公开(公告)号：WO1997017600A2

公开(公告)日：1997-05-15

申请号：PCT/US1996015356

申请日：1996-09-25

Applicant: INFECTECH, INC.

Inventor： INFECTECH, INC. ,  FELDER, Mitchell, S. ,  OLLAR, Robert-A.

IPC: G01N00/00

CPC classification number: C12Q1/18 ,  Y10S435/81 ,  Y10S435/975

Abstract: A method for determining the sensitivity of at least one nonparaffinophilic microorganism from a specimen obtained from a patient to different antimicrobial agents, and predetermined quantities thereof. The method includes providing at least one receptacle containing an aqueous solution and adjusting the solution to mimic the in vivo clinical conditions of said patient. The method further includes inoculating the solution with the specimen and placing into the receptacle (i) a slide coated with a carbon source and (ii) a predetermined quantity of an antimicrobial agent to be tested. By observing the nonparaffinophilic microorganism growth or lack thereof on the slide, it can be determined whether the predetermined quantity of the antimicrobial agent is effective in inhibiting growth of the nonparaffinophilic microorganism on the slide. An associated apparatus is also disclosed.

Abstract translation: 用于确定从患者获得的样品至不同抗微生物剂的至少一种非嗜碳性微生物的敏感性的方法及其预定量。 该方法包括提供至少一个含有水溶液的容器并调节溶液以模拟所述患者的体内临床状况。 该方法还包括用样品接种溶液并将其放入容器中（i）涂覆有碳源的载玻片和（ii）预定量的待测试的抗微生物剂。 通过观察载玻片上的非嗜嗜性微生物的生长或不存在，可以确定预定量的抗微生物剂是否有效抑制载玻片上的非嗜性微生物的生长。 还公开了一种相关联的装置。

公开(公告)号：WO1997038088A1

公开(公告)日：1997-10-16

申请号：PCT/US1997004017

申请日：1997-03-14

Applicant: INFECTECH, INC.

Inventor： INFECTECH, INC. ,  OLLAR, Robert-A.

IPC: C12N01/00

CPC classification number: C12N1/28 ,  C12M27/02 ,  C12M41/02 ,  C12M41/26 ,  C12P1/00 ,  C12R1/00

Abstract: A method of growing a paraffinophilic organism including providing a tank and introducing into the tank (i) sterile broth and (ii) a paraffin. The broth is then inoculated with a paraffinophilic organism that will grow and multiply in the broth by utilizing paraffin as its sole carbon source. An associated apparatus is also disclosed.

Abstract translation: 一种生长石蜡生物的方法，包括提供罐并引入罐（i）无菌肉汤和（ii）石蜡。 然后将肉汤接种在石蜡生物体上，通过利用石蜡作为其唯一的碳源，将在肉汤中生长和繁殖。 还公开了一种相关联的装置。

公开(公告)号：WO1997010357A1

公开(公告)日：1997-03-20

申请号：PCT/US1996013549

申请日：1996-08-23

Applicant: INFECTECH, INC.

Inventor： INFECTECH, INC. ,  OLLAR, Robert, A. ,  FELDER, Mitchell, S.

IPC: C12Q01/02

CPC classification number: C12Q1/24 ,  C12Q1/025 ,  C12Q1/18 ,  Y10S435/81 ,  Y10S435/975

Abstract: A method for determining the sensitivity of at least one nonparaffinophilic microorganism from a specimen obtained from a patient to different anticrobial agent and predetermined quantities thereof. The method includes providing at least one receptacle containing an aqueous solution and inoculating the solution with the specimen. The method further includes placing into the receptacle (i) a slide coated with a carbon source and (ii) a predetermined quantity of an antimicrobial agent to be tested. By observing the nonparaffinophilic microorganism growth or lack thereof on the slide, it can be determined whether the predetermined quantity of the antimicrobial agent is effective in inhibiting growth of the nonparaffinophilic microorganism on the slide. An associated apparatus is also disclosed.

Abstract translation: 用于确定从患者获得的样品中至少一种非嗜碳性微生物对不同抗微生物剂及其预定量的敏感性的方法。 该方法包括提供至少一个含有水溶液的容器并用该样品接种该溶液。 该方法还包括将容器（i）涂覆有碳源的载玻片和（ii）预定量的待测试的抗微生物剂。 通过观察载玻片上的非嗜性微生物生长或不存在，可以确定预定量的抗微生物剂是否有效抑制载玻片上非嗜性微生物的生长。 还公开了一种相关联的装置。

公开(公告)号：WO1991006669A1

公开(公告)日：1991-05-16

申请号：PCT/US1990005949

申请日：1990-10-16

Applicant: INFECTECH, INC.

Inventor： INFECTECH, INC. ,  OLLAR, Robert, A.

IPC: C12Q01/04

CPC classification number: C12Q1/18 ,  C12Q1/04 ,  G01N33/5695 ,  G01N2333/35 ,  Y10S435/863 ,  Y10S435/864 ,  Y10S435/975

Abstract: A method of speciating and identifying MAI in a specimen comprises placing a paraffin coated slide in a receptacle containing a sterile aqueous solution inoculated with the specimen, analyzing the slide after exposure to the specimen to determine the presence or absence of atypical Mycobacteria, and after the analysis step, if atypical Mycobacteria are determined to be present, performing at least one speciation assay to ascertain if the atypical Mycobacteria are MAI. A related apparatus is also disclosed for speciating and identifying MAI in a specimen comprising a paraffin-wax coated slide, a tube having a sterile aqueous solution contained therein, the tube adapted to hold the slide, and at least one speciation assay means for performing an assay to determine the presence or absence of MAI in the speciment after the speciment is introduced into the tube holding the solution and the slide. An apparatus and method for determining the sensitivity of MAI to different antibiotics and dosages thereof is also provided.

公开(公告)号：WO1997017424A1

公开(公告)日：1997-05-15

申请号：PCT/US1996015317

申请日：1996-09-25

Applicant: INFECTECH, INC.

Inventor： INFECTECH, INC. ,  FELDER, Mitchell, S. ,  OLLAR, Robert-A

IPC: C12M01/00

CPC classification number: C12Q1/18 ,  Y10S435/863

Abstract: A method of determining the sensitivity of at least one paraffinophilic microorganism from a specimen obtained from a patient to different antimicrobial agents and predetermined quantities thereof includes providing at least one receptacle containing an aqueous solution and adjusting the solution to mimic the in vivo clinical conditions of the patient. The method then includes inoculating the solution with the specimen and then placing into the receptacle (i) a paraffin coated slide to bait the at least one paraffinophilic microorganism and (ii) a predetermined quantity of an antimicrobial agent to be tested. The slide is then observed for paraffinophilic microorganism growth or lack thereof to determine whether the predetermined quantity of the antimicrobial agent is effective in inhibiting growth of the paraffinophilic microorganisms on the slide. An associated apparatus is also provided.

Abstract translation: 确定从患者获得的样品至不同抗微生物剂及其预定量的至少一种嗜酸性微生物的灵敏度的方法包括提供至少一个含有水溶液的容器并调节溶液以模拟体内临床条件 患者。 该方法然后包括用样品接种溶液，然后将其放入容器中（i）石蜡包裹的载玻片以诱饵至少一种嗜
温微生物和（ii）预定量的待测试的抗微生物剂。 然后观察载玻片以进行石蜡微生物生长或缺乏，以确定预定量的抗微生物剂是否有效抑制载玻片上的嗜石微生物的生长。 还提供了一种相关联的装置。

公开(公告)号：WO1997012957A1

公开(公告)日：1997-04-10

申请号：PCT/US1996015325

申请日：1996-09-25

Applicant: INFECTECH, INC.

Inventor： INFECTECH, INC. ,  OLLAR, Robert-A.

IPC: C12M01/00

CPC classification number: C12M23/08 ,  C12Q1/18 ,  G01N2333/35

Abstract: A method of determining the sensitivity of at least one paraffinophilic microorganism from a specimen obtained from a patient to different antimicrobial agents and predetermined quantities thereof includes providing at least one receptacle containing an aqueous solution and then inoculating the solution with the specimen. The method then includes placing into the receptacle (i) a paraffin coated slide to bait the at least one paraffinophilic microorganism and (ii) a predetermined quantity of an antimicrobial agent to be tested. The slide is then observed for paraffinophilic microorganism growth or lack thereof to determine whether the predetermined quantity of the antimicrobial agent is effective in inhibiting growth of the paraffinophilic microorganims on the slide. An associated apparatus is also provided.

Abstract translation: 确定从患者获得的样品至不同抗微生物剂及其预定量的至少一种嗜
温微生物的灵敏度的方法包括提供至少一个含有水溶液的容器，然后用该样品接种该溶液。 该方法然后包括将容器（i）置于石蜡包裹的载玻片上以诱饵至少一种嗜
温微生物和（ii）预定量的待测试的抗微生物剂。 然后观察载玻片以进行石蜡微生物生长或缺乏，以确定预定量的抗微生物剂是否有效抑制载玻片上的石蜡微生物的生长。 还提供了一种相关联的装置。

公开(公告)号：WO1997012056A1

公开(公告)日：1997-04-03

申请号：PCT/US1996014475

申请日：1996-09-10

Applicant: INFECTECH, INC.

Inventor： INFECTECH, INC. ,  OLLAR, Robert-A. ,  FELDER, Mitchell, S.

IPC: C12Q01/18

CPC classification number: C12Q1/18

Abstract: A method of automatically testing the sensitivity of a paraffinophilic microorganism to different antimicrobial agents and concentrations thereof includes a plurality of receptacles (2-22) each containing an amount of liquid medium, and antimicrobial agent to be tested and the microorganism to be tested. A paraffin containing slide (32-52) is placed in each receptacle (2-22) after which incubation is effected. A light scatter sensor (62) is employed to automatically and sequentially monitor the extent of microorganism growth on the slides (32-52) and the growth of each slide is employed to determine which antimicrobial agent and which concentration is effective to resist growth of the paraffinophilic microorganism. The slides (32-52) are moved sequentially in operative relationship with the light scatter sensor (62) while resisting rotational movement of the slides. Positive and negative control slides are preferably employed as is a computer (64) in receiving and processing the results of the slide testing. Related apparatus is disclosed.

Abstract translation: 自动测试嗜热微生物对不同抗微生物剂及其浓度的敏感性的方法包括多个容纳一定量的液体培养基的容器（2-22），待测试的抗微生物剂和待测试的微生物。 将含有石蜡的载玻片（32-52）放置在每个容器（2-22）中，然后进行孵育。 使用光散射传感器（62）自动并依次监测载玻片上微生物生长的程度（32-52），并且使用每个载玻片的生长来确定哪种抗微生物剂和哪种浓度有效地抵抗 嗜盐微生物。 滑动件（32-52）在抵抗滑动件的旋转运动的同时与光散射传感器（62）以可操作的关系依次移动。 优选使用正和负控制载玻片，与计算机（64）一样，用于接收和处理幻灯片测试的结果。 公开了相关装置。

公开(公告)号：WO1997017462A1

公开(公告)日：1997-05-15

申请号：PCT/US1996017878

申请日：1996-11-07

Applicant: INFECTECH, INC.

Inventor： INFECTECH, INC. ,  FELDER, Mitchell, S. ,  OLLAR, Robert-A.

IPC: C12Q01/02

CPC classification number: C12Q1/04 ,  Y10S435/81 ,  Y10S435/975

Abstract: A method of determining the presence or absence of a nonparaffinophilic microorganism in a specimen taken from a patient is disclosed. The method includes providing a receptacle containing an aqueous solution (13) and adjusting the solution to mimic the in vivo clinical conditions of the patient. The method further includes inoculating the solution with the specimen and then placing in the receptacle a slide (18) coated with a carbon source to bait the nonparaffinophilic microorganism. The slide (18) is then analyzed after exposure to the specimen to determine the presence or absence of the nonparaffinophilic microorganism. An associated apparatus is also disclosed. The front elevational view of a test tube (12) holding a slide coated with a carbon source in an aqueous solution (13) inoculated with a specimen.

Abstract translation: 公开了一种确定从患者取出的样本中存在或不存在非嗜性微生物的方法。 该方法包括提供含有水溶液（13）的容器并调节溶液以模拟患者的体内临床状况。 该方法还包括用样品接种溶液，然后将包含碳源的载玻片（18）放置在容器中以诱饵非嗜性微生物。 然后在暴露于样品之后分析载玻片（18）以确定是否存在或不存在非纯嗜性微生物。 还公开了一种相关联的装置。 在用试样接种的水溶液（13）中保持涂有碳源的载玻片的试管（12）的正视图。

公开(公告)号：WO1997010327A1

公开(公告)日：1997-03-20

申请号：PCT/US1996013564

申请日：1996-08-23

Applicant: INFECTECH, INC.

Inventor： INFECTECH, INC. ,  OLLAR, Robert, A. ,  FELDER, Mitchell, S.

IPC: C12M01/00

CPC classification number: C12Q1/14 ,  C12M23/08 ,  C12N1/26 ,  C12Q1/04 ,  Y10S435/81

Abstract: A method of determining the presence or absence of a nonparaffinophilic microorganism in a specimen taken from a patient includes providing a receptable containing an aqueous solution and inoculating the aqueous solution with the specimen. A slide coated with a carbon source is placed into the receptacle. By analyzing the slide after exposure to the specimen, the presence or absence of a nonparaffinophilic microorganism in the specimen can be determined. An associated apparatus is also disclosed.

Abstract translation: 确定从患者取出的样品中存在或不存在非嗜性微生物的方法包括提供含有水溶液的接受物并将样品接种在水溶液中。 将涂有碳源的载玻片放入容器中。 通过在暴露于样品后分析载玻片，可以确定样品中存在或不存在非嗜性微生物。 还公开了一种相关联的装置。

公开(公告)号：WO1994017200A1

公开(公告)日：1994-08-04

申请号：PCT/US1994000831

申请日：1994-01-21

Applicant: INFECTECH, INC. ,  OLLAR, Robert, A. ,  FELDER, Mitchell, S.

Inventor： INFECTECH, INC.

IPC: C12Q01/02

CPC classification number: C12Q1/04

Abstract: A method of determining the presence of a paraffinophilic organism in a body specimen involves introducing portions of the body specimen into a plurality of receptacles (50-57) which contain a sterile broth and antibiotics. Subsequently, one paraffin coated slide (18) is introduced into each receptacle. After observing organism growth on the paraffin coated slides, at least one slide is subjected to an alcohol-acid fastness test to determine whether the organism is an alcohol-acid fast, an acid-fast organism or a non-acid-fast/non-alcohol-acid fast organism. If it is determined that an alcohol-acid fast organism is present on the first slide, a tellurite reduction assay is performed on a second slide to determine the possibility of a presence of paraffinophilic organism on the second slide. If the tellurite reduction assay results in a determination that there is a possibility of presence of a paraffinophilic organism on the second slide, at least one speciation assay on the third paraffin coated slide is performed to confirm the presence of a paraffinophilic organism on the third paraffin coated slide. Subsequently, DNA extraction is employed on at least one additional slide to determine whether a paraffinophilic organism is present in the body specimen.

Abstract translation: 确定身体标本中存在石蜡生物体的方法包括将身体标本的部分引入到含有无菌液体和抗生素的多个容器（50-57）中。 随后，将一个石蜡涂布的载玻片（18）引入每个容器中。 观察石蜡涂层载玻片上的生物体生长后，至少一个载玻片进行酒精酸牢度试验，以确定生物体是否为快速，耐酸的生物体或非酸性快速/ 酒精酸快速生物。 如果确定第一载玻片上存在醇酸快速生物体，则在第二载玻片上进行碲酸盐还原测定以确定在第二载玻片上存在石蜡生物体的可能性。 如果臭氧层还原测定结果确定在第二载玻片上存在石蜡生物体的可能性，则进行在第三石蜡涂布的载玻片上的至少一种形态分析，以证实在第三石蜡上存在石蜡生物体 涂层幻灯片。 随后，在至少一个额外的载玻片上采用DNA提取，以确定身体样品中是否存在石蜡生物体。