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公开(公告)号:WO2021221752A2
公开(公告)日:2021-11-04
申请号:PCT/US2021/016870
申请日:2021-02-05
IPC分类号: C12Q1/28 , C12Q1/32 , C40B40/02 , G01N27/327 , C12N15/53 , C12N15/1086 , C12N15/52 , C12Q1/005 , G01N27/3272 , G01N27/3274
摘要: Described herein are systems, assays, methods and compositions for identification of oxidase microbial redox-enzymes (MREs) specific to an analyte of interest from an environmental source. The technology relates to identification of analyte-responsive MREs that can quantify the concentration of a target analyte with high specificity and high sensitivity, for example, where the identified analyte-responsive redox-enzyme can be used to engineer an electrochemical biosensor.
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公开(公告)号:WO2022040529A2
公开(公告)日:2022-02-24
申请号:PCT/US2021/046906
申请日:2021-08-20
发明人: BILLADEAU, Mark , CARBONE, Nicholas , CLINTON, Charles , DOWDELL, Scott , KOCHAR, Manish , FOX-LYON, Nicholas , JEFFREY-COKER, Bandele , TUCKER-SCHWARTZ, Alexander , SIGAL, George , SPIELES, Gisbert , VANDERSARL, Jules , WOHLSTADTER, Jacob
IPC分类号: G01N21/66 , G01N27/403 , G01N21/76 , G01N21/69 , B01L3/00 , C12Q1/00 , G01N33/543 , B01L2300/0645 , B01L2300/161 , B01L3/5085 , G01N27/3272 , G01N27/3273 , G01N27/3277 , G01N27/49
摘要: An electrochemical cell (100) includes a plurality of working electrode zones (104) disposed, and defining a pattern, on a surface of the cell and at least one auxiliary electrode (102) disposed on the surface. The auxiliary electrode may have a defined interfacial potential.
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公开(公告)号:WO2022029160A2
公开(公告)日:2022-02-10
申请号:PCT/EP2021/071738
申请日:2021-08-04
IPC分类号: B01L3/00 , G01N33/543 , H01M8/16 , B01L2300/0636 , B01L2300/0645 , B01L2300/0867 , B01L3/5027 , G01N27/3272 , G01N27/3278 , G01N33/5438
摘要: Microfluidic device (1) comprising at least one fluid channel (2) comprising at least one inlet (3), wherein said at least one fluid channel (2) is fluidly connected to a first sensor (4) downstream of said at least one fluid inlet (3), wherein the first sensor (4) comprises at least one sensor cathode (5) and at least one sensor anode (6) formed on an essentially electrically isolating substrate (7), wherein the sensor cathode (5) and the sensor anode (6) are spaced apart by a gap (8) formed on the electrically isolating substrate (7), and wherein at least one analyte capturing molecule (9) is immobilized in the gap (8) on the substrate (7), wherein the at least one capturing molecule (9) is adapted to capture at least one analyte (10) of a fluid sample introduced into the inlet (3) and transported to the first sensor (4).
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公开(公告)号:WO2021252124A2
公开(公告)日:2021-12-16
申请号:PCT/US2021/032180
申请日:2021-05-13
IPC分类号: A61B5/145 , A61B5/1486 , A61B5/1473 , G01N27/327 , A61B5/14532 , A61B5/14546 , A61B5/14735 , A61B5/14865 , G01N27/3272
摘要: Analyte sensors are being increasingly employed for monitoring various analytes in vivo. Analyte sensors configured to monitor multiple analytes are also in development. Sufficient sensitivity for low-abundance analytes and multiple analytes having differing membrane permeability values may complicate analyte detection in some cases. Analyte sensors may feature enhancements to address one or both of these issues. Some analyte sensors may comprise a carbon working electrode comprising a dielectric substrate, one or more apertures extending through the dielectric substrate and filled with a carbon conductor pillar, a carbon conductor coating on a first face of the dielectric substrate in direct contact with each carbon conductor pillar, and one or more active areas on a second face of the dielectric substrate in electrical communication with the carbon conductor pillars. Photopolymerized mass transport limiting membranes may be used in combination with such carbon working electrodes or with other working electrode types.
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公开(公告)号:WO2021133903A1
公开(公告)日:2021-07-01
申请号:PCT/US2020/066826
申请日:2020-12-23
发明人: LATOUR, John, V. , MCCANLESS, Jonathan, D. , OJA, Stephen , OUYANG, Tianmei , WALLIS, Kevin, Paul , FELDMAN, Benjamin, J. , HOSS, Udo , QIAN, Suyue
IPC分类号: G01N27/327 , A61B5/145 , G01N33/66 , A61B5/14532 , A61B5/14865 , C12Q1/004 , C12Q1/006 , G01N27/3272 , G01N27/3277 , G01N27/3278
摘要: Analyte sensors responsive at low working electrode potentials may comprise an active area upon a surface of a working electrode, wherein the active area comprises a polymer, a redox mediator covalently bonded to the polymer, and at least one analyte-responsive enzyme covalently bonded to the polymer. A specific redox mediator responsive at low potential may have a structure of (I) wherein G is a linking group covalently bonding the redox mediator to the polymer. A mass transport limiting membrane permeable to the analyte may overcoat the active area. In some sensor configurations, the mass transport limiting membrane may comprise a membrane polymer crosslinked with a branched crosslinker comprising three or more crosslinkable groups, such as polyethylene glycol tetraglycidyl ether.
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公开(公告)号:WO2021133654A1
公开(公告)日:2021-07-01
申请号:PCT/US2020/065874
申请日:2020-12-18
发明人: MCKINLAY, Robert , LIN, Chi-En , DANG, Tri T.
IPC分类号: G01N27/327 , A61B5/1486 , A61B2562/0285 , A61B2562/12 , A61B5/14532 , A61B5/14546 , A61B5/14865 , A61L2/206 , A61L2/232 , C12Q1/006 , C12Q1/32 , G01N27/3272
摘要: An analyte biosensor is provided having an analyte biosensing layer and an ethylene oxide absorption layer. The ethylene oxide absorption layer is provided over the analyte biosensing layer. A method is also provided.
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公开(公告)号:WO2021123178A1
公开(公告)日:2021-06-24
申请号:PCT/EP2020/087036
申请日:2020-12-18
发明人: DETLOFF, Andreas , HUMBEK, Egon , LETKEMANN, Artur
IPC分类号: B32B38/18 , B32B41/00 , B32B37/02 , B32B38/10 , B32B2309/72 , B32B2535/00 , B32B37/20 , B32B38/0004 , B32B38/185 , G01N27/3272
摘要: A method of producing a plurality of analytical test strips (146) by using a reel-to-reel process is disclosed. Each analytical test strip (146) comprises at least one first layer (198) having at least one first electrode (200), at least one spacer layer (202) and at least one second layer (204) having at least one second electrode (206). The method comprises: i. providing at least one continuous first layer web (114), having disposed on a first side (118) at least one first electrode layer (120), the first layer web (114) having a first layer edge (122); ii. continuously disposing, in at least one first lamination station (116), at least one continuous spacer layer web (124) onto the first side (118) of the first layer web (114), wherein the spacer layer web (124) has a spacer layer edge (126), wherein the disposing is position-controlled in a master-slave fashion by using a position of the first layer edge (122) as a master position and a position of the spacer layer edge (126) as a slave position, wherein the disposing takes place such that the spacer layer edge (126) is offset from the first layer edge (122) and that, thereby, a portion of the first side (118) of the first layer (114) and a portion of the first electrode layer (120) remain uncovered by the spacer layer web (124); and iii. continuously disposing, in at least one second lamination station (128), at least one continuous second layer web (130) onto the spacer layer web (124), the second layer web (130) having disposed on a first side (132) at least one second electrode layer (134), wherein the disposing is performed such that the second electrode layer (134) faces the first layer web (114), wherein the second layer web (130) has a second layer edge (136), wherein the disposing takes place such that the second layer edge (136) is aligned with the first layer edge (122), and wherein the disposing is position- controlled in a master-slave fashion by using a position of the second layer edge (136) as a slave position. Further disclosed are a cutting station (172) and a manufacturing system (110) for producing a plurality of analytical test strips (146) by using a reel-to-reel process.
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公开(公告)号:WO2016073395A8
公开(公告)日:2016-05-12
申请号:PCT/US2015/058705
申请日:2015-11-03
发明人: BEATY, Terry , BUCK, Harvey , DIEBOLD, Eric , GERBER, Martin
IPC分类号: H01M8/04 , C12Q1/006 , G01N27/3272 , G01N27/3273 , G01N27/3274 , G01N33/49 , G01N33/66
摘要: Electrode arrangements for test elements, test elements and methods of determining sample sufficiency, monitoring fill time, establishing fill directions and/or confirming electrode coverage by a sample for test elements are disclosed. The test elements have an electrode-support substrate including a spacer having an edge defining a boundary of a capillary channel. The electrode-support substrate also includes a first electrode pair and a second electrode pair, wherein the first electrode pair is positioned between the second electrode pair. The method includes dosing the test sensor with the fluid sample; applying a signal to the first electrode pair and the second electrode pair, detecting a first response to the signal from the first electrode pair, and detecting a second response to the signal from the second electrode pair; determining a time period between the first response and the second response
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