The invention discloses a bus operation method and a bus operation system supporting line section dynamic regulation. The bus operation method comprises the steps of obtaining GPS (Global Positioning System) positioning data of a bus by a vehicle-mounted terminal at regular times during a section operation process of the bus, and comparing the GPS positioning data with a pre-stored longitude and latitude range of each station; carrying out station broadcasting on a station by the vehicle-mounted terminal after the vehicle-mounted terminal judges that the bus drives into the longitude and latitude range of one station according to a comparing result; determining an imminent station after the station according to sequence information, which is previously sent by a scheduling terminal and is recorded in a section operation station sequence list, of each sequentially-passed station during one roundtrip driving process of the bus in a section, and carrying out pre-arrival station broadcasting of the imminent station. According to the bus operation method disclosed by the invention, the line section dynamic regulation can be supported, and the travel demands of passengers can be better met when the bus is in section operation.