公开(公告)号：WO2023092139A2

公开(公告)日：2023-05-25

申请号：PCT/US2022/080304

申请日：2022-11-22

Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

Inventor： SIMS, Peter, Alan ,  BAYANDORIAN, Hovig

IPC: C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N27/26 ,  G01N33/487 ,  B82Y15/00

Abstract: The present disclosure provides systems and methods for isolation of nucleic acids. In particular, provided herein are nanopore-based or substrate-based sequencing systems and methods of use thereof for isolation of desired nucleic acids from a mixed library containing both accurate and inaccurate strands.

公开(公告)号：WO2023079202A1

公开(公告)日：2023-05-11

申请号：PCT/ES2022/070703

申请日：2022-10-31

Applicant: UNIVERSITAT POLITÈCNICA DE VALÈNCIA ,  UNIVERSITAT DE VALÈNCIA ,  FUNDACIÓN PARA EL FOMENTO DE LA INVESTIGACIÓN SANITARIA Y BIOMÉDICA DE LA COMUNITAT VALENCIANA

Inventor： MARTÍNEZ BISBAL, María Carmen ,  PINAZO DURÁN, María Dolores ,  MARTÍNEZ MAÑEZ, Ramón ,  BOTELLO MARABOTTO, Marina Dolores ,  SANZ GONZÁLEZ, Silvia María

IPC: G01N33/487

Abstract: La invención se refiere a un método in vitro para la detección precoz, diagnóstico, pronóstico y/o monitorización de la GPAA en un sujeto, caracterizado porque comprende las siguientes etapas: realización de un análisis metabolómico mediante espectroscopía de RMN de alto campo de una muestra biológica obtenida a partir de un sujeto, y procesado del espectro obtenido para obtener un resultado que indique si el sujeto será positivo o negativo para GPAA.

公开(公告)号：WO2023049097A1

公开(公告)日：2023-03-30

申请号：PCT/US2022/044089

申请日：2022-09-20

Applicant: THE PENN STATE RESEARCH FOUNDATION

Inventor： GUAN, Weihua ,  DONG, Ming ,  TANG, Zifan

IPC: C12Q1/34 ,  G01N33/487

Abstract: A method of counting target salivary miRNAs related to mild traumatic brain injury (mTBI) includes the steps of binding the miRNAs to padlock probes specific to each miRNA forming a hybridized complex for each miRNA, ligating the hybridized complex forming a closed circular structure, elongating the hybridized complex producing an elongated ssDNA amplicon via rolling circle amplification (RCA) elongation, measuring the concentration of elongated ssDNA amplicon according to a translocation event rate using a solid-state pore structure with a diameter greater than 10 nm, and determining initial concentrations of miRNAs based on the quantity of the initial miRNA molecule which is linear with the concentration of elongated ssDNA amplicon.

公开(公告)号：WO2023028329A1

公开(公告)日：2023-03-02

申请号：PCT/US2022/041712

申请日：2022-08-26

Applicant: AKADEUM LIFE SCIENCES, INC.

Inventor： WEGNER, Casey ,  LLOYD, William ,  MCNAUGHTON, Brandon H. ,  MACINTYRE, Brittany

IPC: B01D21/26 ,  G01N33/483 ,  G01N33/487 ,  G01N33/49

Abstract: A system for buoyant separation includes a separation container. Additionally or alternatively, the system can include an automated instrument, one or more processing components, and/or any other components. A method for buoyant separation can include any or all of: manipulating the separation container; adding materials to the separation container; removing materials form the separation container; otherwise processing the separation container; and/or any other processes.

公开(公告)号：WO2023026213A1

公开(公告)日：2023-03-02

申请号：PCT/IB2022/057936

申请日：2022-08-24

Applicant: FUNDACIÓN PARA LA INVESTIGACIÓN E INNOVACIÓN BIOSANITARIA DEL PRINCIPADO DE ASTURIAS (FINBA) ,  UNIVERSIDAD DE OVIEDO

Inventor： GUTIÉRREZ GUTIÉRREZ, Laura ,  ACEBES HUERTA, Andrea

IPC: A61K35/19 ,  A61M1/02 ,  A61P17/02 ,  B01D21/26 ,  C12N5/078 ,  G01N33/487

Abstract: The present invention relates to a unique method for obtaining secretomes from platelets and the secretome uses in regenerative medicine, in the treatment of diseases, including cell therapy, and in vitro cell culture. This method is developed with the aim of obtaining platelet-derived bio-products enriched in platelet granular cargo, rich in growth factors, and depleted of other platelet-derived molecules and plasma for universal use. The method avoids the use of platelet inhibitors or temperature-shock during the process, assuring the integrity of the platelet biological source. Furthermore, the potential processing of the product would allow additional clinical applications and solve storage limitations. This method and the characterization thereof of secretomes at the molecular level in health and disease will contribute to the identification of biomarkers for disease diagnosis and prognosis.

公开(公告)号：WO2023019471A1

公开(公告)日：2023-02-23

申请号：PCT/CN2021/113272

申请日：2021-08-18

Applicant: 成都齐碳科技有限公司

Inventor： 刘少伟 ,  何京雄 ,  李倩雯 ,  岳飞飞

IPC: C07K14/28 ,  C12N15/31 ,  C12Q1/6869 ,  G01N33/487

Abstract: 本发明属于靶分析物特性的表征技术领域，具体提供了一种孔蛋白单体的突变体、包含其的蛋白孔、以及其检测靶分析物的应用，其中所述孔蛋白单体的突变体的氨基酸包括在对应SEQ ID NO:1的A74、P75、G76、N77、A78、T79、N80和F81的一个或多个位置处的突变。

公开(公告)号：WO2023018398A1

公开(公告)日：2023-02-16

申请号：PCT/TR2022/050833

申请日：2022-08-10

Applicant: İZMİR YÜKSEK TEKNOLOJİ ENSTİTÜSÜ

Inventor： TEKİN, Hüseyin Cumhur ,  DELİKOYUN, Kerem

IPC: C12N13/00 ,  C12Q1/00 ,  G01N15/06 ,  G01N15/10 ,  G01N27/00 ,  G01N33/487

Abstract: The invention is related to an artificial neural network allowing cell groups to separate if their masses are close to each other or the same in addition to detecting target cells and used by training on the basis of learning based on cell morphologies to actively separate, and accordingly active separation of target cells under flow in the conventional magnetic levitation system used for the separation of cells in biological fluid samples based on their density differences.

公开(公告)号：WO2023278781A1

公开(公告)日：2023-01-05

申请号：PCT/US2022/035837

申请日：2022-06-30

Applicant: ILLUMINA, INC.

Inventor： MUSA, Rean Silke ,  FLANNERY, Anthony ,  BOYANOV, Boyan ,  COBURN, Nigel ,  MINASSIAN, Sharis

IPC: G01N27/327 ,  G01N27/414 ,  G01N33/487 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N33/48721

Abstract: Devices for sequencing biopolymers, methods of manufacturing the devices, and methods of using the devices are disclosed. In one example, such a device has a nanopore and a horizontal nanochannel. In some embodiments, the horizontal nanochannel may take a tortuous path. In some embodiments, such a device includes gas or air bubble generators or pressure pulse generators to block or unblock the horizontal nanochannel.

公开(公告)号：WO2022271795A1

公开(公告)日：2022-12-29

申请号：PCT/US2022/034479

申请日：2022-06-22

Applicant: CLICKBIO, INC.

Inventor： HARDINGHAM, Jonathan, D. ,  CHAPMAN, Quinn, Curtis James Wilson ,  GAGLIANO, Jessica, S.

IPC: B01L3/00 ,  B65D81/05 ,  G01N33/487 ,  B65D1/02 ,  B65D5/72

Abstract: Described herein are various liquid reservoirs that may be used in a laboratory setting. The liquid reservoirs minimize waste of pipetted liquid sample or reagents and may be used, e.g., in combination with a multi- well plate such as in NGS processes. Other reservoirs include those configured for compatibility with centrifuge rotors or multichannel pipettes with 12 or more channels.

公开(公告)号：WO2022263902A1

公开(公告)日：2022-12-22

申请号：PCT/IB2021/055426

申请日：2021-06-21

Applicant: GOLMOHAMMADI GHANE, Hamed ,  ARDALAN, Sina

Inventor： GOLMOHAMMADI GHANE, Hamed ,  ARDALAN, Sina

IPC: A61B5/00 ,  G01N21/64 ,  G01N33/487

Abstract: A system may be introduced for detecting biomarkers in a sweat sample. The system may include a skin patch and a fluorescence detector. The skin patch may include an adhesive tape with a central hole. The skin patch may further include a fluorescence sensor removably attached on the adhesive tape. The skin patch may further include at least one cotton thread. The fluorescence detector may include a light-tight chamber. The fluorescence detector may further include a sample holder removably disposed within the light-tight chamber. The fluorescence detector may further include at least one UV-LED light source may be positioned above the sample holder. The fluorescence detector may further include at least a light filter may be disposed within the light-tight chamber above the UV-LED light source. The fluorescence detector may further include an image capturing device may be disposed within the light-tight chamber.