公开(公告)号：WO1999001738A2

公开(公告)日：1999-01-14

申请号：PCT/US1998013785

申请日：1998-07-01

Applicant: UNIVERSITY OF MARYLAND, BALTIMORE

Inventor： UNIVERSITY OF MARYLAND, BALTIMORE ,  KEAY, Susan, K. ,  HISE, Michael, K. ,  WARREN, John, W.

IPC: G01N00/00

CPC classification number: G01N33/6893 ,  G01N33/74 ,  G01N2333/475 ,  G01N2333/485 ,  G01N2800/34 ,  G01N2800/348

Abstract: Interstitial cystitis (IC) is a chronic bladder disease for which the exact etiology is unknown and for which there is no reliably effective treatment. However, it is known that the bladder epithelium is often abnormal in IC. We discovered that normal, adult, human bladder epithelial cells are inhibited from proliferating by an anti-proliferative factor (APF) present in IC urine specimens. Inhibited proliferation may cause epithelial abnormalities characteristic of IC such as ulcerations and multiple tears in the bladder epithelium. We further discovered that levels of heparin binding - epidermal growth factor-like growth factor (HB-EGF), a factor known to be important for epithelial cell proliferation and wound healing in other tissues, are abnormally low in the urine of patients suffering from IC as compared to asymptomatic controls or patients with acute bacterial cystitis. The invention herein is directed to the use of urine levels of HB-EGF as a diagnostic marker for IC.

Abstract translation: 间质性膀胱炎（IC）是一种慢性膀胱疾病，其确切的病因是未知的，并且没有可靠的有效治疗。 然而，已知膀胱上皮在IC中通常是异常的。 我们发现正常，成人，人膀胱上皮细胞被IC尿液标本中存在的抗增殖因子（APF）抑制增殖。 抑制性增殖可能导致IC的特征性上皮异常，如膀胱上皮中的溃疡和多发性泪液。 我们进一步发现肝素结合水平 - 表皮生长因子样生长因子（HB-EGF）是已知对其他组织中的上皮细胞增殖和伤口愈合重要的因子，在患有IC的患者的尿液中异常低 与无症状对照或急性细菌性膀胱炎患者相比。 本发明涉及使用HB-EGF的尿液水平作为IC的诊断标记物。

公开(公告)号：WO1998027415A2

公开(公告)日：1998-06-25

申请号：PCT/US1997023142

申请日：1997-12-04

Applicant: GENERAL ELECTRIC COMPANY

Inventor： GENERAL ELECTRIC COMPANY ,  HU, Hui

IPC: G01N00/00

CPC classification number: A61B6/032 ,  A61B6/027 ,  G06T11/005 ,  G06T2211/416 ,  G06T2211/432 ,  Y10S378/901

Abstract: In a computed tomography imaging system, wherein a longitudinally-unbounded object is oriented with respect to a source of cone beam radiation and a detector array, a method is provided for reconstructing an image of the longitudinally-unbounded object. Initially, relative movement between a longitudinally-unbounded object and the cone beam source is established along at least one circular scan path and at least one linear component. The cone beam is operated to irradiate measurable regions of the object while a prescribed orbit is traversed, to project an image of the longitudinally-unbounded object as cone beam data, onto the detector array. A scan field of view is defined, relative to the cone beam geometry. An error propagation distance in a Z direction is determined for the scan. The definition of the scan field of view is then modified, according to error propagation distance, and a set of image reconstruction data is generated, within the modified field of view, from the circular and linear scan paths.

Abstract translation: 在计算机断层成像系统中，其中纵向无界物体相对于锥形束辐射源和检测器阵列定向，提供了用于重建纵向无界物体的图像的方法。 最初，沿着至少一个圆形扫描路径和至少一个线性分量建立纵向无界物体与锥形束源之间的相对移动。 操作锥形束以在规定的轨道穿过时照射物体的可测量区域，将纵向无界物体的图像作为锥形束数据投影到检测器阵列上。 相对于锥形束几何形状，定义扫描视场。 为扫描确定Z方向的误差传播距离。 然后根据误差传播距离修改扫描视野的定义，并且在修改的视野内，从圆形和线性扫描路径生成一组图像重建数据。

公开(公告)号：WO1997035173A2

公开(公告)日：1997-09-25

申请号：PCT/EP1997001311

申请日：1997-03-15

Applicant: BOEHRINGER MANNHEIM GMBH ,  FÜRST, Otto ,  JÄCK, Thomas ,  WEBER, Peter ,  JANSEN, Paul

Inventor： BOEHRINGER MANNHEIM GMBH

IPC: G01N00/00

CPC classification number: G01N35/1004 ,  B08B3/02 ,  B08B3/04 ,  B08B9/00 ,  Y10T436/114998 ,  Y10T436/119163

Abstract: The invention concerns a device for cleaning pipette needles or stirrers, the device comprising a trough (1) which holds cleaning fluid and whose lower region has a fluid duct (3) for filling and/or emptying purposes and whose upper region has at least one feed pipe (6) which leads into at least one nozzle (23) directed into the trough interior. Advantageously, the device has a cylindrical trough (1) and an insert (20) with nozzles (23) which is screwed into the trough (1). The invention further concerns a method of cleaning pipette needles or stirrers, the material to be washed being introduced into the trough interior and sprayed with a washing fluid. The device can also be used to flush the interior of a pipette needle.

公开(公告)号：WO1997017600A2

公开(公告)日：1997-05-15

申请号：PCT/US1996015356

申请日：1996-09-25

Applicant: INFECTECH, INC.

Inventor： INFECTECH, INC. ,  FELDER, Mitchell, S. ,  OLLAR, Robert-A.

IPC: G01N00/00

CPC classification number: C12Q1/18 ,  Y10S435/81 ,  Y10S435/975

Abstract: A method for determining the sensitivity of at least one nonparaffinophilic microorganism from a specimen obtained from a patient to different antimicrobial agents, and predetermined quantities thereof. The method includes providing at least one receptacle containing an aqueous solution and adjusting the solution to mimic the in vivo clinical conditions of said patient. The method further includes inoculating the solution with the specimen and placing into the receptacle (i) a slide coated with a carbon source and (ii) a predetermined quantity of an antimicrobial agent to be tested. By observing the nonparaffinophilic microorganism growth or lack thereof on the slide, it can be determined whether the predetermined quantity of the antimicrobial agent is effective in inhibiting growth of the nonparaffinophilic microorganism on the slide. An associated apparatus is also disclosed.

Abstract translation: 用于确定从患者获得的样品至不同抗微生物剂的至少一种非嗜碳性微生物的敏感性的方法及其预定量。 该方法包括提供至少一个含有水溶液的容器并调节溶液以模拟所述患者的体内临床状况。 该方法还包括用样品接种溶液并将其放入容器中（i）涂覆有碳源的载玻片和（ii）预定量的待测试的抗微生物剂。 通过观察载玻片上的非嗜嗜性微生物的生长或不存在，可以确定预定量的抗微生物剂是否有效抑制载玻片上的非嗜性微生物的生长。 还公开了一种相关联的装置。

公开(公告)号：WO1997014026A2

公开(公告)日：1997-04-17

申请号：PCT/CA1996000676

申请日：1996-10-10

Applicant: LANSDORP, Peter

IPC: G01N00/00

CPC classification number: C12Q1/6876 ,  C12Q1/6841 ,  C12Q1/6886 ,  C12Q2600/136 ,  C12Q2525/151 ,  C12Q2525/113 ,  C12Q2525/107

Abstract: A method for detecting or quantitating multiple copies of a repeat sequence in a nucleic acid molecule involving treating the nucleic acid molecule with a probe which is a nucleic acid analogue which is capable of hybridizing to the repeat sequence in the nucleic acid molecule and which is labelled with a detectable substance. The nucleic acid molecule is treated with the probe under conditions permitting the probe to hybridize to the repeat sequences in the nucleic acid molecule. Probe hybridized to complementary repeat sequences is identified in the nucleic acid molecule by directly or indirectly detecting the detectable substance. The method is preferably used for quantitating multiple copies of a repeat sequence in a nucleic acid molecule, preferably a telomere or centromere repeat sequence. Novel probes for use in the method of the invention and kits are described.

Abstract translation: 一种用于检测或定量核酸分子中重复序列的多个拷贝的方法，包括用能够与核酸分子中的重复序列杂交的核酸类似物的探针来处理核酸分子，并且被标记 与可检测物质。 在允许探针与核酸分子中的重复序列杂交的条件下用探针处理核酸分子。 通过直接或间接检测可检测物质，在核酸分子中鉴定与互补重复序列杂交的探针。 该方法优选用于在核酸分子，优选端粒或着丝粒重复序列中定量重复序列的多个拷贝。 描述了用于本发明方法和试剂盒的新型探针。

公开(公告)号：WO1997012218A2

公开(公告)日：1997-04-03

申请号：PCT/DE1996001799

申请日：1996-09-18

Applicant: FORSCHUNGSZENTRUM JÜLICH GMBH ,  SCHÖNING, Michael, Josef ,  SCHÜTZ, Stefan ,  WEISSBECKER, Bernhard ,  SCHWARZ, Axel ,  KOHL, Claus-Dieter ,  HUMMEL, Hans, E. ,  KORDOS, Peter ,  LÜTH, Hans

Inventor： FORSCHUNGSZENTRUM JÜLICH GMBH

IPC: G01N00/00

CPC classification number: G01N33/54373

Abstract: The invention pertains to a biosensor with a chemoreceptor for detecting one or more trace components produced by burning. The chemoreceptor is preferably connected to a semiconductor component via an electrolyte. The biosensor can be designed in such a way that only a small number of insect antenna segments, in particular a single such segment, is connected at one end of the chemoreceptor directly or via the electrolyte to the semiconductor component.

Abstract translation: 本发明涉及一种用于检测火灾的一个或多个痕量组分，预期的化学感受器的生物传感器。 在此，化学感受器优选通过与半导体元件的电解质相连。 生物传感器可以被设计成使得仅昆虫天线的天线链成员，特别是单天线链部件的数量少，在直接化学感受器的一端或经由电解质与半导体器件连接。

公开(公告)号：WO1997003345A2

公开(公告)日：1997-01-30

申请号：PCT/GB1996001660

申请日：1996-07-12

Applicant: ROYAL FREE HOSPITAL SCHOOL OF MEDICINE ,  EMERY, Vincent, Clive ,  GRIFFITHS, Paul

Inventor： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE

IPC: G01N00/00

CPC classification number: C12Q1/705 ,  C12Q1/6851

Abstract: An isolated nucleic acid molecule comprising: (i) a primer portion consisting of a contiguous sequence of from 10 to 50 nucleotides capable of hybridising to (a) the target nucleic acid molecule represented by SEQ. ID No. 1, or (b) to the complementary stand thereof; and optionally (ii) a further portion comprising from 1 to 25 nucleotides joined to and immediately 5' to the 5' end of the primer portion.

Abstract translation: 一种分离的核酸分子，其包含：（i）引物部分，其由能够与（a）SEQ ID NO：1所代表的靶核酸分子杂交的10至50个核苷酸的连续序列组成。 ID号1，或（b）其互补立场; 和任选地（ii）包含1至25个核苷酸的另外部分，其连接到引物部分的5'末端并立即连接到5'末端。

公开(公告)号：WO1996032628A2

公开(公告)日：1996-10-17

申请号：PCT/US1996004726

申请日：1996-04-04

Applicant: AMRESCO, INC.

Inventor： AMRESCO, INC. ,  HIGHMAN, Timothy, J. ,  SMERDEL, Michael, E. ,  BEHM, Stephen, M.

IPC: G01N00/00

CPC classification number: G01N27/44747 ,  Y10T436/255

Abstract: Improved polyacrylamide-type gels utilizing diacrylyl tertiary amide crosslinkers are disclosed having relatively high monomer: crosslinker ratios in comparison to conventional polyacrylamide gels. The resulting gels have pore structures chemically and physically different from conventional polyacrylamide gels and, when prepared in the optimal monomer: crosslinker ratio, provide superior resolution and structural characteristics over the conventional polyacrylamide gels.

Abstract translation: 公开了使用二酰基酰胺交联剂的改进的聚丙烯酰胺型凝胶，与传统的聚丙烯酰胺凝胶相比具有相对较高的单体：交联剂比例。 所得凝胶具有与常规聚丙烯酰胺凝胶化学和物理不同的孔结构，并且当以最佳单体：交联剂比制备时，提供比常规聚丙烯酰胺凝胶更好的分辨率和结构特征。

公开(公告)号：WO1999004238A2

公开(公告)日：1999-01-28

申请号：PCT/US1998014566

申请日：1998-07-14

Applicant: MITOTIX, INC. ,  DEACONESS HOSPITAL ,  PAGANO, Michele ,  DRAETTA, Giulio ,  ROLFE, Mark ,  LODA, Massimo

Inventor： MITOTIX, INC. ,  DEACONESS HOSPITAL

IPC: G01N00/00

CPC classification number: G01N33/57496 ,  C12Q1/6886 ,  C12Q2600/118 ,  G01N2333/4739

Abstract: The present invention relates to diagnostic and prognostic assays which assess the phenotype and aggressiveness of a disorder by detecting the stability of a cyclin kinase inhibitor (CKI) protein.

Abstract translation: 本发明涉及通过检测细胞周期蛋白激酶抑制剂（CKI）蛋白的稳定性来评估疾病的表型和侵袭性的诊断和预后测定。

公开(公告)号：WO1998000695A2

公开(公告)日：1998-01-08

申请号：PCT/US1997011713

申请日：1997-07-03

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  SHARP, Phillip, A. ,  ZHOU, Qiang

Inventor： MASSACHUSETTS INSTITUTE OF TECHNOLOGY

IPC: G01N00/00

CPC classification number: C07K14/005 ,  A61K38/00 ,  C12N2740/16322

Abstract: The invention pertains to a novel transcriptional activity factor, Tat-Stimulatory Factor, as well as genes encoding this factor and fragments and biologically functional variants thereof. The Tat-Stimulatory Factor is involved in the regulation of transcriptional elongation of HIV-1 by Tat. The invention also pertains to therapeutics involving the foregoing proteins and genes, and agents that bind to the foregoing proteins and genes. The invention also relates to methods of screening for a compound which binds to Tat-SF1, Tat-SF1-associated kinase and/or a complex of Tat-SF1 and Tat-SF1-associated kinase, as well as methods of screening for compounds which modulate Tat-SF1-mediated transcriptional activation.

Abstract translation: 本发明涉及新的转录活性因子Tat-刺激因子，以及编码该因子的基因及其片段和生物功能变体。 Tat刺激因子涉及Tat调控HIV-1的转录延伸。 本发明还涉及涉及上述蛋白质和基因的治疗剂，以及结合前述蛋白质和基因的药剂。 本发明还涉及筛选结合Tat-SF1，Tat-SF1相关激酶和/或Tat-SF1和Tat-SF1相关激酶复合物的化合物的方法，以及筛选化合物的方法 调节Tat-SF1介导的转录激活。