The invention provides a method for separating and purifying a high-F value oligopeptide crude liquor. The method comprises the following steps of (1) preprocessing; (2) performing enzymolysis; (3) performing ultrafiltration; (4) performing activated carbon dearomatization; (5) performing gel chromatography; and (6) performing HPLC (High Performance Liquid Chromatography) purification. The method has the beneficial effects that activated carbon is relatively low in cost, does not cause pollution in a production process and is easy to recycle. As the surface of the granular active carbon is irregular in shape and is relatively complex in the pore structure, the active carbon is relatively good in adsorption effect to free aromatic amino acids and is relatively high in dearomatization effect, and meanwhile, a very good deodorization effect can be achieved, and the quality of high-F value oligopeptide is effectively improved; as nanometer aluminum oxide and nanocrystalline iron oxide are doped in the active carbon, the adsorption effect to free aromatic amino acids can be increased, and the F value of the oligopeptide is improved; and the separating and purifying method provided by the invention is low in cost, simple in operation, green and safe, and is suitable for mass production.