公开(公告)号：WO2023018232A1

公开(公告)日：2023-02-16

申请号：PCT/KR2022/011953

申请日：2022-08-10

Applicant: 주식회사 에스엠엘제니트리

Inventor： 정요한 ,  이정주 ,  박소현 ,  황경아 ,  안지훈

IPC: C12Q1/6811 ,  C12Q1/6886 ,  C12Q1/689 ,  C12Q1/70

Abstract: 생물학적 샘플 내 핵산 분자의 검출을 위한 비드 복합체 및 이를 이용한 핵산을 검출하는 방법에 관한 것으로, 일 양상에 따른 비드 복합체에 의하면, 생물학적 샘플 내에서 효과적으로 표적 핵산 분자를 분리, 추출, 및 검출할 수 있고, 생물학적 샘플 내에 표적 핵산 분자가 존재하는지의 여부를 분석함으로써, 분석의 민감도를 향상시킬 수 있는 효과가 있다.

公开(公告)号：WO2022226372A1

公开(公告)日：2022-10-27

申请号：PCT/US2022/026043

申请日：2022-04-22

Applicant: ULTIMA GENOMICS, INC.

Inventor： OBERSTRASS, Florian ,  DOVER, Ron Saar ,  SHIPONY, Zohar ,  ALMOGY, Gilad ,  MAZUR, Daniel

IPC: C12Q1/6869 ,  C12Q1/6811 ,  C12Q1/6837 ,  C12N15/10 ,  C40B20/04 ,  C12N15/115

Abstract: Provided herein are methods, systems, compositions, and kits that can determine spatial information between a plurality of analytes in a sample by tagging such analytes with tags, where identities and/or locations of such tags are previously unknown prior to tagging. Each of a plurality of analyte sequences may be tagged with multiple spatial tags, such that each analyte sequence is associated with a set of two or more spatial tags. The sets of spatial tags may be analyzed to generate a map of analyte sequences. The map may comprise information about the respective absolute positions of each of a set of sequences with respect to a reference sequence. The map may comprise information about the respective probability cloud (or likely location) of each of a set of sequences with respect to a reference sequence.

公开(公告)号：WO2022187867A1

公开(公告)日：2022-09-09

申请号：PCT/US2022/070998

申请日：2022-03-07

Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

Inventor： SANTELLA, Regina ,  ZHOU, Hui ,  WU, Hui Chen ,  ZHANG, Zhiguo

IPC: C12Q1/6811 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6886 ,  C12Q1/6827

Abstract: Disclosed herein are methods for detecting methylation in cell-free polynucleotides and methods for detecting the presence of cancer in a subject.

公开(公告)号：WO2022159035A1

公开(公告)日：2022-07-28

申请号：PCT/SG2022/050021

申请日：2022-01-20

Applicant: NATIONAL UNIVERSITY OF SINGAPORE ,  SINGAPORE HEALTH SERVICES PTE LTD

Inventor： CHEOW, Lih Feng ,  ......., Elsie Cheruba ,  TAN, Bee Huat Iain ,  CHONG, Qingqing Dawn

IPC: C12Q1/6811 ,  C12Q1/6869

Abstract: The present invention is directed to a method using heat denaturation of DNA fragments having low GC content to enrich for CpG regions that can be enriched by adapter-ligation, 5 subjected to bisulfite conversion, sequenced and analysed, for example, to detect cancer.

公开(公告)号：WO2022115539A2

公开(公告)日：2022-06-02

申请号：PCT/US2021/060735

申请日：2021-11-24

Applicant: WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH

Inventor： YOUNG, Richard A. ,  HENNINGER, Jonathan E. ,  OKSUZ, Ozgur

IPC: C12N15/113 ,  A61K31/7105 ,  A61K31/711 ,  C12Q1/6811 ,  C12N15/63 ,  C12N2310/14

Abstract: Disclosed herein are methods of modulating condensate dependent transcription of a gene by modulating condensate associated nucleic acids, including enhancer RNA and other regulatory RNA. Also disclosed are methods of screening for agents that modulate condensate dependent transcription of a gene by modulating condensate associated nucleic acids.

公开(公告)号：WO2022070185A1

公开(公告)日：2022-04-07

申请号：PCT/IL2021/051172

申请日：2021-09-29

Applicant: TECHNION RESEARCH & DEVELOPMENT FOUNDATION LIMITED

Inventor： AMIT, Roee ,  KATZ, Noa

IPC: C12Q1/6811 ,  G16B15/00

Abstract: Synthetic RNA molecules comprising at least two first RNA-binding protein (RBP)- binding motifs, at least two second RBP-binding motifs and at least two third RBP-binding motifs, wherein the at least two first RBP-binding motifs bind the same first RBP and comprise non-identical sequences are provided. Synthetic RNA molecules comprising at least two RBP-binding motifs, a regulatory element and an open reading frame wherein the RBP-binding motifs individually repress translation and cooperatively enhance translation of the open reading frame are also provided. Methods employing machine learning models to determine variant sequence binding to RBPs are also provided.

公开(公告)号：WO2021168464A1

公开(公告)日：2021-08-26

申请号：PCT/US2021/019182

申请日：2021-02-23

Applicant: THE JACKSON LABORATORY

Inventor： WEI, Chia-Lin ,  NGAN, Chew Yee

IPC: C12N15/11 ,  C12N15/113 ,  C12N15/67 ,  C12Q1/6811 ,  C12Q1/6886 ,  C12N15/635 ,  C12Q1/6869 ,  C12Q2600/136 ,  C12Q2600/158

Abstract: The invention, in part, relates to assessing interactions between gene transcription enhancers and gene transcription repressor, identifying agents that modulate transcription, and use of methods and identified agents to prevent and treat diseases and conditions, such as cancers.

公开(公告)号：WO2021022046A1

公开(公告)日：2021-02-04

申请号：PCT/US2020/044272

申请日：2020-07-30

Applicant: BIOSKRYB, INC. ,  ST. JUDE CHILDREN'S RESEARCH HOSPITAL, INC.

Inventor： WEST, Jay A.A. ,  GAWAD, Charles ,  GONZALES-PENA, Veronica ,  CARTER, Robert ,  NATARAJAN, Sivaraman

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6811

Abstract: Provided herein are compositions and methods for accurate and scalable Primary Template-Directed Amplification (PTA) nucleic acid amplification and sequencing methods, and their applications for mutational analysis in research, diagnostics, and treatment. Such methods and compositions facilitate highly accurate amplification of target (or ''template") nucleic acids, which increases accuracy and sensitivity of downstream applications, such as Next-Generation Sequencing.

公开(公告)号：WO2020256639A1

公开(公告)日：2020-12-24

申请号：PCT/SG2020/050342

申请日：2020-06-18

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH ,  TAN TOCK SENG HOSPITAL PTE LTD

Inventor： HIRAO, Ichiro ,  MATSUNAGA, Ken-ichiro ,  HIRAO, Michiko ,  SUN, William ,  LEO, Yee Sin

IPC: C12N15/115 ,  G01N33/569 ,  C12Q1/6811

Abstract: There is provided an aptamer for dengue virus, optionally an aptamer for dengue virus NS1 protein. The aptamer comprising at least one unnatural base, wherein the unnatural base may be 7-(2thienyl)imidazo[4,5-b]pyridine (Ds), pyrrole2-carbaldehyde (Pa) or 2-nitro-4-propynylpyrrole (Px). The aptamers of the invention may be used to identify a dengue infection in a subject. Also provided are mixtures and kits comprising the aptamer.

公开(公告)号：WO2020161187A1

公开(公告)日：2020-08-13

申请号：PCT/EP2020/052868

申请日：2020-02-05

Applicant: MOLIGO TECHNOLOGIES AB

Inventor： DUCANI, Cosimo ,  HOGBERG, Bjorn

IPC: C12Q1/6811 ,  C12Q1/6853 ,  C12Q1/6841

Abstract: The present invention relates to functionalised single stranded oligonucleotides and in particular to a method for producing functionalised single stranded oligonucleotides comprising: (a) providing a circular DNA molecule comprising an oligonucleotide sequence bordered by cleavage domains; (b) performing a rolling circle amplification (RCA) reaction with the circular DNA molecule of (a) as a template and one or more functionalised nucleotides (dNTPs); and (c) enzymatically cleaving the product of the RCA reaction at the cleavage domains to release the single stranded functionalised oligonucleotides.