The invention discloses a prediction method for slab deformation resistance in rolling process, and belongs to the technical field of steel rolling. The prediction method includes that deformation resistance is disintegrated into four independent parameters of reference yield stress, deformation temperature influence coefficient, deformation rate influence coefficient and deformation degree influence coefficient, and the four independent parameters are respectively determined; thermal simulation experiment is adopted in the reference yield stress to measure the deformation stress under the condition of deformation temperature being 1000 DEG C, deformation degree being 10%, deformation speed being 10s-1; and relationship between the deformation stress and respective changes of the deformation temperature influence coefficient, the deformation rate influence coefficient and the deformation degree influence coefficient in a certain scope is determined to obtain a general formula describing the deformation resistance. By the prediction method, a full set of test methods determining material deformation resistance is established, and is fully applicable to changes of rolling temperature, deformation degree and deformation rate in a large scope; and besides, determination test is simple and feasible, and precision of results is high.