The present invention discloses a transmission shifting mechanism fatigue endurance test method. The method comprises the following steps: the transmission shifting mechanism is fixed according to the actual vehicle installation location mode; the load being the same as the actual vehicle is applied on the shifting finger of the shifting mechanism to drive the shifting arm of the shifting mechanism to complete the shifting operation and detect the turning angle of the shifting finger of the shifting mechanism at present, if the turning angle is in the range of the qualified turning angle when the shifting of the shifting mechanism is performed, the shifting is successful to accumulate one time to the total shifting number of the times and return the shifting finger of the shifting mechanism back to the neutral gear position, and the operation steps mentioned above are repeated until the total shifting number of times reaches a regular test number of times or beyond the range of the e qualified turning angle when the shifting of the shifting mechanism is performed. The operation is simple, the test result is objective and reliable, the simulation precision is high, and the universality is high.