The invention discloses a fungus gibberella intermedia CA3-1 producing keratinase and an application method thereof. The activity of fungal keratinase after preliminary fermentation optimization can be 109U/mL. The enzyme has high activity toward keratin and casein and low activity toward collagen. SDS has an enzyme activity promoting function. Produced keratinase can independently complete or help detergents complete removing stains on cotton cloths, thus improving the washing efficiency. Meanwhile, fungal keratinase can complete sheepskin unhairing treatment. Obtained gibberella intermedia producing fungal keratinase has a good application prospect in the fields of washing, tanning and textiles.