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CN104789594A 稳定高效表达人血清白蛋白和白介素Ⅱ融合蛋白的CHO细胞株的构建方法 无效 - 驳回

稳定高效表达人血清白蛋白和白介素Ⅱ融合蛋白的CHO细胞株的构建方法
摘要：
一种稳定高效表达人血清白蛋白和白介素Ⅱ融合蛋白的CHO细胞株的构建方法，通过将带有人血清白蛋白和人白介素Ⅱ融合基因的质粒电转至CHO细胞中，获得稳定、高效表达人源重组蛋白的CHO单克隆细胞株。本发明获得的单克隆细胞株可以分泌表达人血清白蛋白和人白介素Ⅱ融合蛋白，蛋白表达量较高，经分离纯化所获得融合蛋白，两种融合蛋白体外生物活性均比等摩尔单体IL-2活性要高，将成为广泛应用于肿瘤、肝炎、肺炎和免疫缺陷性疾病等多种疾病治疗的药物。
摘要（英）：

The invention discloses a method for constructing a CHO cell strain for stably and efficiently expressing a human serum albumin and an interleukin II fusion protein. The CHO monoclonal cell strain for stably and efficiently expressing the human-derived recombinant protein can be obtained by electrically transferring a plasmid with the human serum albumin and the interleukin II fusion gene into the CHO cell. The monoclonal cell strain obtained by the invention is capable of secretory expression of the human serum albumin and the interleukin II fusion protein, the protein expression quantity is high, and the fusion protein can be obtained through the separation and purification; the in vitro biological activities of two fusion proteins are higher than the mol monomer 1L-2 activity. The CHO cell strain can be widely applied to the medicines for treating various diseases such as tumor, hepatitis, pneumonia and immunodeficiency disease.

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