公开(公告)号：WO2022264645A1

公开(公告)日：2022-12-22

申请号：PCT/JP2022/016338

申请日：2022-03-30

Applicant: 株式会社島津製作所 ,  国立大学法人熊本大学 ,  株式会社アイスティサイエンス

Inventor： 國澤　研大 ,  服部　考成 ,  渡邉　淳 ,  富澤　一仁 ,  永芳　友 ,  佐々野　僚一 ,  浅井　智紀

IPC: C12N15/10 ,  C12Q1/58 ,  C12Q1/62 ,  C12Q1/6806 ,  C12Q1/6872 ,  G01N33/569 ,  B01J20/287 ,  G01N27/62 ,  G01N30/26 ,  G01N30/34 ,  G01N30/50 ,  G01N30/72 ,  G01N30/86 ,  G01N30/88

Abstract: 本発明の修飾ヌクレオシドの分析方法は、修飾によって疎水性が増大する修飾ヌクレオシドである目的成分と、前記目的成分と異なる成分である参照成分とを含む試料を液体クロマトグラフィ用のカラムに導入し、移動相を構成する複数の溶媒の混合比を時間経過とともに変化させるグラジエント溶離により、前記目的成分及び前記参照成分を互いに分離して前記カラムから溶出する溶出工程と、前記目的成分及び前記参照成分をそれぞれ質量分析法により検出する工程と、前記目的成分の検出値と前記参照成分の検出値の比を算出する工程とを有する。前記溶出工程では、前記目的成分が前記カラムから溶出する第１期間と、前記参照成分が前記カラムから溶出する第２期間との間に、前記カラムの出口において、前記移動相を構成する前記溶媒の混合比の変化率が、前記第１期間及び前記第２期間のそれぞれにおける前記移動相を構成する前記溶媒の混合比の変化率よりも大きい第３期間を有するように、前記カラムに導入する前記移動相を構成する前記溶媒の混合比を変化させる。

公开(公告)号：WO2022245566A1

公开(公告)日：2022-11-24

申请号：PCT/US2022/028299

申请日：2022-05-09

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： MACKINNON, Peter ,  JACKSON, Michael R.

IPC: G01N30/34

Abstract: Disclosed is a liquid chromatography system and mixer (100) for use therein that includes a first split 106 connected to an inlet (102), the first split branching the flow of fluid from the inlet into a first path (108) and a second path (110); a second split (112) connected to an outlet of the first path, the second split branching the first path into a third path (114) and a fourth path (116); and a third split (118) connected to an outlet of the second path, the third split branching the second path into a fifth path (120) and a sixth path (122). The first path and the second path are offset by a first predetermined volume, the third path and the fourth path are offset by a second predetermined volume, and the fifth path and the sixth path are also offset by the second predetermined volume.

公开(公告)号：WO2022169710A1

公开(公告)日：2022-08-11

申请号：PCT/US2022/014555

申请日：2022-01-31

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： RAYMOND, Timothy M. ,  MACKINNON, Peter ,  JACKSON, Michael R.

IPC: G01N30/30 ,  G01N30/34 ,  G01N30/88

Abstract: Described is a solvent manager for a liquid chromatography system. The solvent manager includes a temperature control module, a plurality of solvent reservoirs, a gradient proportioning valve and a degasser. The temperature control module maintains a substantially constant temperature of one or more of the plurality of solvent reservoirs, gradient proportioning valve and degasser. The temperature control module may include a thermal chamber having an enclosure with a thermally controlled internal environment and may also include an active thermal element such as one or more heaters. The enclosure may surround at least one of the plurality of solvent reservoirs, gradient proportioning valve and degasser. The temperature control module reduces the negative effects on solvent composition that result from temperature-dependent changes in solvent viscosities and solvent densities. Such changes affect the retention times of analytes eluted from the chromatographic column.

公开(公告)号：WO2021227233A1

公开(公告)日：2021-11-18

申请号：PCT/CN2020/101229

申请日：2020-07-10

Applicant: 山东齐都药业有限公司

Inventor： 王朋 ,  王娜 ,  廖信信 ,  宋彩香 ,  于黎鑫 ,  吕伟刚 ,  黄祝刚 ,  孙滨滨 ,  王艳敏 ,  王杉

IPC: G01N30/02 ,  G01N2030/047 ,  G01N30/06 ,  G01N30/34 ,  G01N30/8675

Abstract: 药品质量检测领域中一种盐酸莫西沙星原料及制剂中2个光降解杂质的检测方法。光降解杂质为光降解杂质1和光降解杂质2，检测方法包括以下步骤：(1)供试品溶液的制备；(2)对照品溶液的制备；(3)检测：取供试品溶液与对照品溶液各10μL分别注入高效液相色谱仪，进行梯度洗脱；其中，色谱条件如下：流动相：流动相A与流动相B的混合物，流动相A是0.1％磷酸水溶液，流动相B是甲醇；流速：0.95～1.05mL/min；柱温：43～47℃；色谱柱：C18柱；检测波长：250nm；盐酸莫西沙星原料及制剂中2个光降解杂质的检测方法具有专属性强、定量准确、灵敏度高、方法稳定的特点，对药品质量的检测限度提高到很高的标准。

公开(公告)号：WO2021191730A1

公开(公告)日：2021-09-30

申请号：PCT/IB2021/052165

申请日：2021-03-16

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： LEMBCKE, Jan ,  ARHEIDT, Dirk ,  KLEIN, Gernot

IPC: G01N30/34 ,  B01D15/14 ,  G01N35/10 ,  B01D15/10 ,  B01D15/12

Abstract: Disclosed is a solvent supply (200) provided for a chromatography system (10) comprising a mobile phase drive and a separation unit, wherein the mobile phase drive is configured for driving a mobile phase through the separation unit, and the separation unit is configured for chromatographically separating compounds of a sample fluid in the mobile phase. The solvent supply (200) comprises a tubing (250) having a tubing end (260) configured to couple to a solvent container (220) in order to supply solvent contained in the solvent container (220) via the tubing (250) to the chromatography system (10), wherein the tubing (250) is configured in a flexible manner in order to allow the tubing end (260) to be movable. A tubing arm (280) is supporting the tubing (250) in order to allow positioning the tubing end (260) into a first resting position (290, 290') wherein the tubing end (260) is at least substantially held in position and being distantly situated beyond a reference (230; 295) in order to prevent physical contact to the reference (230; 295).

公开(公告)号：WO2021186320A1

公开(公告)日：2021-09-23

申请号：PCT/IB2021/052125

申请日：2021-03-15

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： ROMANOWSKI, Thomas ,  MAAR, Frank ,  DOLBY, Bruce James ,  HUBER, Stefan

IPC: G01N30/86 ,  G01N30/88 ,  G01N35/00 ,  B01D15/14 ,  B01D15/16 ,  G01N30/20 ,  G01N30/24 ,  G01N30/34

Abstract: A sample separation network (100) which comprises a server node (102), a plurality of client nodes (104) communicatively coupled with the server node (102), a plurality of sample separation devices (106) communicatively coupled with the server node (102), wherein each of the sample separation devices (106) comprises device-specific control software (108) configured for controlling specifically the respectively assigned sample separation device (106), wherein at least one of the server node (102) and the client nodes (104) comprises generic control software (110) configured for generically controlling sample separation devices (106) in a non device-specific way, and wherein at least one of the server node (102) and the client nodes (104) and the sample separation devices (106') is configured for loading device-specific control software (108) from a sample separation device (106') to at least one of the server node (102) and the client nodes (104) upon connection of said sample separation device (106') to the sample separation network (100).

公开(公告)号：WO2021142137A1

公开(公告)日：2021-07-15

申请号：PCT/US2021/012523

申请日：2021-01-07

Applicant: REGENERON PHARMACEUTICALS, INC.

Inventor： MAO, Yuan ,  KLEINBERG, Andrew ,  ZHAO, Yunlong ,  GUO, Lili

IPC: G01N33/68 ,  G01N30/02 ,  B01D15/166 ,  B01D15/305 ,  G01N30/34 ,  G01N30/7233 ,  G01N33/6848 ,  G01N33/6857

Abstract: A method of enhancing a mass spectral signal is disclosed. The method can include contacting a sample to a separation column under conditions that permit sample components to bind to the substrate; applying a first mobile gradient to the separation column, wherein the first mobile phase gradient comprises trifluoroacetic acid (TFA) and a small molecule additive (e.g., an amino acid) or formic acid (FA) and a small molecule additive (e.g., an amino acid); applying a second mobile gradient to the separation column, wherein the second mobile phase gradient comprises TFA in acetonitrile (ACN) and a small molecule additive (e.g., an amino acid) or formic acid (FA) in ACN and a small molecule additive (e.g., an amino acid); and performing mass spectrometric analysis on eluted sample components.

公开(公告)号：WO2021034874A1

公开(公告)日：2021-02-25

申请号：PCT/US2020/046884

申请日：2020-08-19

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： HILL, Jason F. ,  GILAR, Martin ,  TARAFDER, Abhijit ,  DESJARDINS, Christopher

IPC: G01N30/34 ,  G01N30/86

Abstract: Described is a method for determining a dwell volume of a liquid chromatography system and a liquid chromatography system that can determined the system dwell volume. The method includes mixing a flow of a first solvent with a flow of a second solvent to form a solvent mixture. The flows of the first and second solvents are decreased and increased, respectively, to generate a gradient composition. A system pressure of the liquid chromatography system is measured to determine a pressure trace defined as the measured system pressure as a function of time. The dwell volume of the system is determined from a time delay determined between the gradient composition at the mixing location and the pressure trace. The method can be performed with a liquid chromatography system having a chromatographic column or a flow restrictor used in place of the chromatographic column.

公开(公告)号：WO2021028663A1

公开(公告)日：2021-02-18

申请号：PCT/GB2020/051888

申请日：2020-08-06

Applicant: FUJIFILM DIOSYNTH BIOTECHNOLOGIES UK LIMITED

Inventor： NAGY, Tibor ,  HAIGH, Jonathan ,  HEISE, Charles ,  TOPPING, Andrew ,  PULLEN, James

IPC: G01N30/34 ,  G01N30/46 ,  C07K1/36 ,  C12N15/10 ,  B01D15/18 ,  C12M1/00 ,  B01D15/16

Abstract: Apparatus for purifying a liquid comprising a target substance comprising at least two units arranged in series such that the feed stream of the second and any subsequent units comprises the product stream from a downstream unit, wherein each unit comprises specified components (i) to (vi), including a a switchable bypass assembly. Also claimed are the units and a flowpath assembly. The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

公开(公告)号：WO2019167011A1

公开(公告)日：2019-09-06

申请号：PCT/IB2019/051661

申请日：2019-03-01

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： PLACHETKA, Clemens

IPC: F04B13/02 ,  F04B23/04 ,  B01D15/16 ,  G01N35/10 ,  G01N30/32 ,  G01N30/34

Abstract: Flüssigchromatografie-Fluidversorgungsvorrichtung (150) zum Bereitstellen einer Mischung aus mehreren unterschiedlichen Fluiden als mobile Phase für ein Flüssigchromatografie-Probentrenngerät (10), wobei die Fluidversorgungsvorrichtung (150) eine Mehrzahl von Zuführleitungen (104 bis 107), von denen jede fluidisch mit einer jeweiligen von einer Mehrzahl von Fluidkomponentenquellen (100 bis 103) zum Bereitstellen eines jeweiligen der Fluide fluidisch gekoppelt ist, eine Mehrzahl von Fluidpumpen (51 bis 54), von denen jede einer jeweiligen der Zuführleitungen (104 bis 107) zugeordnet ist, und einen Vereinigungspunkt (87) aufweist, an dem die Fluide aus den Zuführleitungen (104 bis 107) zu vereinigen sind, wobei jede der Mehrzahl von Fluidpumpen (51 bis 54) abhängig von einem jeweiligen Schaltzustand der Fluidversorgungsvorrichtung (150) ein jeweiliges Fluid aus einer jeweiligen der Fluidkomponentenquellen (100 bis 103) zu dem Vereinigungspunkt (87) fördert oder nicht.