜베리 및 유산균을 활용한 저분자량화 프로안토시아니딘 추출 방법에 대한 것으로, 보다 구체적으로 (S1) 크랜베리에서 종균을 배양하고 추출하는 단계, (S2) 대사과정을 조정하여 당류의 대사를 강화하는 단계, (S3) 대사과정이 조정된 종균을 추출하여 배양하는 단계, (S4) 배양된 종균에 크랜베리, 증류수 및 당류를 혼합하여 발효하는 단계, (S5) 발효된 혼합물로부터 비결합형 폴리페놀을 추출하는 단계를 포함할 수 있다.

公开(公告)号：WO2022109736A1

公开(公告)日：2022-06-02

申请号：PCT/CA2021/051682

申请日：2021-11-24

Applicant: ROTHSTEIN, Steven ,  AKHTAR, Tariq ,  CASARETTO, Jose ,  PERRIN, Colby ,  VAN GELDER, Kristen

Inventor： ROTHSTEIN, Steven ,  AKHTAR, Tariq ,  CASARETTO, Jose ,  PERRIN, Colby ,  VAN GELDER, Kristen

IPC: C12P17/06 ,  A61K8/49 ,  A61K31/352 ,  C07D311/30 ,  C12N9/10 ,  C12N15/54 ,  C12N15/63 ,  C12P7/22

Abstract: Described herein is a polypeptide encoding a prenyltransferase for prenylating a polyphenol and a polypeptide encoding an O-methyltransferase for methylating a polyphenol. For example, the polypeptide comprises or consists of the sequence of SEQ ID NO: 1, 2, 3, 4, 5, and/or 6, and/or a polypeptide listed in Table 1, and/or SEQ ID NO: 7-30, or a variant thereof having at least 80% sequence identity to SEQ ID NO: 1, 2, 3, 4, 5, and/or 6, and/or the polypeptide listed in Table 1, and/or SEQ ID NO: 7-30, or a fragment of the polypeptide or the variant thereof.

公开(公告)号：WO2022101306A1

公开(公告)日：2022-05-19

申请号：PCT/EP2021/081309

申请日：2021-11-11

Applicant: TECHNISCHE UNIVERSITÄT DARMSTADT

Inventor： GEISSLER, Marcus ,  NIEDERREITER, Lisa ,  WARZECHA, Heribert

IPC: C12P7/22 ,  C12P7/42 ,  C12P17/06 ,  C12N9/02

Abstract: The present invention relates to a process for the production of a compound of Formula (I), wherein the process comprises the step of reacting a plant extract having prenyltransferase activity with a compound of Formula (II) and geranyl pyrophosphate (GPP) having Formula (III) to produce the compound of Formula (I), wherein the plant extract is not a plant extract obtainable from a plant that naturally produces cannabinoids/phytocannabinoids. Accordingly, the present invention relates to a process for the production of a compound of Formula (I), wherein the process comprises the step of reacting a plant extract having prenyltransferase activity with a compound of Formula (II) and geranyl pyrophosphate (GPP) having Formula (III) to produce the compound of Formula (I), wherein the plant extract is not Cannabis sativa plant extract. Further, the present invention relates to the process for the production of a compound of Formula (IV), wherein the process comprises producing a compound of Formula (I) by the process as described herein and converting the compound of Formula (I) to the compound of Formula (IV) in the presence of the tetrahydrocannabinolic acid synthase (THCAS; EC 1.21.3.7). The present invention also relates to a process for the production of a compound of Formula (V) wherein the process comprises producing a compound of Formula (I) by the process as described herein and converting the compound of Formula (I) to a compound of Formula (V) in the presence of the THCAS (EC 1.21.3.7), cannabidiolic acid synthase (CBDAS; EC 1.21.3.8) and/or cannabichromenic acid synthase (CBCAS; EC 1.3.3.-). Further, the present invention relates to a process for the production of a compound of Formula (VI) wherein the process comprises producing a compound of Formula (I) by the process as described herein and converting the compound of Formula (I) to a compound of Formula (VI) in the presence of the CBDAS (EC 1.21.3.8).

公开(公告)号：WO2022016254A1

公开(公告)日：2022-01-27

申请号：PCT/CA2021/050894

申请日：2021-06-29

Applicant: HYASYNTH BIOLOGICALS INC.

Inventor： CAMPBELL, Alexander ,  PALYS, Sylvester

IPC: C12N15/54 ,  C07C65/05 ,  C12N15/52 ,  C12N15/63 ,  C12N9/00 ,  C12N9/10 ,  C12P17/06 ,  C12P19/56 ,  C12P19/60 ,  C12P7/40 ,  C12P7/42

Abstract: The present disclosure relates generally to methods and cells for the production of substituted phytocannabinoids or substituted phytocannabinoid precursors in host cells that produce the phytocannabinoid or the phytocannabinoid precursor. Methods are described which comprise transforming host cells with a sequence encoding an enzyme for derivatizing the phytocannabinoid or precursor with a substituent, such as O-methyl, glycosyl, or halogen. The transformed cells are cultured to produce substituted phytocannabinoids or substituted phytocannabinoid precursors.

公开(公告)号：WO2021163679A1

公开(公告)日：2021-08-19

申请号：PCT/US2021/018158

申请日：2021-02-16

Applicant: OHIO STATE INNOVATION FOUNDATION

Inventor： ZHU, Xiaoyi ,  STRAATHOF, Nicole ,  MIYAGUSUKU-CRUZADO, Gonzalo ,  GIUSTI, M. Monica ,  CANO, Israel Garcia ,  JIMENEZ-FLORES, Rafael

IPC: C12P17/06

Abstract: The present disclosure provides methods for the rapid synthesis of pyranoanthocyanins.

公开(公告)号：WO2021092689A1

公开(公告)日：2021-05-20

申请号：PCT/CA2020/051537

申请日：2020-11-12

Applicant: ALGAE-C INC.

Inventor： SHARMA, Nikunj ,  DESGAGNÉ-PENIX, Isabel

IPC: C12N13/00 ,  C12N1/00 ,  C12N1/13 ,  C12N1/21 ,  C12N15/52 ,  C12N15/53 ,  C12N15/54 ,  C12N15/60 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12P1/00 ,  C12P17/06 ,  C12P7/40 ,  C12P7/42

Abstract: A method of culturing a photosynthetic microorganism comprising culturing the photosynthetic microorganism under a first light spectrum for a first period of time and under a second light spectrum for a second period of time. Further described is a method of culturing a photosynthetic microorganism capable of producing at least one biosynthetic product in a culture medium comprising culturing the photosynthetic microorganism under a first light spectrum for a first period of time and under a second light spectrum for a second period of time, wherein the culture medium comprises a 2,4-dihydroxy-6-alkylbenzoic acid or a 2,4-dihydroxy-6-alkylbenzoate.

公开(公告)号：WO2021058115A1

公开(公告)日：2021-04-01

申请号：PCT/EP2019/076265

申请日：2019-09-27

Applicant: SYMRISE AG

Inventor： GEISSLER, Torsten ,  LEY, Jakob Peter ,  ZIRPEL, Bastian

IPC: C12P7/26 ,  A23L27/30 ,  C12P17/06

Abstract: The present invention relates to a biocatalytical method for manufacturing of homoeriodictyol dihydrochalcone and/or hesperetin dihydrochalcone by providing at least one first biocatalyst system for the hydroxylation of phloretin and/or its glycosides as well as at least one second biocatalyst for the methylation of 3-hydroxyphloretin. Further disclosed are microorganisms capable of producing such biocatalysts as well as sequences encoding the biocatalysts. Furthermore, the present invention relates to the use of a mixture obtained by a method as disclosed in the present invention and to specific compositions suitable as sweetness enhancers and/or flavouring agents.

公开(公告)号：WO2021034847A1

公开(公告)日：2021-02-25

申请号：PCT/US2020/046837

申请日：2020-08-18

Applicant: BIOMEDICAN, INC.

Inventor： MIKHEEV, Maxim

IPC: C12N9/02 ,  C12N9/10 ,  C12N15/52 ,  C12P17/06

Abstract: The present disclosure relates to the production of cannabinoids in either recombinant microorganism or in cell-free systems using a combination of enzymes, including but not limited to a PKS enzyme, a npgA enzyme, a cs-OLAS-1, a pp-DVAS-1, a cs-HEX-1 and/or Butiryl synthase.

公开(公告)号：WO2020165882A2

公开(公告)日：2020-08-20

申请号：PCT/IB2020/051457

申请日：2020-02-21

Applicant: 한국화학연구원

Inventor： 차현길 ,  주정찬 ,  김희택 ,  김경안 ,  박제영 ,  강명종 ,  황성연 ,  오동엽 ,  이민우

IPC: C07D309/38 ,  C12N15/70 ,  C12N9/02 ,  C12P17/06

Abstract: 본 발명은 고순도 2-피론-4,6-디카르복실산 제조방법 및 그를 위한 중간체 제조방법으로; 1) PET로부터 TPA를 제조하는 단계; 2) TPA를 재조합 균주와 반응시켜 PDC를 생성하는 단계; 및 3) 제조된 PDC를 고순도로 정제하는 단계를 포함한다. 본 발명에 의한 바이오 매스 유래 SiO2 촉매, 그 제조방법, 및 이를 이용한 PET의 TPA 분해방법을 이용하면, 종래의 중성 조건하에서 PET 가수분해 속도 보다 빠른 시간에 고순도 테레프탈 산(TPA)의 제조가 가능하며, 제조된 테레프탈 산은 다시 폴리에스테르 섬유의 원료 등으로 유용하게 사용할 수 있다. 본 발명은 TphB 효소, TphAabc 효소, LigAB 효소 및 LigC 효소를 코딩하는 뉴클레오티드 서열이 도입된, 2-피론-4,6-디카복실산(PDC) 생산용 재조합 균주 및 상기 PDC 생산용 재조합 균주와 기질을 반응시키는 단계를 포함하는 PDC의 생산방법에 관한 것이다. 본 발명은 공업적으로 제조되거나 상업적으로 입수가능한 조질(crude) 2-피론-4,6-디카르복실산 또는 이의 소듐염에서, NMR 및/또는 TLC 분석법으로 확인되지만 제거 및 정제가 곤란한 불순물을, 아세톤을 첨가하여 석출시켜 제거하고, 결과된 용액을 유기용매로 추출 및 농축하고, 결과된 농축물을 컬럼 크로마토그래피에서 에틸아세테이트와 헥산의 혼합용매로 용리함으로써, 2-피론-4,6-디카르복실산을 고순도로 정제하는 방법을 제공한다.

公开(公告)号：WO2020163378A1

公开(公告)日：2020-08-13

申请号：PCT/US2020/016642

申请日：2020-02-04

Applicant: TRAIT BIOSCIENCES, INC.

Inventor： SAYRE, Richard T. ,  ZIDENGA, Tawanda ,  LEBRUN, Erick Scott ,  TRAVERS, Timothy, S. ,  STAMPS, Jennifer

IPC: C12P17/06 ,  C12P7/42

Abstract: The inventive technology includes novel systems, methods, and compositions for the generation of water-soluble short-chain fatty acid phenolic compounds, preferably cannabinoids, terpenes, and other volatile compounds produced in Cannabis and other cannabinoid producing plants. In particular, the inventive technology includes novel systems and methods to solubilize short-chain fatty acid phenolic compounds, such as cannabinoids, via binding to a water soluble and readily digested carrier protein.