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发明公开
CN105641693A 一种牛病毒性腹泻/黏膜病-牛传染性鼻气管炎二联灭活疫苗的生产方法 无效 - 撤回

一种牛病毒性腹泻/黏膜病-牛传染性鼻气管炎二联灭活疫苗的生产方法
摘要：
本发明涉及一种牛病毒性腹泻-牛传染性鼻气管炎二联灭活疫苗及其生产方法。本发明所述牛病毒性腹泻-牛传染性鼻气管炎二联灭活疫苗是采用适应微载体悬浮培养细胞MDBK的方法繁殖BVDV NMG株病毒和IBRV LY株病毒，该方法能够很好的在微载体培养的细胞上培养病毒，该方法解决微载体培养细胞繁殖病毒过程出现的载体丢失、接毒不均匀甚至没毒价获得较高效价的病毒液，其TCID50大于7.5，并且微载体损失量非常小，细胞生长均匀，是一个值得推广应用的方法。
摘要（英）：

The invention relates to a combined bovine viral diarrhoea(BVD)-infectious bovine rhinotracheitis(IBR) inactivated vaccine and a preparation method thereof. According to the combined BVD-IBR inactivated vaccine provided by the invention, a method adaptive to microcarrier suspension culture cells MDBK(Madin-Darby Bovine Kidney) is adopted for reproducing BVDV NMG virus and IBRV LY virus. The method can well culture virus on cells cultured by the microcarrier, and solves the problems that the virus solution has carrier loss, non-uniform virus inoculation and even a nontoxic value with higher valence in the process of reproducing virus by the microcarrier cultured cells, the TCID50 is greater than 7.5, the microcarrier loss amount is small, cell growth is uniform, so the method is worth popularization and application.

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